Olivier Barraud
A5033001823- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Pneumonia and Respiratory Infections
- Antimicrobial Resistance in Staphylococcus
- Nosocomial Infections in ICU
- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Use and Resistance
- Clostridium difficile and Clostridium perfringens research
- Vibrio bacteria research studies
- Enterobacteriaceae and Cronobacter Research
- Listeria monocytogenes in Food Safety
- Bacterial biofilms and quorum sensing
- Escherichia coli research studies
- Diphtheria, Corynebacterium, and Tetanus
- Genomics and Phylogenetic Studies
- Mycobacterium research and diagnosis
- Bacterial Infections and Vaccines
- Respiratory Support and Mechanisms
- Gut microbiota and health
- Sepsis Diagnosis and Treatment
- Bacteriophages and microbial interactions
Université de Limoges
2016-2025
Inserm
2016-2025
Hôpital Universitaire Dupuytren
2012-2024
Hôpital Dupuytren
2011-2024
Centre d’Investigation Clinique 1436
2024
Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques
2020-2024
CNR de la Résistance aux Antibiotiques
2020-2024
Clinical Investigation Center Plurithematic Tours
2024
Centre Hospitalier Universitaire de Limoges
2014-2023
University of Geneva
2023
The spread of antibiotic-resistant bacteria is a growing problem and public health issue. In recent decades, various genetic mechanisms involved in the resistance genes among have been identified. Integrons - elements that acquire, exchange, express embedded within gene cassettes (GC) are one these mechanisms. widely distributed, especially Gram-negative bacteria; they carried by mobile elements, plasmids, transposons, which promote their bacterial communities. Initially studied mainly...
ObjectivesIntegrons are bacterial genetic elements that can capture and express genes contained in mobile cassettes. Integrons have been described worldwide Gram-negative bacteria a marker of antibiotic resistance. We developed specific sensitive Taqman® probe-based real-time PCR method with three different primer–probe pairs for simultaneous detection the main classes integron.
Antibiotic-induced modulation of the intestinal microbiota can lead to Clostridioides difficile infection (CDI), which is associated with considerable morbidity, mortality, and healthcare-costs globally. Therefore, identification markers predictive CDI could substantially contribute guiding therapy decreasing burden. Here, we analyze hospitalized patients at increased risk in a prospective, 90-day cohort-study before after antibiotic treatment diarrhea onset. We show that developing already...
Abstract Background Ventilator-associated pneumonia caused by Pseudomonas aeruginosa (PA) in hospitalised patients is associated with high mortality. The effectiveness of the bivalent, bispecific mAb MEDI3902 (gremubamab) preventing PA nosocomial was assessed PA-colonised mechanically ventilated subjects. Methods EVADE (NCT02696902) a phase 2, randomised, parallel-group, double-blind, placebo-controlled study Europe, Turkey, Israel, and USA. Subjects ≥ 18 years old, ventilated, tracheally...
The ESCPM group (Enterobacter species including Klebsiella aerogenes - formerly Enterobacter aerogenes, Serratia species, Citrobacter freundii complex, Providencia and Morganella morganii) has not yet been incorporated into systematic surveillance programs. We conducted a multicentre retrospective observational study analysing all strains isolated from blood cultures in 27 European hospitals over 3-year period (2020-2022). Diagnostic approach, epidemiology, antimicrobial susceptibility were...
Integrons are bacterial genetic elements able to capture and express genes contained within mobile gene cassettes. Gene cassettes expressed via a Pc promoter can be excised from or integrated into the integron by integrase IntI. Although mechanisms of cassette integration excision well known, kinetics modes shuffling leading new arrays remain puzzling. It has been proposed that under antibiotic selective pressure, IntI-mediated rearrangements generate variants in which weakly moves closer...
Building the European Antimicrobial Resistance Surveillance network in Veterinary medicine (EARS-Vet) was proposed to strengthen One Health antimicrobial resistance (AMR) surveillance approach.
Despite being implicated in a wide spectrum of community- and healthcare-acquired infections, anaerobes have not yet been incorporated into systematic surveillance programs Europe. We conducted multicentre retrospective observational study analysing all anaerobic strains isolated from blood cultures 44 European Hospital Centres over 4-year period (2020-2023). Diagnostic approach, epidemiology, antimicrobial susceptibility according to EUCAST v. 15.0 were investigated. Our included 14,527...
Since its initial identification in epidemic multidrug-resistant Salmonella enterica serovar Typhimurium DT104 strains, several SGI1 variants, lineages, and SGI1-related elements (SGI2, PGI1, AGI1) have been described many bacterial genera ( , Proteus Morganella Vibrio Shewanella etc.). They constitute a family of multidrug resistance site-specific integrative acquired by horizontal gene transfer, being the best-characterized element. The transfer SGI1/PGI1 into other is public health...
Abstract Background Campylobacter spp. bacteremia is a severe infection. A nationwide 5-year retrospective study was conducted to characterize its clinical features and prognostic factors. Methods The included patients with diagnosed in 37 French hospitals participating the surveillance network of National Reference Center for Campylobacters Helicobacters, from 1 January 2015 31 December 2019. goal analyze effects delay appropriate antibiotic therapy other risk factors on 30-day mortality...
In Enterobacterales, the most common carbapenemases are Ambler's class A (KPC-like), B (NDM-, VIM- or IMP-like) D (OXA-48-like) enzymes. This study describes characterization of twenty-four OXA-23 OXA-58 producing-Proteus mirabilis isolates recovered from human and veterinary samples France Belgium. Twenty-two P. producing either (n = 21) 1), collected between 2013 2018, as well 2 reference strains isolated in 1996 2015 were fully sequenced. Phylogenetic analysis revealed that 22 24...
Abstract Trial enrichment using gut microbiota derived biomarkers by high-risk individuals can improve the feasibility of randomized controlled trials for prevention Clostridioides difficile infection (CDI). Here, we report in a prospective observational cohort study incidence CDI and assess potential clinical characteristics to predict 1,007 patients ≥ 50 years receiving newly initiated antibiotic treatment with penicillins plus beta-lactamase inhibitor, 3 rd /4 th generation...
BackgroundThe characteristics and course of endotracheal secretions have scarcely been studied in patients under mechanical ventilation (MV) at risk developing ventilator-associated pneumonia (VAP).Research QuestionCan be exhaustively described what is their predictive value for the diagnosis VAP during MV?Study Design MethodsThis single-center prospective study included neuro-injured with neurologic injury requiring MV least 7 days. Patients pulmonary infectious diseases were ineligible....
Integrons have a well-established role in the dissemination of resistance among Gram-negative pathogens and are thus useful marker antibiotic resistance. Shigellae noteworthy for their multiple drug resistance, having gradually acquired to most widely use inexpensive antimicrobial drugs.A total 32 Shigella strains belonging serotypes flexneri, dysenteriae, boydii 20, new serovar, resistant at least four antibiotics were analyzed by molecular techniques.Class 1 integrons prevalent (92.8%);...