Ángel Rodríguez-Villodres
A5026591010- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Use and Resistance
- Pneumonia and Respiratory Infections
- Bacterial Identification and Susceptibility Testing
- Urinary Tract Infections Management
- COVID-19 Clinical Research Studies
- Salmonella and Campylobacter epidemiology
- Clostridium difficile and Clostridium perfringens research
- Listeria monocytogenes in Food Safety
- SARS-CoV-2 and COVID-19 Research
- Vibrio bacteria research studies
- Escherichia coli research studies
- Antimicrobial Resistance in Staphylococcus
- Patient Satisfaction in Healthcare
- Gut microbiota and health
- Infections and bacterial resistance
- Long-Term Effects of COVID-19
- Nosocomial Infections in ICU
- Mycobacterium research and diagnosis
- Essential Oils and Antimicrobial Activity
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Amoebic Infections and Treatments
- Medicinal Plants and Neuroprotection
Universidad de Sevilla
2017-2025
Hospital Universitario Virgen del Rocío
2015-2025
Centro de Investigación Biomédica en Red
2023-2025
Instituto de Biomedicina de Sevilla
2017-2024
Instituto de Salud Carlos III
2023-2024
Health Research Institute of the Balearic Islands
2023
University of Fribourg
2019
Pseudomonas aeruginosa healthcare-associated infections are one of the top antimicrobial resistance threats world-wide. In order to analyze current trends, we performed a Spanish nation-wide high-resolution analysis susceptibility profiles, genomic epidemiology and resistome P. over five-year time lapse.A total 3.180 nonduplicated clinical isolates from two surveys in October 2017 2022 were analyzed. MICs 13 antipseudomonals determined by ISO-EUCAST. Multidrug (MDR)/extensively drug...
Due to the emergence of antimicrobial resistance, new alternative therapies are needed. Silver was used treat bacterial infections since antiquity due its known properties. Here, we aimed evaluate in vitro activity colloidal silver (CS) against multidrug-resistant (MDR) Gram-negative and Gram-positive bacteria. A total 270 strains (Acinetobacter baumannii (n = 45), Pseudomonas aeruginosa 25), Escherichia coli 79), Klebsiella pneumoniae 58)], Staphylococcus aureus 34), epidermidis 14),...
The ESCPM group (Enterobacter species including Klebsiella aerogenes - formerly Enterobacter aerogenes, Serratia species, Citrobacter freundii complex, Providencia and Morganella morganii) has not yet been incorporated into systematic surveillance programs. We conducted a multicentre retrospective observational study analysing all strains isolated from blood cultures in 27 European hospitals over 3-year period (2020-2022). Diagnostic approach, epidemiology, antimicrobial susceptibility were...
Human infections by Bordetella bronchiseptica are increasing in recent years. However, due to the lack of clinical susceptibility/resistance breakpoints, antimicrobial treatment is complex. Business Intelligence (BI) a tool that allows record and analyze large amounts data very short time. The aim this study was cohort patients with B. focusing on how BI can help guide empirical therapy Demographic, clinical, microbiological about were recovered. Then, MIC50/90 several antibiotics...
Background/Objectives: Meropenem (MER) is widely used for the treatment of Pseudomonas aeruginosa infections; however, increasing resistance compromises its efficacy. This study aimed to develop a selective culture medium detection meropenem-resistant P. from respiratory specimens within 24 hours. Methods: The performance was challenged using collection 130 clinical strains (85 MER-susceptible, 14 MER-intermediate, and 21 MER-resistant). Subsequently, validation carried out samples. Results:...
Abstract The aim was to assess the ability of nasopharyngeal SARS-CoV-2 viral load at first patient’s hospital evaluation predict unfavorable outcomes. We conducted a prospective cohort study including 321 adult patients with confirmed COVID-19 through RT-PCR in swabs. Quantitative Synthetic RNA cycle threshold values were used calculate log 10 copies/mL. Disease severity end follow up categorized into mild, moderate, and severe. Primary endpoint composite intensive care unit (ICU) admission...
