A5021295192- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Vector-Borne Animal Diseases
- Fire effects on ecosystems
- Mosquito-borne diseases and control
- Monoclonal and Polyclonal Antibodies Research
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Virus Research Studies
- Influenza Virus Research Studies
- Rabies epidemiology and control
- Animal Virus Infections Studies
- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- Virology and Viral Diseases
- Animal Disease Management and Epidemiology
- interferon and immune responses
- Transgenic Plants and Applications
- Bacillus and Francisella bacterial research
- Virus-based gene therapy research
- Protein purification and stability
- COVID-19 Clinical Research Studies
- Viral Infections and Immunology Research
National Institute for Viral Disease Control and Prevention
2016-2025
Chinese Center For Disease Control and Prevention
2015-2024
Renji Hospital
2024
Chinese Academy of Sciences
2020-2022
Wuhan Center for Disease Control and Prevention
2022
National Health and Family Planning Commission
2016-2019
Center for Disease Control
2007-2018
Nanchang Center for Disease Control and Prevention
2016
Istituto Superiore di Sanità
2016
University of Sassari
2016
Ebola virus has been detected in the semen of men after their recovery from disease (EVD). We report presence RNA a cohort survivors EVD Sierra Leone.We enrolled convenience sample 220 adult male Leone, at various times discharge an treatment unit (ETU), two phases (100 participants were phase 1, and 120 2). Semen specimens obtained baseline tested by means quantitative reverse-transcriptase-polymerase-chain-reaction (RT-PCR) assay with use target sequences NP VP40 (in 1) or GP This study...
Background. Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by the SFTS virus (SFTSV) average fatality rate of 12%. The clinical factors for death in patients remain unclear.
Abstract To investigate the infections of severe fever with thrombocytopenia syndrome virus (SFTSV) in domesticated animals, we sampled a total 3,039 animals 2 counties Shandong Province, People's Republic China, from April to November 2011. SFTSV-specific antibodies were detected 328 (69.5%) 472 sheep, 509 (60.5%) 842 cattle, 136 (37.9%) 359 dogs, 26 (3.1%) 839 pigs, and 250 (47.4%) 527 chickens. SFTSV RNA was all animal species, but prevalence low, ranging 1.7% 5.3%. A cohort study 38...
The discovery of an emerging viral disease, severe fever with thrombocytopenia syndrome (SFTS), caused by SFTS virus (SFTSV), has prompted the need to understand pathogenesis SFTSV. We are unique in establishing infectious model C57/BL6 mice, resulting hallmark symptoms and leukocytopenia. Viral RNA histopathological changes were identified spleen, liver, kidney. However, replication was only found which suggested spleen be principle target organ Moreover, number macrophages platelets...
Zika virus–specific antibodies isolated from a single infected patient show postexposure protection in mice and reveal targets for therapy.
An outbreak of coronavirus disease (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) poses great threats to human health and the international economy. To reduce large-scale infection transmission risk SARS-CoV-2, a simple, rapid, sensitive serological diagnostic method is urgently needed. Herein, an aggregation-induced emission (AIE) nanoparticle (AIE810NP, λem = 810 nm)-labeled lateral flow immunoassay was designed for early detection immunoglobulin M (IgM) G (IgG)...
Significance Bunyaviruses are emerging zoonotic pathogens of public-health concern. Lack structures for proteins on the viral membrane (“envelope”) surface limits understanding entry. We describe atomic-level globular “head” envelope protein, glycoprotein N (Gn), from two members, severe fever with thrombocytopenia syndrome virus (SFTSV) and Rift Valley (RVFV), Phleboviruses genus in bunyavirus family, a structure SFTSV Gn bound neutralizing antibody Fab. The results show folded define...
ABSTRACT Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease characterized by high fever, thrombocytopenia, multiorgan dysfunction, and a fatality rate between 12 30%. It caused SFTS virus (SFTSV), novel Phlebovirus in family Bunyaviridae . Although the viral pathogenesis remains largely unknown, hemopoietic cells appear to be targeted virus. In this study we report that human monocytes were susceptible SFTSV, which replicated efficiently, as shown...
Hemorrhagic fever with renal syndrome (HFRS) caused by hantaviruses is a serious public health problem in China. The National Notifiable Disease Surveillance System (NNDSS) was established online China CDC 2004 and rodent surveillance sites were adjusted to 40 22 provinces 2005. Here we analyzed the data of both human cases rodents host during 2006-2012 examine epidemic trends HFRS recent years China.Records on from 2006 2012 analyzed. Phylogenetic tree based complete sequence M segment 58...
THP-1 monocyte, which can be differentiated into macrophages by PMA, is widely used in researches on pathogen infection and host innate immunity, but reports the induction methods of PMA are different lack a unified standard, transcriptome characteristics macrophage compared with cells remains unclear. In this research, we examined differentiation effect three factors including time, cell seeding density concentration detecting positive rate CD14 expression. The 80ng/ml time 24h, 5×10 5...
Production of high-value recombinant proteins in transgenic seeds is an attractive and economically feasible alternative to conventional systems based on mammalian cells bacteria. In contrast leaves, allow high-level accumulation a relatively small volume stable environment. We demonstrate that single-chain variable fragment (scFv)-Fc antibodies, with N-terminal signal sequence C-terminal KDEL tag, can accumulate very high levels as bivalent IgG-like antibodies Arabidopsis thaliana...
