A5046727928- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Toxin Mechanisms and Immunotoxins
- Monoclonal and Polyclonal Antibodies Research
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Genetic and Mutation Studies
- Genetically Modified Organisms Research
- Animal Genetics and Reproduction
- Legume Nitrogen Fixing Symbiosis
- Genetic Mapping and Diversity in Plants and Animals
- Wheat and Barley Genetics and Pathology
- Viral gastroenteritis research and epidemiology
- Plant-Microbe Interactions and Immunity
- Escherichia coli research studies
- Glycosylation and Glycoproteins Research
- Plant Disease Resistance and Genetics
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Photosynthetic Processes and Mechanisms
- Agriculture, Soil, Plant Science
- Genetic and phenotypic traits in livestock
VIB-UGent Center for Plant Systems Biology
2010-2020
Ghent University
2011-2020
Vlaams Instituut voor Biotechnologie
2001-2013
Ghent University Hospital
2008-2013
GTx (United States)
2000-2013
BOKU University
2011
Czech Academy of Sciences, Institute of Biophysics
2003-2009
Institute of Genetics
1991
University of Helsinki
1989
University of California, San Francisco
1980
Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has distinctive combination features--i.e., (i) silencing occurs by posttranscriptional process, (ii) correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located the symmetrical motifs CG CXG.
Nicotiana protoplasts and Arabidopsis leaf discs or roots were co-cultivated with two Agrobacterium strains each carrying a different T-DNA. Co-transformed plants selected the integration of T-DNAs was analysed at genetic genomic level. Genetic analysis showed that derived from bacteria frequently integrated same locus, independent plant species transformation method used. Southern revealed 12 out 27 transformants contained co-transferred linked to other in all possible configurations but...
Crown gall tumors are induced in plants by infection with the soil bacterium Agrobacterium tumefaciens. Because tumor induction involves transfer of a portion tumor-inducing (Ti) plasmid DNA from to plant cells, this system is interest for study genetic exchange as well induction. The boundaries transferred (T-DNA) have been cloned transformed cells tobacco. Detailed mapping restriction enzymes and nucleotide sequence analysis two independent clones were used molecular structure ends T-DNA....
Agrobacterium tumefaciens is a gram-negative bacterium with the unique capacity to induce neoplasmic transformations in dicotyledonous plants. Recently, both mechanism and biological significance of this transformation have been elucidated. strains contain large extrachromosomal DNA plasmid (the Ti-plasmid). This Ti-plasmid responsible for oncogenic properties strains. A particular segment Ti-plasmid, containing information determining tumorous growth pattern synthesis so-called 'opines',...
Oral feed-based passive immunization can be a promising strategy to prolong maternal lactogenic immunity against postweaning infections. Enterotoxigenic Escherichia coli (ETEC)-caused diarrhea in piglets is one such infection that may prevented by oral and might avert recurrent economic losses the pig farming industry. As proof of principle, we designed anti-ETEC antibodies fusing variable domains llama heavy chain-only (VHHs) ETEC Fc part porcine immunoglobulin (IgG or IgA) expressed them...
After Agrobacterium-mediated plant transformation, multiple T-DNAs frequently integrate at the same position in genome, resulting formation of inverted and direct repeats. Because these repeats cannot be amplified analyzed by PCR, Arabidopsis root cells were co-transformed with two different distinct sequences adjacent to T-DNA borders. Nine or border junctions sequence level. Precise end-to-end fusions found between right ends, whereas imprecise filler DNA present linkages containing a left...