A5035569738- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Glycosylation and Glycoproteins Research
- Plant tissue culture and regeneration
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Transgenic Plants and Applications
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Carbohydrate Chemistry and Synthesis
- Chromosomal and Genetic Variations
- Legume Nitrogen Fixing Symbiosis
- Plant Genetic and Mutation Studies
- Probiotics and Fermented Foods
- Cell Adhesion Molecules Research
- Toxin Mechanisms and Immunotoxins
- Antimicrobial Resistance in Staphylococcus
- CRISPR and Genetic Engineering
- Bacterial biofilms and quorum sensing
- Enzyme Structure and Function
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Galectins and Cancer Biology
- Plant Reproductive Biology
- Salmonella and Campylobacter epidemiology
Vrije Universiteit Brussel
2016-2025
Vlaams Instituut voor Biotechnologie
2003-2023
VIB-VUB Center for Structural Biology
2011-2022
VIB-UGent Center for Plant Systems Biology
2018
KU Leuven
2009
European Food Safety Authority
2009
Instituut voor Tropische Geneeskunde
2009
In-Q-Tel
2004
Veterinary and Agrochemical Research Centre
1996
University of Zurich
1996
Journal Article Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants Get access R. Deblaere, Deblaere Laboratorium voor Genetische Virologie, Vrije Universiteit BrusselB-1640 St.-Genesius-Rode, Belgium Search other works by this author on: Oxford Academic PubMed Google Scholar B. Bytebier, Bytebier H. De Greve, Greve F. Deboeck, Deboeck J. Schell, Schell M. Van Montagu, Montagu Leemans Nucleic Acids Research, Volume 13, Issue 11 July 1985, Pages...
Summary Mannose‐binding type 1 pili are important virulence factors for the establishment of Escherichia coli urinary tract infections (UTIs). These initiated by adhesion uropathogenic E. to uroplakin receptors in uroepithelium via FimH adhesin located at tips pili. Blocking bacterial is able prevent infection. Here, we provide first time binding data molecular events underlying fimbrial adherence, crystallographic analyses receptor domains from a and K‐12 strain, affinity measurements with...
Escherichia coli strains adhere to the normally sterile human uroepithelium using type 1 pili, that are long, hairy surface organelles exposing a mannose-binding FimH adhesin at tip. A small percentage of adhered bacteria can successfully invade bladder cells, presumably via pathways mediated by high-mannosylated uroplakin-Ia and alpha3beta1 integrins found throughout uroepithelium. Invaded replicate mature into dense, biofilm-like inclusions in preparation fluxing infection neighbouring...
A plasmid-encoded crystal protein gene (bt2) has been cloned from Bacillus thuringiensis berliner 1715. In Escherichia coli, it directs the synthesis of 130-kDa (Bt2) which is toxic to larvae Pieris brassicae and Manduca sexta. Comparison deduced amino acid sequence this Bt2 with B. kurstaki HD1 Dipel, HD73 sotto sequences suggests that homologous recombination between different genes occurred during evolution. Treatment trypsin or chymotrypsin yields a 60-kDa protease-resistant fully...
A procedure is presented, that has allowed the rapid assignment of transposon Tn1 and Tn7 insertion sites in large (130 Md) nopaline Ti-plasmid pTiC58, to specific restriction enzyme fragments. Total bacterial DNA isolated from Agrobacterium tumefaciens strain C58 mutants carry a their Ti-plasmid, digested with an appropriate endonuclease. The fragments are separated on agarose gel, denatured transferred nitrocellulose filters. These hybridized against purified wild type or segments PTiC58,...
Oral feed-based passive immunization can be a promising strategy to prolong maternal lactogenic immunity against postweaning infections. Enterotoxigenic Escherichia coli (ETEC)-caused diarrhea in piglets is one such infection that may prevented by oral and might avert recurrent economic losses the pig farming industry. As proof of principle, we designed anti-ETEC antibodies fusing variable domains llama heavy chain-only (VHHs) ETEC Fc part porcine immunoglobulin (IgG or IgA) expressed them...
Summary Type‐1 fimbriae are important virulence factors for the establishment of Escherichia coli urinary tract infections. Bacterial adhesion to high‐mannosylated uroplakin Ia glycoprotein receptors bladder epithelium is mediated by FimH adhesin. Previous studies have attributed differences in mannose‐sensitive phenotypes between faecal and uropathogenic E. sequence variation receptor‐binding domain. We find that variants from uropathogenic, enterohaemorrhagic isolates express same...
Vectors were constructed for the isolation of random transcriptional and translational beta-glucuronidase gene fusions in plants. This system is based on integration transferred DNA (T-DNA) into plant nuclear genome. The Escherichia coli coding sequence without promoter, also devoid its ATG initiation site fusion vector, was inserted T-DNA with 5' end at a distance 4 base pairs from right border sequence. Transgenic plants can be selected by using chimeric (P35S-nptII-3' ocs)...
Asparagus officinalis was the first monocotyledonous plant from which hormone-independent and opine-producing crown gall tissue could be isolated. We confirm by DNA hybridization that tumor lines obtained after infection of this Agrobacterium strains harboring wild-type nopaline octopine tumor-inducing (Ti) plasmids are stably transformed contain transferred (T-DNA) segments identical to T-DNA found in dicotyledonous plants. have also infected with a nononcogenic vector carries chimeric...
F107 fimbriae were isolated and purified from edema disease strain 107/86 of Escherichia coli. Plasmid pIH120 was constructed, which contains the gene cluster that codes for adhesive fimbriae. The major fimbrial subunit gene, fedA, sequenced. An open reading frame a protein with 170 amino acids, including 21-amino-acid signal peptide, found. without sequence has calculated molecular mass 15,099 Da. Construction nonsense mutation in fedA abolished both expression capacity to adhere porcine...
Summary To cope with iron deficiency fluorescent pseu‐domonads produce pyoverdines which are complex peptidic siderophores that very efficiently scavenge iron. In addition to pyoverdine some species also other siderophores. Recently, it was shown Pseudomonas fluorescens ATCC 17400 pro‐duces the siderophore quinolobactin, an 8‐hydroxy‐4‐methoxy‐2‐quinoline carboxylic acid (Mossialos, D., Meyer, J.M., Budzikiewicz, H., Wolff, U., Koedam, N., Baysse, C., Anjaiah, V., and Cornelis, P. (2000)...