A5019789649- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Urinary Tract Infections Management
- Bacterial Genetics and Biotechnology
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Infections and bacterial resistance
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- Legionella and Acanthamoeba research
- Infective Endocarditis Diagnosis and Management
- Pneumonia and Respiratory Infections
- Cystic Fibrosis Research Advances
- Protist diversity and phylogeny
- Occupational exposure and asthma
- Antimicrobial Resistance in Staphylococcus
- Photoreceptor and optogenetics research
- RNA and protein synthesis mechanisms
- Listeria monocytogenes in Food Safety
- Antifungal resistance and susceptibility
- Antimicrobial Peptides and Activities
- Antibiotics Pharmacokinetics and Efficacy
- Viral gastroenteritis research and epidemiology
- Bladder and Urothelial Cancer Treatments
- Streptococcal Infections and Treatments
Université de Toulouse
2018-2025
Centre Hospitalier Universitaire de Toulouse
2018-2025
Université Toulouse III - Paul Sabatier
2018-2025
Digestive Health Research Institute
2018-2023
École Nationale Vétérinaire de Toulouse
2018-2023
Inserm
2018-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2023
Hôpital Purpan
2019-2023
Institut de Recherche Vaccinale
2017
Laboratoire National de Référence
2017
Although Escherichia coli Nissle 1917 (EcN) has been used therapeutically for over a century, the determinants of its probiotic properties remain elusive. EcN produces two siderophore-microcins (Mcc) responsible an antagonistic activity against other Enterobacteriaceae. also synthesizes genotoxin colibactin encoded by pks island. Colibactin is virulence factor and putative pro-carcinogenic compound. Therefore, we aimed to decouple from genotoxic activity. We demonstrated that pks-encoded...
The ESCPM group (Enterobacter species including Klebsiella aerogenes - formerly Enterobacter aerogenes, Serratia species, Citrobacter freundii complex, Providencia and Morganella morganii) has not yet been incorporated into systematic surveillance programs. We conducted a multicentre retrospective observational study analysing all strains isolated from blood cultures in 27 European hospitals over 3-year period (2020-2022). Diagnostic approach, epidemiology, antimicrobial susceptibility were...
ABSTRACT Achromobacter xylosoxidans is an aerobic nonfermentative Gram-negative rod considered important emerging pathogen among cystic fibrosis (CF) patients worldwide and immunocompromised patients. This increased prevalence remains unexplained, to date no environmental reservoir has been identified. The aim of this study was identify potential reservoirs A. in hospital, domestic, outdoor environments compare the isolates with clinical ones. From 2011 2012, 339 samples were collected...
The pks island codes for the enzymes necessary synthesis of genotoxin colibactin, which contributes to virulence Escherichia coli strains and is suspected promoting colorectal cancer. From a collection 785 human bovine E. isolates, we identified 109 carrying highly conserved island, mostly from phylogroup B2, but also phylogroups A, B1 D. Different scenarios acquisition were deduced whole genome sequence phylogenetic analysis. In main scenario, was introduced stabilized into certain types...
Despite being implicated in a wide spectrum of community- and healthcare-acquired infections, anaerobes have not yet been incorporated into systematic surveillance programs Europe. We conducted multicentre retrospective observational study analysing all anaerobic strains isolated from blood cultures 44 European Hospital Centres over 4-year period (2020-2023). Diagnostic approach, epidemiology, antimicrobial susceptibility according to EUCAST v. 15.0 were investigated. Our included 14,527...
Abstract Background Although less virulent than Pseudomonas aeruginosa, non-aeruginosa (NAP) are opportunistic pathogens that cause invasive infections, mainly in immunosuppressed or intensive care patients. MDR strains of NAP increasingly isolated, especially MBL-producing isolates. Objectives We evaluated the activity cefiderocol, ceftazidime/avibactam and ceftolozane/tazobactam against a collection clinical isolates NAP, which was voluntarily enriched with resistant strains. Methods...
