A5103168886- RNA Interference and Gene Delivery
- Regulation of Appetite and Obesity
- Advanced Drug Delivery Systems
- Biochemical Analysis and Sensing Techniques
- Nerve injury and regeneration
- Adipokines, Inflammation, and Metabolic Diseases
- Nanoparticle-Based Drug Delivery
- Lipid Membrane Structure and Behavior
- Parkinson's Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Zebrafish Biomedical Research Applications
- Graphene and Nanomaterials Applications
- Graphene research and applications
- Vagus Nerve Stimulation Research
- Carbon Nanotubes in Composites
- Neuropeptides and Animal Physiology
- Neurogenesis and neuroplasticity mechanisms
- Advanced Polymer Synthesis and Characterization
- Conducting polymers and applications
- Nanoplatforms for cancer theranostics
- Toxin Mechanisms and Immunotoxins
- Nanoparticles: synthesis and applications
- Fullerene Chemistry and Applications
- Biological Research and Disease Studies
- Phytochemistry and biological activities of Ficus species
University of North Carolina at Chapel Hill
2012-2021
Tohoku University
2020-2021
Lomonosov Moscow State University
2008-2014
University of Washington
2014
VA Puget Sound Health Care System
2014
University of Nebraska Medical Center
2008-2011
TU Dresden
2010
Geriatric Research Education and Clinical Center
2008-2010
Saint Louis University
2010
St. Louis VA Medical Center
2010
Schisantherin A (SA) is a promising anti-Parkinsonism natural product. However, its poor water solubility and rapid serum clearance impose significant barriers to delivery of SA the brain. This work aimed develop in nanoparticle formulation that extended circulation bloodstream consequently an increased brain uptake thus be potentially efficacious for treatment Parkinson's disease (PD). Spherical nanoparticles with mean particle size 70 nm were prepared by encapsulating into methoxy...
Superoxide dismutase 1 (SOD1) efficiently catalyzes dismutation of superoxide, but its poor delivery to the target sites in body, such as brain, hinders use a therapeutic agent for superoxide-associated disorders. Here enhance SOD1 across blood-brain barrier (BBB) and neurons enzyme was conjugated with poly(2-oxazoline) (POx) block copolymers, P(MeOx-b-BuOx) or P(EtOx-b-BuOx), composed (1) hydrophilic 2-methyl-2-oxazoline (MeOx) 2-ethyl-2-oxazoline (EtOx) (2) hydrophobic 2-butyl-2-oxazoline...
A variety of compounds will distribute into the brain when placed at cribriform plate by intranasal (i.n.) administration. In this study, we investigated ability albumin, a protein that can act as drug carrier but is excluded from blood-brain barrier, to after i.n. We labeled bovine serum albumin with [(125)I] ([(125)I]Alb) and studied its uptake 11 regions entry blood 5 minutes 6 hours [(125)I]Alb was present throughout minutes. Several showed distinct peaks in ranged (parietal cortex) 60...
Brain-derived neurotrophic factor (BDNF) is identified as a potent neuroprotective and neuroregenerative agent for many neurological diseases. Regrettably, its delivery to the brain hampered by poor serum stability rapid clearance. Here, novel nanoformulation reported composed of bio-compatible polymer, poly(ethylene glycol)-b-poly(L-glutamic acid) (PEG-PLE), that hosts BDNF molecule in nanoscale complex, termed here Nano-BDNF. Upon simple mixture, Nano-BDNF spontaneously forms uniform...
Several homopolymers, random copolymers and block based on poly(2-oxazoline)s (POx) were synthesized conjugated to horseradish peroxidase (HRP) using biodegradable nonbiodegradable linkers. These conjugates characterized by amino group titration, polyacrylamide gel electrophoresis (PAGE), isoelectric focusing, enzymatic activity assay conformation analysis. The contained average from about one two polymer chains per enzyme. From 70% 90% of was retained in most cases. Circular dichroism (CD)...
Leptin is a peptide hormone produced primarily by adipose tissue that acts as major regulator of food intake and energy homeostasis. Impaired transport leptin across the blood-brain barrier (BBB) contributes to resistance, which cause obesity. candidate for treatment this obesity limited because short half-life in circulation decreased BBB arises Chemical modification polypeptides with amphiphilic poly(ethylene oxide)-poly(propylene oxide) block copolymers (Pluronic) promising technology...
Schisantherin A (SA) is a promising anti-Parkinsonism Chinese herbal medicine but with poor water solubility and challenges to be delivered the brain. We formulated SA as nanocrystals (SA-NC), aiming improve its pharmacokinetic profile thus provide potential therapeutic agent for treatment of Parkinson's disease (PD). The rod-shaped SA-NC had particle size ∼160 nm 33.3% drug loading, exhibited fast dissolution rate in vitro. intact could internalized into Madin-Darby canine kidney (MDCK)...
Overexpression of the drug efflux transporter multidrug resistance-associated protein 2 (MRP2) in gastrointestinal tract and blood-brain barrier compromises oral delivery drugs to circulation system brain treatment Parkinson's disease (PD). In this study, we aim develop small-sized Pluronic P85/F68 micelles loaded with baicalein (B-MCs) overcome MRP2-mediated investigate related mechanism, as well anti-Parkinsonian efficacy. Spherical sustained-release B-MCs have a mean particle size 40.61...
Modification of a model protein, horseradish peroxidase (HRP), with amphiphilic block copolymer poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide) (Pluronic), was previously shown to enhance the transport this protein across blood−brain barrier in vivo and brain microvessel endothelial cells vitro. This work develops procedures for synthesis characterization HRP Pluronic copolymers, having different lengths (PEO) poly(propylene (PPO) blocks. Four monoamine derivatives (L81,...
In the present study, zebrafish was explored as an in vivo model to assess biofate of transferrin receptor (TfR)-targeted coumarin 6 (C6) micelles across various biological barriers. Three 7peptide (7pep)-decorated poly(ethylene glycol)-block-poly(ε-caprolactone) loaded with fluorescence (7pep–M–C6) different ligand densities were constructed particle sizes between 30 and 40 nm. Whole-mount immunostaining revealed that expression level TfR retina, brain, intestine increased along development...
Carbon nanotubes (CNTs) are novel materials with extraordinary mechanical properties. To gain insight on the design of high-mechanical-performance CNT-reinforced composites, optimal structure CNTs high nominal tensile strength was determined in this study, where values correspond to cross-sectional area entire specimen, including hollow core. By using machine learning-assisted high-throughput molecular dynamics (HTMD) simulation, relationship among following structural parameters/properties...
The relationship of geometrical properties and mechanical carbon nanotubes (CNTs) was investigated by using high-throughput molecular simulation. Geometrical such as diameter, number walls, chirality, crosslink density were considered. As a key factor in determining the composites reinforced with CNTs, nominal tensile strength is focus this study, which can be calculated fracture force divided full cross-sectional area including hollow core wall thickness. mode, strength, Young’s modulus...