A5070756220- Mycotoxins in Agriculture and Food
- Carcinogens and Genotoxicity Assessment
- Escherichia coli research studies
- Gut microbiota and health
- Wheat and Barley Genetics and Pathology
- Tuberculosis Research and Epidemiology
- Antibiotic Resistance in Bacteria
- Probiotics and Fermented Foods
- Mycobacterium research and diagnosis
- Infant Nutrition and Health
- Dye analysis and toxicity
- Agricultural safety and regulations
- Toxin Mechanisms and Immunotoxins
- Plant and fungal interactions
- Urinary Tract Infections Management
- Potato Plant Research
- Listeria monocytogenes in Food Safety
- Microbial infections and disease research
- Neuroscience and Neural Engineering
- Botanical Research and Chemistry
- Effects of Radiation Exposure
- Nutritional Studies and Diet
- Indoor Air Quality and Microbial Exposure
- Microbial Natural Products and Biosynthesis
- DNA Repair Mechanisms
Inserm
2013-2025
Université de Toulouse
2013-2025
Digestive Health Research Institute
2021-2025
Université Toulouse III - Paul Sabatier
2013-2025
École Nationale Vétérinaire de Toulouse
2017-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2025
Toxalim Research Centre in Food Toxicology
2014-2024
Institut de Pharmacologie et de Biologie Structurale
2017-2023
Centre National de la Recherche Scientifique
2013-2017
Institut National de la Recherche Agronomique
2014-2016
In Escherichia coli, the biosynthetic pathways of several small iron-scavenging molecules known as siderophores (enterobactin, salmochelins and yersiniabactin) a genotoxin (colibactin) are to require 4′-phosphopantetheinyl transferase (PPTase). Only two PPTases have been clearly identified: EntD ClbA. The gene coding for is part core genome E. whereas ClbA encoded on pks pathogenicity island which codes colibactin. Interestingly, physically associated with high (HPI) in subset highly...
The neonatal gut is rapidly colonized by a newly dominant group of commensal Escherichia coli strains among which large proportion produces genotoxin called colibactin. In order to analyze the short- and long-term effects resulting from such evolution, we developed rat model mimicking natural transmission E. mothers neonates. Genotoxic non-genotoxic were equally transmitted offspring stably across generations. DNA damage was only detected in neonates with genotoxic strains. Signs stress as...
Sepsis is a life-threatening infection. Escherichia coli the first known cause of bacteremia leading to sepsis. Lymphopenia was shown predict better than conventional markers The pks genomic island, which harbored by extraintestinal pathogenic E. (ExPEC) and encodes genotoxin colibactin, epidemiologically associated with bacteremia. To investigate possible relationship between colibactin lymphopenia, we examined effects transient infection lymphocytes bacteria that were those not producing...
ABSTRACT An increasing number of human beings from developed countries are colonized by Escherichia coli strains producing colibactin, a genotoxin suspected to be associated with the development colorectal cancers. Deoxynivalenol (DON) is most prevalent mycotoxin that contaminates staple food—especially cereal products—in Europe and North America. This study investigates effect food contaminant DON on genotoxicity E. colibactin. In vitro , intestinal epithelial cells were coexposed vivo...
Deoxynivalenol (DON), one of the most widespread mycotoxins in Europe, and cadmium (Cd), a environmental pollutant, are common food contaminants. They exert adverse effects on different organs including kidney, liver, intestine. The intestine is target DON Cd when they ingested. Most studies have focused their individual whereas combined toxicity has rarely been studied. aim this study was thus to evaluate intestinal barrier function vitro vivo. In vitro, Caco-2 cells were treated with...
Abstract CprA is a short‐chain dehydrogenase/reductase (SDR) that contributes to resistance against colistin and antimicrobial peptides. The cprA gene conserved across Pseudomonas aeruginosa clades its expression directly regulated by the two‐component system PmrAB. We have shown leads production of outer membrane vesicles (OMVs) block autophagic flux greater capacity activate non‐canonical inflammasome pathway. In murine model sepsis, P. strain deleted for was less virulent than wild‐type...
ABSTRACT The intestinal barrier controls the balance between tolerance and immunity to luminal antigens. When this finely tuned equilibrium is deregulated, inflammatory disorders can occur. There a concomitant increase, in urban populations of developed countries, immune-mediated diseases along with shift Escherichia coli population from declining phylogenetic group A newly dominant B2, including commensal strains producing genotoxin called colibactin that massively colonized gut neonates....
Protein O-mannosylation is crucial for the biology of Mycobacterium tuberculosis but key mannosylated protein(s) involved and its(their) underlying function(s) remain unknown. Here, we demonstrated that M. mutant (Δpmt) deficient protein exhibits enhanced release lipoarabinomannan (LAM) in a complex with LprG, lipoprotein required LAM translocation to cell surface. We determined LprG O-mannosylated at unique threonine position by mass spectrometry analyses purified protein. However, although...
Introduction The food contamination by mycotoxins is of increasing public health concerns. Deoxynivalenol (DON), a mycotoxin contaminating cereals, has been associated with the exacerbation inflammatory bowel diseases (IBD), thereby raising question its role in development IBD. Moreover, effect DON on colon poorly described. Methods and Results Wistar rats exposed (1–4 weeks) to low doses (2 or 9 mg kg −1 feed) show microscopic alterations colonic tissue (dilated lymphatic vessels, luminal...
The molecular factors and genetic adaptations that contributed to the emergence of Mycobacterium tuberculosis (MTB) from an environmental canettii-like ancestor, remain poorly investigated. In MTB, PhoPR two-component regulatory system controls production secretion proteins lipid virulence effectors. Here, we describe several mutations, present in phoR M. canettii relative impact expression PhoP regulon pathogenicity strains. First, establish a model PhoR show some substitutions found are...
Deoxynivalenol (DON) is one of the most prevalent type B trichothecenes present in food inducing adverse effects, including intestinal changes and immunosuppression. The aim study was to investigate effects DON on rats exposed for 7, 14 28 days mycotoxin-contaminated diets, using histological immunohistochemical analyses liver lymphoid organs. Fifty received a control diet, or diet contaminated with 1.75 mg/kg 30 days, 11.4 days. Ingestion feed induced significant increase lesional score...
Probiotics represents an alternative to replace antibiotics as growth promoters in animal feed and are able control enteric bacterial diseases improve gut immunity. Saccharomyces cerevisiae RC016 showed previously inhibition/coagregation of pathogens) mycotoxins adsorbent ability (aflatoxin B 1 , ochratoxin A zearalenone). The aim this work was evaluate beneficial properties S. a non-inflammatory vivo model weaned piglets intestinal inflammation ex induced by the mycotoxin deoxynivalenol...
Mycobacterium tuberculosis , the main causative agent of human tuberculosis, is transmitted from person to via small droplets containing very few bacteria. Optimizing chance seed in lungs therefore a major adaptation favor survival and dissemination population. Here we used TnSeq identify genes important for early events leading bacterial seeding lungs. Beside several encoding known virulence factors, found three new candidates not previously described: rv0180c rv1779c rv1592c . We focused...