ABSTRACT Cells are equipped with pattern recognition receptors (PRRs) such as the Toll-like and RIG-I-like that mount innate defenses against viruses. However, viruses have evolved multiple strategies to evade or thwart host antiviral responses. Viral inclusion bodies (IBs), which accumulated aggregates of viral proteins, commonly formed during replication some in infected cells, but their role immune evasion has rarely been explored. Severe fever thrombocytopenia syndrome (SFTS) is an...
Background Severe Fever with Thrombocytopenia Syndrome (SFTS) is an emerging infectious disease in East Asia. SFTS a tick borne hemorrhagic fever caused by SFTSV, new bunyavirus named after the syndrome. We investigated epidemiology of Laizhou County, Shandong Province, China. Methods collected serum specimens all patients who were clinically diagnosed as suspected cases 2010 and 2011 County. The patients' tested for SFTSV real time fluorescence quantitative PCR (RT-qPCR). 1,060 from healthy...
ABSTRACT Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne pathogen that was first reported in China 2009. Phylogenetic analysis of the viral genome showed SFTS represents a new lineage within Phlebovirus genus, distinct from existing sandfly and Uukuniemi groups, family Bunyaviridae . disease characterized by gastrointestinal symptoms, chills, joint pain, myalgia, thrombocytopenia, leukocytopenia, some hemorrhagic manifestations case fatality rate about 2...
The pathogenesis of clinical manifestations caused by newly emerging tick-borne phleboviruses [i.e., Severe fever with thrombocytopenia syndrome virus (SFTSV) and Heartland (HRTV)], such as severe lymphocytopenia, are not yet fully understood. In the present study, to establish an animal model mimicking profile fatal human cases, we examined susceptibilities adult mice from 12 strains, aged two cynomolgus macaques SFTSV and/or HRTV infections. However, none these immunocompetent animals...
Severe fever with thrombocytopenia syndrome is an emerging infectious disease caused by a novel bunyavirus (SFTSV). Lack of vaccines and inadequate therapeutic treatments have made the spread virus global concern. Viral nucleocapsid protein (N) essential for its transcription replication. Here, we present crystal structures N from SFTSV homologs Buenaventura (BUE) Granada (GRA) viruses. The reveal that phleboviral folds into compact core domain extended N-terminal arm mediates...
In total, 3,145 ticks of the species Haemaphysalis longicornis (3,048; 96.9%), R. microplus (82; 2.6%), H. campanulata (9; 0.3%), and Dermacentor sinicus (5; 0.2%) were collected from animals vegetation at Yantai in Shandong Province. Both adult immature obtained, all unfed. Eggs obtained 22 blood-fed female through maintenance room temperature after collection. Severe fever with thrombocytopenia syndrome virus (SFTSV) viral RNA was identified microplus, a prevalence 4.75 per 100 (95%...
Severe fever with thrombocytopenia syndrome virus (SFTSV) is a new tick-borne pathogen that can cause severe hemorrhagic fever. Fever caused by SFTSV infectious disease has posed great threat to public health. Therefore, fast, sensitive, low-cost, and field-deployable detection method for diagnosing essential surveillance control. In this study, we developed rapid, highly instrument-flexible utilizes recombinase polymerase amplification the CRISPR/Cas12a system. We found three copies of L...
The development of new therapeutic targets and strategies to control highly pathogenic avian influenza (HPAI) H5N1 virus infection in humans is urgently needed. Broadly cross-neutralizing recombinant human antibodies obtained from the survivors provide an important role immunotherapy for definition critical epitopes vaccine development.We have characterized two baculovirus-expressed (rhAbs), AVFluIgG01 AVFluIgG03, generated by screening a Fab antibody phage library derived patient recovered...
Severe fever with thrombocytopenia syndrome (SFTS) virus is an emerging bunyavirus that causes a hemorrhagic high mortality rate. The likely tick-borne and replicates primarily in hemopoietic cells, which may lead to disregulation of proinflammatory cytokine induction loss leukocytes platelets. viral genome contains L, M, S segments encoding RNA polymerase, glycoproteins Gn Gc, nucleoprotein (NP), nonstructural segment (NSs) protein. NSs protein involved the regulation host innate immune...
Background Viral hemorrhagic fevers (VHFs) are a group of animal and human illnesses that mostly caused by several distinct families viruses including bunyaviruses, flaviviruses, filoviruses arenaviruses. Although specific signs symptoms vary the type VHF, initial very similar. Therefore rapid immunologic molecular tools for differential diagnosis fever (HFVs) important effective case management control spread VHFs. Real-time quantitative reverse transcriptase-polymerase chain reaction...
SFTS virus (SFTSV) is a highly pathogenic bunyavirus that causes severe fever with thrombocytopenia syndrome (SFTS), an emerging infectious disease in China. Laboratory mice have been reported to be susceptible SFTSV infection, but the infection nonhuman primates has not investigated. This study first report that, rhesus macaques, does cause symptoms or death fever, thrombocytopenia, leukocytopenia, and increased levels of transaminases myocardial enzymes blood. Viremia, virus-specific...