Abstract Objectives Escherichia coli is characterized by three resistance patterns to β-lactams/β-lactamase inhibitors (BLs/BLIs): (i) ampicillin/sulbactam and susceptibility amoxicillin/clavulanic acid piperacillin/tazobactam (RSS); (ii) acid, (RRS); (iii) ampicillin/sulbactam, (RRR). These are acquired consecutively, indicating a potential risk of developing piperacillin/tazobactam, but the precise mechanism this process not completely understood. Methods Clinical isolates incrementally...
Piperacillin-tazobactam resistance (P/T-R) is increasingly reported among Escherichia coli isolates. Although in vitro experiments have suggested that blaTEM gene plays a key role the P/T-R acquisition, no clinical vivo study has yet confirmed of or other genes. Therefore, we aimed to identify mechanisms underlying by following up patients with E. complicated intra-abdominal infections (cIAI) who experienced P/T treatment failure. Four pairs strains, clonally related from four patients, were...
Abstract Background Antimicrobial stewardship programs (ASPs) are recommended in nursing homes (NHs), although data limited. We aimed to determine the clinical and ecological impact of an ASP for NHs. Methods performed a cluster, randomized, controlled trial before–after study with interrupted time-series analyses 14 NHs 30 consecutive months from July 2018 December 2020 Andalusia, Spain. Seven facilities implemented bundle 5 educational measures (general ASP) 7 added 1-to-1 interviews...
Abstract Objectives To analyse the susceptibility profile to cefepime, carbapenems and new β-lactam/β-lactamase inhibitor combinations in Enterobacter cloacae complex Klebsiella aerogenes isolated from intra-abdominal, urinary, respiratory bloodstream infections SMART (Study for Monitoring Antimicrobial Resistance Trends) surveillance study Spain. Methods The susceptibilities of 759 isolates (473 E. 286 K. aerogenes) collected 11 Spanish hospitals 2016 2022 were analysed following EUCAST...
Piperacillin/tazobactam (TZP) is a β-lactam/β-lactamase inhibitor (BL/BLI) recommended for the empirical treatment of severe infections. The excessive and indiscriminate use TZP has promoted emergence TZP-resistant Escherichia coli isolates. Recently, we demonstrated that may contribute to development
Seven CTX-M-27-producing Shigella sonnei strains were isolated at the University Hospital Virgen del Rocío (Seville, Spain) microbiology service from October to November 2021.To offer extensive information on microbiological and molecular epidemiology results of seven S. isolates compare them with other previously documented associated MSM transmission.S. stool samples patients acute diarrhoea identified through biochemical serological typing. Whole characterization was performed by...
The objective of this study was to evaluate the accuracy MDR Direct Flow Chip Kit for detection antimicrobial resistance (AMR) determinants from bacterial colonies. Ninety-two clinical isolates with known AMR genotypically characterized were used. is a microarray-based assay that included 55 beta-lactams (23), quinolones (13), aminoglycosides (5), macrolides sulfonamides (3), colistin (2), vancomycin chloramphenicol (1), and linezolid (1). correctly detects 52 53 tested. cfr gene (linezolid...
Abstract Piperacillin-tazobactam resistance (P/T-R) is increasingly reported among Escherichia coli isolates. Although in vitro experiments have suggested that blaTEM gene plays a key role the P/T-R acquisition, no clinical vivo study has yet confirmed of or other genes. Therefore, we aimed to identify mechanisms underlying by following up patients with E. intraabdominal infections (IAI) who experienced P/T treatment failure. We found higher copy number isolates, generated three different...
To evaluate the clinical and epidemiological impact of a new molecular surveillance strategy based on qPCR to control an outbreak by Serratia marcescens in Neonatal Intensive Care Unit (NICU).We design specific for detection S. rectal swabs patients admitted NICU. We divided study into two periods: (a) pre-PCR, from declaration introduction, (b) PCR period, introduction until was solved. In all cases, isolates were recovered their clonal relationship analysed PFGE. Control measures...
Escherichia coli is the most frequent Gram-negative bacilli involved in intra-abdominal infections. However, despite high mortality rates associated with biliary tract infections due to E. coli, there no study focusing on this pathogen. In study, we have characterized a group of 15 isolates obtained from 12 patients Demographic and clinical data were recovered. Phylogeny, resistome, virulome analysis through whole genome sequencing biofilm formation investigated. Among isolates, predominant...