A5024918735- Antibiotic Resistance in Bacteria
- Pharmacogenetics and Drug Metabolism
- Eicosanoids and Hypertension Pharmacology
- Cystic Fibrosis Research Advances
- Hormonal Regulation and Hypertension
- Antibiotics Pharmacokinetics and Efficacy
- Innovations in Medical Education
- Fungal Infections and Studies
- Bacterial biofilms and quorum sensing
- Antifungal resistance and susceptibility
- Pneumonia and Respiratory Infections
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Biosensors and Analytical Detection
- Phytochemical compounds biological activities
- Fungal Biology and Applications
- Inflammatory mediators and NSAID effects
- Streptococcal Infections and Treatments
- Orthopedic Infections and Treatments
- Hemoglobinopathies and Related Disorders
- Simulation-Based Education in Healthcare
- Drug Transport and Resistance Mechanisms
- Advanced biosensing and bioanalysis techniques
- Pharmacy and Medical Practices
- Phytochemistry and Bioactivity Studies
Monash University
2018-2024
Singapore-MIT Alliance for Research and Technology
2023
Universiti Teknologi MARA
2007-2014
Universiti Sains Malaysia
2004-2007
Pseudomonas aeruginosa remains a challenge in chronic respiratory infections cystic fibrosis (CF). Ceftolozane-tazobactam has not yet been evaluated against multidrug-resistant hypermutable P. isolates the hollow-fiber infection model (HFIM). Isolates CW41, CW35, and CW44 (ceftolozane-tazobactam MICs of 4, 2 mg/L, respectively) from adults with CF were exposed to simulated representative epithelial lining fluid pharmacokinetics ceftolozane-tazobactam HFIM. Regimens continuous infusion (CI;...
ObjectivesMeropenem is commonly used against Pseudomonas aeruginosa. Traditionally, the time unbound antibiotic concentration exceeds MIC (fT>MIC) to select carbapenem regimens. We aimed characterize effects of different baseline resistance mechanisms on bacterial killing and emergence; evaluate whether fT>MIC can predict these effects; and, develop a novel Quantitative Systems Pharmacology (QSP) model describe time-course response.MethodsSeven isogenic P. aeruginosa strains with range MICs...
CYP2C8 is genetically polymorphic. Four variants, CYP2C8*2, CYP2C8*3, CYP2C8*4 and CYP2C8*5, which contain mutations in the coding regions have been reported to exhibit different enzyme activity as compared with CYP2C8*1.To determine allele frequency of three codon-changing variants (CYP2C8*2, CYP2C8*3 CYP2C8*4) Malaysian population.Healthy unrelated volunteers from major races Malaysia were recruited. The study was approved by local Research Ethics Committee. DNA extracted using a standard...
P-glycoprotein (PgP) is the most extensively studied ATP-binding cassette (ABC) coded by MDR1 gene. To date, 29 single nucleotide polymorphisms (SNPs) have been identified; but only SNP C3435T has correlated with intestinal PgP expression levels and shown to influence absorption of orally taken drugs that are substrates. Individuals homozygous for T allele more than fourfold lower compared C/C individuals. We developed a one step primer based specific PCR method detect at investigate...
1. A retrospective study was conducted to explore the importance of CYP2C9 genotyping for initiation and maintenance therapy warfarin in clinical practice. total 191 patients on a local hospital were recruited after written informed consent. Their medical records reviewed no intervention dose performed. 2. 5 ml blood taken from each subject DNA extraction identification 1, 2, 3 4 alleles, using nested-allele-specific-multiplex-polymerase chain reaction (PCR). Half Malays remaining Chinese....
Meropenem is commonly used against Pseudomonas aeruginosa ; however, resistance emerging. Traditionally, the time unbound antibiotic concentration exceeds MIC ( f T >MIC ) to select carbapenem regimens. We aimed 1) characterize effects of different mechanisms on bacterial killing and emergence, 2) evaluate whether can predict these effects, 3) develop a novel quantitative systems pharmacology model elucidate baseline time-course response. Seven isogenic P. strains with range MICs were in...
Hypermutable Pseudomonas aeruginosa strains are highly prevalent in chronic lung infections of patients with cystic fibrosis (CF). Acute exacerbations these have limited treatment options. This study aimed to investigate inhaled aztreonam and tobramycin against clinical hypermutable P. using the CDC dynamic vitro biofilm reactor (CBR), mechanism-based mathematical modeling (MBM) genomic studies. Two CF multidrug-resistant were investigated a 168h CBR (n=2 biological replicates). Regimens (75...
Acute rheumatic fever (ARF), an autoimmune reaction to Group A Streptococcus (Streptococcus pyogenes; Strep A) infection, can cause heart disease (RHD). New formulations of long-acting penicillins are being developed for secondary prophylaxis ARF and RHD.
Arthroplasty is a healthcare priority and represents high volume, cost surgery. Periprosthetic joint infection (PJI) results in significant mortality, thus it vital that the risk for PJI minimized. Vancomycin recommended surgical prophylaxis total arthroplasty (TJA) by current clinical practice guidelines endorsed Infectious Diseases Society of America. This study aimed to develop new assay determine vancomycin concentrations serum bone, minimal physiologically based population PK (mPBPK)...