A5034057755- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Sepsis Diagnosis and Treatment
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Respiratory Support and Mechanisms
- Pulmonary Hypertension Research and Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Proteoglycans and glycosaminoglycans research
- Neonatal Respiratory Health Research
- Hemoglobin structure and function
- Cardiac Arrest and Resuscitation
- Cardiovascular and Diving-Related Complications
- Antimicrobial Resistance in Staphylococcus
- Photopolymerization techniques and applications
- Polymer Nanocomposite Synthesis and Irradiation
- Thermal and Kinetic Analysis
- Blood transfusion and management
- Pharmacological Receptor Mechanisms and Effects
- Polymer crystallization and properties
- Streptococcal Infections and Treatments
- Synthesis and characterization of novel inorganic/organometallic compounds
- Advanced Optical Imaging Technologies
- High Altitude and Hypoxia
- Material Properties and Applications
- Neuropeptides and Animal Physiology
Massachusetts General Hospital
2023-2024
Harvard University
2023-2024
University of Colorado Denver
2017-2023
University of Colorado Anschutz Medical Campus
2021-2023
Pulmonary and Critical Care Associates
2021
Pharmac
2020
University of South Alabama
2013-2019
Tokyo University of Science
2018
Kyushu University
2011
The University of Tokyo
1967-1998
Septic patients frequently develop cognitive impairment that persists beyond hospital discharge. The impact of sepsis on electrophysiological and molecular determinants learning is underexplored. We observed mice survived or endotoxemia experienced loss hippocampal long-term potentiation (LTP), a brain-derived neurotrophic factor-mediated (BDNF-mediated) process responsible for spatial memory formation. Memory occurred despite preserved BDNF content could be reversed by stimulation...
We have investigated the temporal relationship between hemodynamic and histological/morphological progression in a rat model of pulmonary arterial hypertension that develops lesions morphologically indistinguishable from those human hypertension. Adult male rats were injected with Sugen5416 exposed to hypoxia for 3 wk followed by return normoxia various additional weeks. At 1, 3, 5, 8, 13 after injection, histological examinations performed. Right ventricular systolic pressure reached its...
Acute respiratory distress syndrome (ARDS) is a common cause of failure yet has few pharmacologic therapies, reflecting the mechanistic heterogeneity lung injury. We hypothesized that damage to alveolar epithelial glycocalyx, layer glycosaminoglycans interposed between epithelium and surfactant, contributes injury in patients with ARDS. Using mass spectrometry airspace fluid noninvasively collected from mechanically ventilated patients, we found glycosaminoglycan shedding (an index...
The lung epithelial glycocalyx is a carbohydrate-enriched layer lining the pulmonary surface. Although visualization has been reported, its composition and function remain unknown. Using immunofluorescence mass spectrometry, we identified heparan sulfate (HS) chondroitin within glycocalyx. In vivo selective enzymatic degradation of HS, but not sulfate, increased permeability. spectrometry gel electrophoresis approaches to determine fate HS during injury, detected shedding 20 saccharide-long...
Sepsis induces heparanase-mediated degradation of the endothelial glycocalyx, a heparan sulfate-enriched endovascular layer critical to vascular homeostasis, releasing highly sulfated domains sulfate into circulation. These are oligosaccharides rich in heparin-like trisulfated disaccharide repeating units. Using chemoenzymatic approach, an undecasaccharide containing uniformly
The pulmonary epithelial glycocalyx, an anionic cell surface layer enriched in glycosaminoglycans such as heparan sulfate and chondroitin sulfate, contributes to the alveolar barrier. Direct injury epithelium induces shedding of into air space; impact this on recovery after lung is unknown. Using mass spectrometry, we found that was shed space for up 3 wk intratracheal bleomycin-induced coincided with induction matrix metalloproteinases (MMPs), including MMP2. Delayed inhibition...
Acute respiratory distress syndrome (ARDS) is often seen in patients resuscitated from out-of-hospital cardiac arrest (OHCA). We aim to test whether inflammatory or endothelial injury markers are associated with the development of ARDS hospitalized after OHCA. conducted a prospective, cohort, pilot study at an urban academic medical center 2019 that included convenience sample adults non-traumatic Blood and pulmonary edema fluid (PEF) were collected within 12 hours hospital arrival. Samples...
Abstract Radiation‐induced polymerization of acetylene was investigated in the liquid and solid states monomer. It concluded from these experimental results that phase proceeds by a cationic mechanism not radical or ionic mechanism, an ordinary sense. The structure polymer obtained state is quite different, is, former mainly cis ‐form polyacetylene latter trans‐form one. These facts confirm stereospecific possible polymerization, using ionizing radiation, as previously pointed out authors....
