A5089616388- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Escherichia coli research studies
- Cancer Research and Treatments
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial Genetics and Biotechnology
- Antibiotics Pharmacokinetics and Efficacy
- Enterobacteriaceae and Cronobacter Research
- Genomics and Phylogenetic Studies
- Drug Transport and Resistance Mechanisms
- Probiotics and Fermented Foods
- Bacterial biofilms and quorum sensing
- Bacterial Identification and Susceptibility Testing
- Cancer therapeutics and mechanisms
- Mycobacterium research and diagnosis
- Microbial Metabolism and Applications
- Animal Nutrition and Physiology
- Phenothiazines and Benzothiazines Synthesis and Activities
- CRISPR and Genetic Engineering
- Glycogen Storage Diseases and Myoclonus
- Brucella: diagnosis, epidemiology, treatment
- Silymarin and Mushroom Poisoning
- Identification and Quantification in Food
Université de Tours
2013-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Infectiologie Animale et Santé Publique
2015-2024
Institut National de la Recherche Agronomique
2003-2013
Institut Pasteur
2008-2009
Laboratoire National de Référence
2008
Biologie des Oiseaux et Aviculture
2001-2003
National Salmonella surveillance systems from France, England and Wales, Denmark, the United States identified recent emergence of multidrug-resistant isolates enterica serotype Kentucky displaying high-level resistance to ciprofloxacin. A total 489 human cases were during period 2002 (3 cases) 2008 (174 cases). These belonged a single clone defined by multilocus sequence type ST198, XbaI-pulsed-field gel electrophoresis cluster X1, presence genomic island 1 variant SGI1-K. This was probably...
Salmonella genomic island 1 (SGI1) is a containing an antibiotic resistance gene cluster identified in several enterica serovars. The SGI1 cluster, which complex class integron, confers the common multidrug phenotype of epidemic S. Typhimurium DT104. occurrence serovars Typhimurium, Agona, Paratyphi B, Albany, Meleagridis and Newport indicates horizontal transfer potential SGI1. Here, we report that could be conjugally transferred from donor strains to non-SGI1 Escherichia coli recipient...
While the spread of Salmonella enterica serotype Kentucky resistant to ciprofloxacin across Africa and Middle-East has been described recently, presence this strain in humans, food, various animal species (livestock, pets, wildlife) environment is suspected other countries different continents. Here, we report results an in-depth molecular epidemiological study on a global human non-human collection S. (n = 70). We performed XbaI-pulsed field gel electrophoresis multilocus sequence typing,...
Salmonella enterica serotype Kentucky can be a common causative agent of salmonellosis, usually associated with consumption contaminated poultry. Antimicrobial resistance (AMR) to multiple drugs, including ciprofloxacin, is an emerging problem within this serotype. We used whole-genome sequencing (WGS) investigate the phylogenetic structure and AMR content 121 S.enterica sequence type 198 isolates from five continents. Population was inferred using phylogenomic analysis whole genomes were...
Salmonella genomic island 1 (SGI1) contains an antibiotic resistance gene cluster and has been previously identified in multidrug-resistant enterica serovars Typhimurium DT104, Agona, Paratyphi B. We a variant SGI1 antibiotic-resistance strain of S. serovar Albany isolated from food fish Thailand imported to France. In this strain, the streptomycin aadA2 cassette one integrons was replaced by dfrA1 cassette, conferring trimethoprim open reading frame unknown function. Thus, represents fourth...
Background The Salmonella genomic island 1 (SGI1) is a enterica-derived integrative mobilizable element (IME) containing various complex multiple resistance integrons identified in several S. enterica serovars and Proteus mirabilis. Previous studies have shown that SGI1 transfers horizontally by trans mobilization the presence of IncA/C conjugative helper plasmid pR55. Methodology/Principal Findings Here, we report ability different prevalent multidrug (MDR) plasmids including...
We report here the dissemination of a conjugative IncI1 plasmid carrying bla(TEM-52) on Tn3 transposon conferring resistance to extended-spectrum cephalosporins in Salmonella enterica serovar Agona, Derby, Infantis, Paratyphi B dT(+), and Typhimurium isolates from poultry humans Belgium France 2001 2005. The most prevalent spreading this was Infantis.
A multiresistant Aeromonas bestiarum strain, shown to be persistent and spreading in a freshwater stream, was investigated for the presence, location organization of antimicrobial resistance genes. The plasmid pAB5S9 transferred by electroporation into Escherichia coli TG1. phenotype mediated determined. Moreover, sequenced completely analysed its structure reading frames. Plasmid resistances phenicols, sulphonamides, streptomycin tetracycline. analysis 24.7 kb sequence revealed presence 20...
Salmonella genomic island 1 (SGI1) is an integrative mobilizable element that harbors a multidrug resistance (MDR) gene cluster. Since its identification in epidemic enterica serovar Typhimurium DT104 strains, variant SGI1 MDR clusters conferring different phenotypes have been identified several S. serovars and classified as SGI1-A to -O. A study was undertaken characterize from Kentucky strains isolated travelers returning Africa. Several tested were found contain the partially...