Urinary tract infections (UTIs) are among the most common outpatient infections, with a lifetime incidence of around 60% in women. We analysed urine samples from 223 patients community-acquired UTIs and report presence cleavage product released during synthesis colibactin, bacterial genotoxin, 55 examined. Uropathogenic Escherichia coli strains isolated these patients, as well archetypal E . strain UTI89, were found to produce colibactin. In murine model UTI, machinery producing colibactin...
Objectives: A previous study on 12 in vitro-selected azithromycin-resistant Legionella pneumophila lineages showed that ribosomal mutations were major macrolide resistance determinants. In addition to these mechanisms have been well described many species, upstream of lpeAB operon, homologous acrAB Escherichia coli, identified two lineages. this study, we investigated the role LpeAB and L. pneumophila. Methods: The was studied by testing antibiotic susceptibility WT, deleted complemented...
A functional synergy was previously demonstrated between microcin, salmochelin and colibactin islands in Escherichia coli strains from B2 phylogroup. We aimed to determine this association prevalence uropathogenic E. coli, whether it predictive of the infection severity a collection 225 urinary samples. The high triad, even if wasn't correlated with severity, suggested that might not be virulence factor per se within tract, but would promote its colonization. This triad enable strain...
Abstract Objectives Performance evaluation of routine laboratory methods to determine the susceptibility Enterobacterales urinary isolates fosfomycin (oral administration) and mecillinam. Methods We collected 347 from monomicrobial midstream urine samples women with significant bacteriuria leukocyturia. Mostly non-Escherichia coli (i.e. Klebsiella spp., Citrobacter koseri, Enterobacter cloacae complex Proteus mirabilis) were included (n = 298). VITEK®2, ETEST®, disc diffusion mecillinam was...
Importance Infective endocarditis (IE) caused by Staphylococcus aureus is associated with high mortality, approximately 20% to 30%, mostly in the first month, no improvement recent decades. Current opinion that antistaphylococcal penicillin and cefazolin are equally effective treating methicillin-susceptible S (MSSA) IE, both recommended as possible first-line treatments. Most MSSA strains carry β-lactamase blaZ gene, some -positive exhibit an inoculum effect, meaning increased minimum...
Streptococcus anginosus group (SAG) strains are common pathogens causing abscesses and bacteraemia. They generally susceptible to β-lactams, which constitute first-line treatment. EUCAST recommends testing penicillin G susceptibility screen for β-lactam resistance. Isolates categorized as (negative screening) can be reported aminopenicillins third-generation cephalosporins.To assess the reliability of resistance screening in predicting SAG blood culture isolates, investigate isolates this...
Abstract Urinary tract infections (UTIs) are among the most common outpatient infections, with a lifetime incidence of around 60% in women. We analysed urine samples from 223 patients community-acquired UTIs and report presence metabolite released during synthesis colibactin, bacterial genotoxin, 50 examined. Uropathogenic Escherichia coli strains isolated these patients, as well archetypal E. strain UTI89, were found to produce colibactin. In murine model UTI, machinery producing colibactin...
Background Hazelnut-allergic children express variable clinical phenotypes. Tests for the diagnosis of hazelnut allergy are usually conducted on presenting symptoms following ingestion, but also as screening tests after discovery a food allergy, mainly to tree nut or peanut. It is important determine if molecular allergens could be markers severity in allergy.
ABSTRACT The pks island codes for the enzymes necessary synthesis of genotoxin colibactin, which contributes to virulence Escherichia coli strains and is suspected promoting colorectal cancer. From a collection 785 human bovine E. isolates, we identified 109 carrying highly conserved island, mostly from phylogroup B2, but also phylogroups A, B1 D. Different scenarios acquisition were deduced whole genome sequence phylogenetic analysis. In main scenario, was introduced stabilized into certain...
Urinary tract infections (UTIs) are predominantly caused by uropathogenic Escherichia coli (UPEC). By analysing a representative collection of UPEC strains from community-acquired infections, we showed that 20 % these had the ability to produce protein HlyF. These hlyF + were most virulent, mostly responsible for pyelonephritis, often with bloodstream infections. Using mouse model UTI, HlyF was associated develop urosepsis, presence bacteria in spleen and an exacerbated inflammatory...