A5011324742- Sepsis Diagnosis and Treatment
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Gut microbiota and health
- Acute Kidney Injury Research
- Dialysis and Renal Disease Management
- Chronic Kidney Disease and Diabetes
- Clostridium difficile and Clostridium perfringens research
- Traumatic Brain Injury and Neurovascular Disturbances
- Neuroscience of respiration and sleep
- Immune Response and Inflammation
- Diet and metabolism studies
- COVID-19 Clinical Research Studies
- Psoriasis: Treatment and Pathogenesis
- Intensive Care Unit Cognitive Disorders
- Proteoglycans and glycosaminoglycans research
- Erythropoietin and Anemia Treatment
- Hemoglobin structure and function
- Inflammatory Bowel Disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Botulinum Toxin and Related Neurological Disorders
- Helicobacter pylori-related gastroenterology studies
- Advanced Drug Delivery Systems
- Acute Ischemic Stroke Management
- Urinary Tract Infections Management
- Long-Term Effects of COVID-19
University of Colorado Anschutz Medical Campus
2017-2025
University of Colorado Denver
2016-2024
The Medical Center of Aurora
2023
Advisory Board Company (United States)
2015-2017
Center for Health Care Law
2016-2017
Oregon Health & Science University
2013
Intravenous fluids, an essential component of sepsis resuscitation, may paradoxically worsen outcomes by exacerbating endothelial injury. Preclinical models suggest that fluid resuscitation degrades the glycocalyx, a heparan sulfate-enriched structure necessary for vascular homeostasis. We hypothesized glycocalyx degradation is associated with volume intravenous fluids administered during early resuscitation.We used mass spectrometry to measure plasma sulfate (a highly sensitive and specific...
The endothelial glycocalyx is a heparan sulfate (HS)-rich endovascular structure critical to function. Accordingly, degradation during sepsis contributes tissue edema and organ injury. We determined the endogenous mechanisms governing pulmonary reconstitution, if these reparative are impaired sepsis. performed intravital microscopy of wild-type transgenic mice determine rapidity reconstitution after nonseptic (heparinase-III mediated) or septic (cecal ligation puncture degradation. used mass...
<title>Abstract</title> Background Procalcitonin is a 14.5 kDa protein used clinically as marker of sepsis and therapeutic response to antibiotic therapy. However, its utility in critically ill patients with either acute kidney injury (AKI) or end-stage disease (ESKD) who require continuous replacement therapy (CKRT) unknown. The aim this study was determine if plasma levels procalcitonin could reliably distinguish septic from nonseptic status AKI ESKD prior during CKRT. Methods...
Sepsis outcomes are heavily dependent on the development of septic organ injury, but no interventions exist to interrupt or reverse this process. microRNA-223 (miR-223) is known be involved in both inflammatory gene regulation and host-pathogen interactions key pathogenesis sepsis. The goal study was determine role miR-223 as a mediator kidney injury. Using knockout mice multiple models experimental sepsis, we found that differentially influences acute injury (AKI) based model used. In...
Older adults suffer more frequent and worse outcomes from sepsis, a critical illness secondary to infection. The reasons underlying this unique susceptibility are incompletely understood.
Patients with inflammatory bowel disease have a unique response to infection given the pathogenesis of these diseases and common use immunosuppressive therapy.The goal this study is determine severe sepsis outcomes in subgroup visits comorbidities disease.The 2012 National Inpatient Sample database was used identify patients explicitly coded diagnoses or septic shock. Visits chronic other were identified using ICD-9 codes. Sepsis interest codes.There 92,296 for shock analysis. In control...
Key Points Acute kidney disease (AKD) and CKD are common conditions associated with high rates of incident infection, poor outcomes once infection have been established. We successfully modeled AKD in rodents then administered a cecal slurry solution to create peritonitis tracked sepsis severity, end organ injury, inflammatory changes. Our results indicate that mice more susceptible than mice, developing an aggravated response suggests this condition predisposes disparate risk. Background...
Severe acute kidney injury (AKI) is associated with subsequent infection. Whether AKI followed by a return to baseline creatinine incident infection unknown.We hypothesized that risk of both short and long term would be higher among patients than in propensity score matched peers without the year following non-infectious hospital admission.Retrospective, cohort study.We identified 494 who were hospitalized between January 1, 1999 December 31, 2009 had creatinine. These controls AKI.The...
The vagus nerve contains primary visceral afferents that convey sensory information from cardiovascular, pulmonary, and gastrointestinal tissues to the nucleus tractus solitarii (NTS). heterogeneity of vagal their central terminals within NTS is a common obstacle for evaluating functional groups afferents. To determine whether different anterograde tracers can be used identify distinct subpopulations NTS, we injected cholera toxin B subunit (CTb) isolectin B4 (IB4) into nerve. Confocal...
Bacterial pneumonia is a common clinical syndrome leading to significant morbidity and mortality worldwide. In the current study, we investigate novel, multidirectional relationship between pulmonary epithelial glycocalyx antimicrobial peptides in setting of methicillin-resistant
Key Points Plasma cystatin C is decreased in participants with AKI requiring continuous KRT (CKRT) early kidney function recovery. Despite being cleared by CKRT, plasma the first 3 days of CKRT may be a useful clinical tool to help predict prognosis CKRT. Background reliable marker estimate function; however, it unknown whether this remains true patients receiving (CKRT). In study, we tested hypothesis that lower concentrations during would Methods We performed retrospective observational...
Patients with acute kidney injury (AKI) or end stage disease (ESKD) may require continuous renal replacement therapy (CRRT) as a supportive intervention. While CRRT is effective at achieving solute control and fluid balance, the indiscriminate nature of this procedure raises possibility that beneficial substances similarly be removed. Hepcidin, an antimicrobial peptide pivotal roles in iron homeostasis pathogen clearance, has biochemical properties amenable to direct removal via CRRT. We...
Prior research has focused on host factors as mediators of exaggerated sepsis-associated morbidity and mortality in older adults. This focus the host, however, failed to identify therapies that improve sepsis outcomes elderly. We hypothesized increased susceptibility aging population is not only a function but also reflects longevity-associated changes virulence gut pathobionts. utilized two complementary models microbiota-induced experimental establish aged microbiome key pathophysiologic...
BACKGROUND Sepsis, a dysregulated systemic response to infection, is major cause of morbidity and mortality worldwide. Older adults are particularly vulnerable this syndrome, as evidenced by higher sepsis incidence mortality. It well established that the development organ failure, specifically acute kidney injury (AKI), strong predictor during occurs more frequently in aging population. Despite association, mechanisms underlying septic AKI remain undetermined. Extensive prior research...
The vagus nerve contains primary visceral afferents that convey information from cardiovascular, pulmonary, and gastrointestinal tissues to nucleus tractus solitarii (NTS). We tested the hypothesis different anterograde tracers will label distinct populations of afferents. In somatic nerves, cholera toxin B subunit (CTb) isolectin B4 (IB4) have been shown myelinated unmyelinated fibers, respectively. also examined vesicular glutamate transporter 2 (VGlut2) content these Male Sprague-Dawley...
Background The alveolar airspace is lined by a heparan sulfate (HS)-enriched epithelial glycocalyx. We previously demonstrated that direct lung injury (i.e., caused inhalational insults) causes shedding of HS into the airspaces mice and humans. However, homeostatic function surface uncertain. hypothesized during homeostasis, contributes to aqueous hypophase upon which surfactant distributed. As such, experimental degradation should lead collapse loss compliance, key characteristics ARDS...