A5029011087- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Long-Term Effects of COVID-19
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- COVID-19 Impact on Reproduction
- Neonatal and fetal brain pathology
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
- interferon and immune responses
- Infrared Thermography in Medicine
- Antimicrobial Peptides and Activities
- Epilepsy research and treatment
- Cytomegalovirus and herpesvirus research
- Phagocytosis and Immune Regulation
- Immune responses and vaccinations
- Cell Adhesion Molecules Research
- Cytokine Signaling Pathways and Interactions
- Mosquito-borne diseases and control
- Vagus Nerve Stimulation Research
- Reproductive System and Pregnancy
Duquesne University
2020-2024
UCLA Health
2024
Many neurotropic viruses cause more significant pathology in younger hosts as their brains are still developing. This experiment asked how central nervous system (CNS) viral-infections affect the development of synapses pediatric brain during infection and post-infection. Synaptogenesis is at its peak mice (10 days old) we hypothesized that a could disrupt synaptic proteins. We used transgenic mouse model where measles virus (MV) infects only mature neurons, leading us to question whether...
Abstract Extracellular vehicles ( EVs ) are an emerging class of drug carriers and primarily reported to be internalized into recipient cells via a combination endocytic routes such as clathrin-mediated, caveolae-mediated macropinocytosis pathways. In this work, 1 we investigated potential effects homotypic vs . heterotypic interactions by studying the cellular uptake homologous (EV donor same type) heterologous different types) 2 determined route EV internalization low...
Abstract Viral infections of the central nervous system (CNS) often cause disease in an age‐dependent manner, with greater neuropathology during fetal and neonatal periods. Transgenic CD46+ mice model these outcomes through a measles virus infection CNS neurons. Adult control viral spread survive interferon gamma (IFNγ)‐dependent whereas succumb despite similar IFNγ expression brain. Thus, we hypothesized that signaling adult brain may be more robust, potentially due to basal proteins. To...
Viral infections of the central nervous system (CNS) often cause worse neurological outcomes in younger hosts. Throughout childhood, brain undergoes extensive development and refinement to produce functional neural networks. Network function is maintained partly with help stem cells (NSCs) that replace neuronal glia subtypes two neurogenic niches (the hippocampus subventricular zone). Accumulating evidence suggests viruses disrupt NSC adulthood infancy, but vivo impact childhood on acute...
Abstract Lipidoid nanoparticles (LNPs) are the delivery platform in Onpattro, first FDA-approved siRNA drug. LNPs also carriers Pfizer-BioNTech and Moderna COVID-19 mRNA vaccines. While these applications have demonstrated that effectively deliver nucleic acids to hepatic muscle cells, it is unclear if could be used for of neural which notoriously challenging targets. Therefore, purpose this study was determine efficiently neurons. Because their potential utility either central nervous...
Abstract We have demonstrated, for the first time that microvesicles, a sub-type of extracellular vesicles (EVs) derived from hCMEC/D3: human brain endothelial cell (BEC) line transfer polarized mitochondria to recipient BECs in culture and neurons mice acute cortical hippocampal slices. This mitochondrial increased ATP levels by 100 200-fold (relative untreated cells) exposed oxygen-glucose deprivation, an vitro model cerebral ischemia. also demonstrated larger EV fraction, but not exosomes...