- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Zoonotic diseases and public health
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Food Safety and Hygiene
- Vibrio bacteria research studies
- Clostridium difficile and Clostridium perfringens research
- Microbial infections and disease research
- Veterinary medicine and infectious diseases
- Pharmaceutical and Antibiotic Environmental Impacts
- Vector-Borne Animal Diseases
- Animal Nutrition and Physiology
- Antibiotics Pharmacokinetics and Efficacy
- Listeria monocytogenes in Food Safety
- Probiotics and Fermented Foods
- Nutrition and Health Studies
- Molecular Biology Techniques and Applications
- Plant and animal studies
- Cardiovascular and exercise physiology
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
Sciensano (Belgium)
2023-2024
Federal Agency for Food Chain Safety
2007-2023
Institut Scientifique de Santé Publique
2012-2023
Veterinary and Agrochemical Research Centre
2007-2017
Département Santé Animale
2009
Ghent University
2008
Koninklijke Nederlandse Maatschappij voor Diergeneeskunde
2004
National Institute of Veterinary Research
1993-2001
Economic Research Centre
2000
Vrije Universiteit Brussel
1989-1996
Salmonella enterica serovar Typhimurium phage type DT204 strains isolated from cattle and animal feed in Belgium were characterized for high-level fluoroquinolone resistance mechanisms [MICs to enrofloxacin (Enr) ciprofloxacin (Cip), 64 32 μg/ml, respectively]. These during the periods 1991-1994, 2000 clonally related as shown by pulsed-field gel electrophoresis (PFGE). Selected studied carried several mutations quinolone target genes, i.e., a double mutation resistance-determining region...
ABSTRACT Antibiotic treatment is not required in cases of Salmonella enterica gastroenteritis but essential enteric fever or invasive salmonellosis immunocompromised patients. Although fluoroquinolones and extended-spectrum cephalosporins are the drugs choice to treat , resistance these antibiotics increasing worldwide. During period 2000 2003, 90 serovar Virchow poultry product isolates 11 human were found produce an β-lactamase, CTX-M-2, concomitantly with a TEM-1 β-lactamase. The bla...
We report here the dissemination of a conjugative IncI1 plasmid carrying bla(TEM-52) on Tn3 transposon conferring resistance to extended-spectrum cephalosporins in Salmonella enterica serovar Agona, Derby, Infantis, Paratyphi B dT(+), and Typhimurium isolates from poultry humans Belgium France 2001 2005. The most prevalent spreading this was Infantis.
To prioritize an extended list of food- and water-borne zoonoses to allow food safety authorities focus on the most relevant hazards in chain.An evidence-based semiquantitative methodology was developed. Scores were given by 35 scientific experts field animal public health, food, clinical microbiology epidemiology 51 zoonotic agents according five criteria related health (severity occurrence humans), disease coupled with economic consequences animals), (occurrence food). The scoring...
F107 fimbriae were isolated and purified from edema disease strain 107/86 of Escherichia coli. Plasmid pIH120 was constructed, which contains the gene cluster that codes for adhesive fimbriae. The major fimbrial subunit gene, fedA, sequenced. An open reading frame a protein with 170 amino acids, including 21-amino-acid signal peptide, found. without sequence has calculated molecular mass 15,099 Da. Construction nonsense mutation in fedA abolished both expression capacity to adhere porcine...
ABSTRACT Five repetitive-element PCR (rep-PCR) techniques [primer sets ERIC1R-ERIC2 and REP1R-REP2I primers ERIC2, BOXA1R, (GTG) 5 ] were evaluated for the discrimination of Salmonella enterica isolates at serotype level. On basis number, even distribution over whole fingerprint, clarity bands in fingerprints, enterobacterial repetitive intergenic consensus (ERIC) primer set chosen use following experiments. For these two sets, reproducibility was tested on different lysates five selected...
In Belgium, non-typhoidal salmonellosis and campylobacteriosis are the two most frequently reported foodborne illnesses. During 2005, a 71% decrease of Salmonella Enteritidis infections compared with average annual number cases in period 2000-2004 was recorded by Belgian National Reference Centre for Shigella. After peak 1999, total decreased gradually, exception 2003 when an increase again due to rise isolates belonging serotype Enteritidis. PT4, predominant phage type Enteriditis over...