A5080125556- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Antibiotic Use and Resistance
- Pneumonia and Respiratory Infections
- Cystic Fibrosis Research Advances
- Antimicrobial Peptides and Activities
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Reproductive tract infections research
- Infections and bacterial resistance
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Pharmaceutical and Antibiotic Environmental Impacts
Health Research Institute of the Balearic Islands
2020-2025
Hospital Universitario Son Espases
2019-2025
Instituto de Salud Carlos III
2024-2025
Centro de Investigación Biomédica en Red
2021-2025
Pseudomonas aeruginosa healthcare-associated infections are one of the top antimicrobial resistance threats world-wide. In order to analyze current trends, we performed a Spanish nation-wide high-resolution analysis susceptibility profiles, genomic epidemiology and resistome P. over five-year time lapse.A total 3.180 nonduplicated clinical isolates from two surveys in October 2017 2022 were analyzed. MICs 13 antipseudomonals determined by ISO-EUCAST. Multidrug (MDR)/extensively drug...
To analyse the dynamics and mechanisms of stepwise resistance development to cefiderocol in Pseudomonas aeruginosa.Cefiderocol evolution was analysed WT PAO1, PAOMS (mutS mutator derivate) three XDR clinical isolates belonging ST111, ST175 ST235 clones. Strains were incubated triplicate experiments for 24 h iron-depleted CAMHB with 0.06-128 mg/L cefiderocol. Tubes from highest antibiotic concentration showing growth reinoculated into fresh medium containing concentrations up 128 7...
ABSTRACT A growing number of novel antipseudomonal β-lactams have been introduced in recent years, but the emergence resistance is still a major concern treatment Pseudomonas aeruginosa infections. Here, we compared mutant prevention concentrations (MPCs) and nature first-step resistant mutants to classical P. . MPCs were determined duplicate experiments for ceftazidime, ceftazidime/avibactam, ceftolozane/tazobactam, imipenem, imipenem/relebactam, meropenem, meropenem/vaborbactam, aztreonam,...
A ceftolozane-tazobactam- and ceftazime-avibactam-resistant Pseudomonas aeruginosa isolate was recovered after treatment (including azithromycin, meropenem, ceftolozane-tazobactam) from a patient that had developed ventilator-associated pneumonia COVID-19 infection. Whole-genome sequencing revealed the strain, belonging to ST274, acquired nonsense mutation leading truncated carbapenem porin OprD (W277X), 7-bp deletion (nt213Δ7) in NfxB (negative regulator of efflux pump MexCD-OprJ), two...
We analysed the dynamics and mechanisms of resistance development to imipenem alone or combined with relebactam in Pseudomonas aeruginosa WT (PAO1) mutator (PAOMS; ΔmutS) strains.PAO1 PAOMS strains were incubated for 24 h Mueller-Hinton Broth 0.125-64 mg/L ± 4 mg/L. Tubes from highest antibiotic concentration showing growth reinoculated fresh medium containing concentrations up 64 7 days. Two colonies per strain, replicate experiment early (Day 1) late 7) cultures characterized by...
Ceftazidime-avibactam is a β-lactam/β-lactamase inhibitor combination restricted for the treatment of multidrug-resistant infections Pseudomonas aeruginosa non-susceptible to ceftazidime and resistant carbapenems. Crucially, it has not been studied if its use could allow design or application new stablished evolution-based strategies that exploit increased susceptibility emerges when resistance acquired (collateral sensitivity, CS). Works in field have focused on study CS model strains, but...
To analyse the dynamics and mechanisms of stepwise resistance development to ceftolozane/tazobactam imipenem/relebactam in XDR Pseudomonas aeruginosa clinical strains.XDR isolates belonging ST111 (main mechanisms: oprD-, dacB-, CARB-2), ST175 (oprD-, ampR-G154R) ST235 OXA-2) high-risk clones were incubated for 24 h Müeller-Hinton Broth with 0.125-64 mg/L ceftolozane + tazobactam 4 or imipenem relebactam mg/L. Tubes from highest antibiotic concentration showing growth reinoculated into fresh...
ABSTRACT We report the emergence of cefiderocol resistance during treatment a ST312 Pseudomonas aeruginosa respiratory infection with ceftazidime/avibactam. whole genome sequencing (WGS) revealed that was caused by large genomic deletion, including PiuDC (iron transport system) and AmpD ( ampC negative regulator), driven integration phage DNA. Thus, our findings alert this type deletion could be an efficient (two mechanisms in one step) specific mechanism might occur nonspecifically upon...
Abstract Objectives We aimed to analyse the interplay between impaired iron uptake and β-lactamases on cefiderocol resistance in Pseudomonas aeruginosa. Methods Thirty-one transferable 16 intrinsic P. aeruginosa AmpC (PDC) variants were cloned expressed wild-type (PAO1) uptake-deficient (PAO ΔpiuC) backgrounds. MICs of antipseudomonal β-lactams determined by reference broth microdilution. Results Relative PAO1, deletion piuC caused a specific 16-fold decrease activity but negligible effects...
The mechanisms underlying an in vivo switch the resistance phenotype of P. aeruginosa after ceftazidime-avibactam treatment was investigated. initial isolate (a blood culture) resistant to meropenem but remained susceptible antipseudomonal cephalosporins and combinations with β-lactamase inhibitors. One week therapy, a subsequent rectal swab) recovered from same patient showed opposite phenotype. Whole-genome sequence analysis revealed single SNP difference between both (ST235) isolates,...
Background/Objectives: Acinetobacter baumannii is a globally emerging pathogen with widespread antimicrobial resistance driven by multiple mechanisms, such as altered expression of efflux pumps like AdeABC, placing it priority for research. Driven the lack new treatments, alternative approaches are being explored to combat its infections, among which efficacy-enhancing adjuvants can be found. This study presents and characterizes MV6, synthetic cyclic peptide that boosts aminoglycoside...
ABSTRACT The mechanisms underlying ceftazidime/avibactam resistance development in four susceptible/resistant pairs of GES-5-producing ST235 Pseudomonas aeruginosa clinical isolates were investigated. In three the cases, was driven by a single mutation leading to GES-27 (P162Q), GES-29 (P162A), or novel GES-60 (N136S), as confirmed through cloning experiments. Moreover, these mutations associated with increased cefiderocol MICs but reduced carbapenem, particularly imipenem/relebactam,...
Neisseria gonorrhoeae exhibits alarming antibiotic resistance trends and poses a significant challenge in therapeutic management. This study aimed to explore the association of penA alleles with penicillin-binding protein (PBP) occupancy patterns reduced outer membrane permeability, impacting susceptibility last-line cephalosporins potential β-lactam candidates. The whole genome sequence, MICs PBP IC50s were determined for 12 β-lactams β-lactamase inhibitors 8 clinical isolates varying...