Minimal change disease (MCD) is considered a podocyte disorder triggered by unknown circulating factors. Here, we hypothesized that the endothelial cell (EC) also involved in MCD.
ABSTRACT Staphylococcus aureus is one of the leading causes hospital-acquired infections, many which begin following attachment and accumulation on indwelling medical devices or diseased tissue. These infections are often linked to establishment biofilms, but another overlooked key characteristic allowing S. establish persistent infection formation planktonic aggregates. Such aggregates physiologically similar biofilms protect pathogens from innate immune clearance increase antibiotic...
Advances in tissue fixation and imaging techniques have yielded increasing appreciation for the glycosaminoglycan‐rich endothelial glycocalyx its vivo manifestation, surface layer (ESL). Pathological loss of ESL during critical illness promotes local dysfunction and, consequently, organ injury. Glycosaminoglycan fragments, such as heparan sulfate, are released into plasma animals humans after degradation thus served a biomarker The development state‐of‐the‐art glycomic techniques, however,...
Sepsis patients are at increased risk for hospital-acquired pulmonary infections, potentially due to postseptic immunosuppression known as the compensatory anti-inflammatory response syndrome (CARS). CARS has been attributed leukocyte dysfunction, with an unclear role endothelial cells. The circulation is lined by glycocalyx, a heparan sulfate-rich layer essential homeostasis. Heparan sulfate degradation occurs early in sepsis, leading lung injury. Endothelial synthesis of new sulfates...
Acute respiratory distress syndrome (ARDS) remains a significant cause of morbidity and mortality in critically ill patients. Oxidative stress inflammation play crucial role the pathogenesis ARDS. Extracellular superoxide dismutase (EC-SOD) is abundant lung an important enzymatic defense against superoxide. Human single-nucleotide polymorphism matrix binding region EC-SOD leads to substitution arginine glycine at position 213 (R213G) results release into alveolar fluid, without affecting...
Background Sepsis-associated destruction of the pulmonary microvascular endothelial glycocalyx (EGCX) creates a vulnerable surface, contributing to development acute respiratory distress syndrome (ARDS). Constituents EGCX shed into circulation, glycosaminoglycans and proteoglycans, may serve as biomarkers dysfunction. We sought define patterns plasma degradation products in children with sepsis-associated pediatric ARDS (PARDS), test their association clinical outcomes. Methods...
Epoxyeicosatrienoicacids (EETs), synthesized from arachidonic acid by epoxygenases of the CYP2C and CYP2J gene subfamilies, contribute to hypoxic pulmonary vasoconstriction (HPV) in mice. Despite their roles HPV, it is controversial whether EETs mediate or ameliorate hypertension (PH). A recent study showed that deficiency Cyp2j did not protect male female mice hypoxia-induced PH. Since CYP2C44 a functionally important epoxygenase, we hypothesized knockout Cyp2c44 would both sexes We tested...
Bacterial pneumonia is a common clinical syndrome leading to significant morbidity and mortality worldwide. In the current study, we investigate novel, multidirectional relationship between pulmonary epithelial glycocalyx antimicrobial peptides in setting of methicillin-resistant
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPolymerization and Dimerization of Vinylcarbazole. II. Relation between Reactive Intermediates Final Products in Irradiated Benzonitrile SolutionsS. Tagawa, S. Arai, M. Imamura, Y. Tabata, K. OshimaCite this: Macromolecules 1974, 7, 3, 262–264Publication Date (Print):May 1, 1974Publication History Published online1 May 2002Published inissue 1 1974https://pubs.acs.org/doi/10.1021/ma60039a002https://doi.org/10.1021/ma60039a002research-articleACS...
Histological observations in human pulmonary arterial hypertension (PAH) suggest a link between plexiform lesions and supernumerary arteries. Pulmonary microvascular endothelial cells are characterized as hyperproliferative progenitor-like. This study investigates the hypothesis that aneurysm-type form arteries because of their anatomical properties characteristics similar to cells. To induce PAH, rats were injected with Sugen5416, exposed hypoxia (10% O 2 ) for 3 days (early stage) or wk...
Chronic thromboembolic pulmonary hypertension (CTEPH) is a hot topic in the field of hypertension, because many CTEPH patients are now curable by surgical endarterectomy and more recently possibly balloon angioplasty. However, there still uncertainties regarding pathogenesis CTEPH, specifically how where small vessel arteriopathy that indistinguishable from arterial (plexogenic arteriopathy) develops, improves hemodynamics cures CTEPH. Based on our recent experimental finding hemodynamic...