Abstract The multidrug resistance Salmonella Genomic Island 1 (SGI1) is an integrative mobilizable element identified in several enterobacterial pathogens. This chromosomal island requires a conjugative IncA/C plasmid to be excised as circular extrachromosomal form and conjugally mobilized trans . Preliminary observations suggest stable maintenance of SGI1 the host chromosome but paradoxically also incompatibility between plasmids. Here, using enterica serovar Agona clonal bacterial...
ABSTRACT Salmonella genomic island 1 (SGI1) harbors an antibiotic resistance gene cluster and was previously identified in the multidrug-resistant enterica serovars Typhimurium DT104, Agona, Paratyphi B, Albany. This is a complex class integron most often confers to ampicillin (Ap), chloramphenicol (Cm)/florfenicol (Ff), streptomycin (Sm)/spectinomycin (Sp), sulfonamides (Su), tetracycline (Tc) (ApCmFfSmSpSuTc profile). Recently, variant SGI1 clusters conferring different profiles have been...
ObjectivesClinical isolates of Salmonella enterica serovar Haifa and Newport, which displayed extended multidrug resistance phenotypes, were investigated for the presence genomic island 1 (SGI1) genetic organization its antibiotic gene clusters.
We report the dissemination of a conjugative IncI1 plasmid carrying bla(CTX-M-1), conferring resistance to extended-spectrum cephalosporins, in Salmonella enterica isolates from poultry and humans France 2003 2008. By subtyping, this was shown be genetically related that found Escherichia coli healthy France.
To determine the complete nucleotide sequence of multidrug resistance IncA/C plasmid pR55 from a clinical Klebsiella pneumoniae strain that was isolated urinary tract infection in 1969 French hospital and compare it with those contemporary emerging plasmids. The purified sequenced using 454 sequencing approach. After draft assembly, additional PCRs walking reads were performed for gap closure. Sequence comparisons multiple alignments other plasmids done BLAST algorithm CLUSTAL W,...
Since its initial identification in epidemic multidrug-resistant Salmonella enterica serovar Typhimurium DT104 strains, several SGI1 variants, lineages, and SGI1-related elements (SGI2, PGI1, AGI1) have been described many bacterial genera ( , Proteus Morganella Vibrio Shewanella etc.). They constitute a family of multidrug resistance site-specific integrative acquired by horizontal gene transfer, being the best-characterized element. The transfer SGI1/PGI1 into other is public health...
Abstract During infection, Salmonella senses and responds to harsh environments within the host. Persistence in a bile-rich environment is important for infect small intestine or gallbladder multidrug efflux system AcrAB-TolC required bile resistance. The genes encoding this are mainly regulated by ramRA locus, which composed of divergently transcribed ramA ramR genes. acrAB tolC transcriptionally activated RamA, whose gene itself repressed RamR. RamR recognizes multiple drugs; however,...
To understand the evolutionary dynamics of extended-spectrum β-lactamase (ESBL)-encoding genes in Escherichia coli, we undertook a comparative genomic analysis 116 whole plasmid sequences human or animal origin isolated over period spanning before and after use third-generation cephalosporins (3GCs) using gene-sharing network approach. The plasmids included 82 conjugative, 22 mobilizable 9 non-transferable 3 P-like bacteriophages. ESBL-encoding were found on 64 6 mobilizable, 2 P1-like...
Salmonella enterica bla(CTX-M-2) and bla(CTX-M-9) plasmid backbones from isolates Belgium France were analyzed. The bla(CTX-M-2-)plasmids both human poultry related to the IncHI2 pAPEC-O1-R plasmid, previously identified in United States avian Escherichia coli strains; plasmids closely R478 plasmid.
Salmonella genomic island 1 (SGI1) and variants (SGI1-I the new variant SGI1-O) were mapped in five strains of Proteus mirabilis isolated from humans food China. Sequencing showed that SGI1 integrated at 3' end chromosomal thdF gene as previously described for strains.
This study was designed to determine the genetic basis of florfenicol andceftiofur resistance in Escherichia coli isolates recovered fromFrench cattle. In these isolates, ceftiofur conferred by bla CMY-2 located on three distinct conjugative plasmids ona specific DNA fragment, ISEcp1-bla CMY-2-blc-sugE. Two also carried floR gene conferringresistance florfenicol. The shown be associatedwith insertion sequence ISCR2. Mobile elements appear contributeto mobilization and genesin E. coli....
ABSTRACT For 100 years, it has been obvious that Salmonella enterica strains sharing the serotype with formula 1,4,[5],12:b:1,2—now known as Paratyphi B—can cause diseases ranging from serious systemic infections to self-limiting gastroenteritis. Despite considerable predicted diversity between carrying common B serotype, there remain few methods subdivide group into groups are congruent their disease phenotypes. therefore represents one of canonical examples in where serotyping combined...
To characterize MDR genomic islands related to Salmonella island 1 (SGI1) and Proteus (PGI1) in mirabilis from human animal sources France light of the previously reported cases.A total 52 46 P. clinical strains sources, respectively, were studied for period 2010-13. was assessed by antimicrobial susceptibility testing, PCR detection SGI1 PGI1 mapping regions. The diversity SGI1/PGI1-positive PFGE.Twelve (5 humans 7 dogs) found harbour an or PGI1. Among them, several variants identified...