A5081021140- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Ovarian function and disorders
- Sexual Differentiation and Disorders
- Reproductive Biology and Fertility
- Estrogen and related hormone effects
- Hormonal and reproductive studies
- CAR-T cell therapy research
- Immune Response and Inflammation
- Myasthenia Gravis and Thymoma
- Cytokine Signaling Pathways and Interactions
- Cervical Cancer and HPV Research
- Atherosclerosis and Cardiovascular Diseases
- Immunodeficiency and Autoimmune Disorders
- Reproductive System and Pregnancy
- Reproductive Health and Contraception
- Virus-based gene therapy research
- Endometriosis Research and Treatment
- Systemic Lupus Erythematosus Research
- Multiple Sclerosis Research Studies
- Urological Disorders and Treatments
- Cancer Research and Treatments
- Escherichia coli research studies
- Inflammasome and immune disorders
Université de Toulouse
2011-2024
Université Toulouse III - Paul Sabatier
2009-2024
Inserm
2008-2024
Centre National de la Recherche Scientifique
2002-2024
University of Alberta
2019-2024
Centre de Recherche en Cancérologie de Toulouse
2024
Centre de Physiopathologie de Toulouse-Purpan
2006-2018
Institut National de Recherche en Santé Publique
2013
Centre National pour la Recherche Scientifique et Technique (CNRST)
2011
Agricultural Research Service
2011
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) with an increasing incidence in developed countries. Recent reports suggest that modulation gut microbiota might be one promising therapy for MS. Here, we investigated whether probiotic Escherichia coli strain Nissle 1917 (ECN) could modulate outcome experimental autoimmune encephalomyelitis (EAE), murine model We evidenced daily oral treatment ECN, but not archetypal K12 E. MG1655, reduced severity...
Autoreactive anti–MHC class II T cells are found in Brown Norway (BN) and Lewis (LEW) rats that receive either HgCl2 or gold salts. These have a helper cell 2 (Th2) phenotype the former strain responsible for Th2-mediated autoimmunity. In contrast, expand LEW produce IL-2 prevent experimental autoimmune encephalomyelitis, cell-mediated disease. The aim of this work was to investigate, using lines derived from HgCl2-injected (LEWHg), effect these autoreactive on development five LEWHg...
Abstract Experimental autoimmune myasthenia gravis (EAMG) is a T cell-dependent, Ab-mediated disease induced in rats by single immunization with acetylcholine receptor (AChR). Although polarized Th1 responses have been shown to be crucial for the development of mouse EAMG, role Th cell subsets rat EAMG not well established. In present work we show that while incidence and severity are similar Lewis (LEW) Brown-Norway (BN) rats, strong differences revealed immune response generated....
VAV1 plays a role in regulating proinflammatory cytokines, which underlie the susceptibility for developing experimental autoimmune encephalomyelitis and multiple sclerosis.
VAV1 mediates cross-talk between the CD226 and TCR signaling pathways to enhance production of proinflammatory cytokine IL-17.
Abstract Graft‐versus‐host disease (GvHD) is a major complication of allogeneic bone marrow transplantation and occurs when donor T cells react with histo‐incompatible recipient's antigens. In thepresent study, we analyzed the contribution CD4 cell subsets, defined according to their CD45RC expression level, in development acute chronic GvHD. For this purpose, used model GvHD induced rats parental lymphocytes are transferred irradiated (LEW×BN) F1 hybrid recipients. We showed that high...
Regulatory T cells have been described to specifically accumulate at the site of regulation together with effector and antigen-presenting cells, establishing a state local immune privilege. However mechanisms this interplay remain be defined. We previously demonstrated, in fully MHC mismatched rat cardiac allograft combination, that short-term treatment deoxyspergualine analogue, LF15-0195, induces long-term tolerance specific expansion regulatory CD4+CD25+T within graft. In study, we show...
Spontaneous or chemically induced germline mutations, which lead to Mendelian phenotypes, are powerful tools discover new genes and their functions. Here, we report an autosomal recessive mutation that occurred spontaneously in a Brown-Norway (BN) rat colony was identified as causing marked T cell lymphopenia. This stabilized strain, named BN(m) for "BN mutated." In rats, found the lymphopenia originated thymus, intrinsic CD4 lymphocytes, associated with development of inflammatory bowel...
ABSTRACT The intestinal barrier controls the balance between tolerance and immunity to luminal antigens. When this finely tuned equilibrium is deregulated, inflammatory disorders can occur. There a concomitant increase, in urban populations of developed countries, immune-mediated diseases along with shift Escherichia coli population from declining phylogenetic group A newly dominant B2, including commensal strains producing genotoxin called colibactin that massively colonized gut neonates....
In animal models of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV), the proportion CD45RC T cell subsets is important for disease susceptibility. Their human counterparts are, however, functionally ill defined. this report, we studied their distribution in healthy controls (HC), AAV patients and Systemic lupus erythematous (SLE) as controls. We showed that expression level on CD4 CD8 cells identifies are highly variable among individuals. Interestingly, exhibit an...
Abstract Objective To identify novel genetic loci that predispose to early‐onset myasthenia gravis ( EOMG ) applying a two‐stage association study, exploration, and replication strategy. Methods Thirty‐four one confirmation loci, human leukocyte antigen HLA )‐ DRA , were selected as candidate genes by team members of groups involved in different research aspects MG . In the exploration step, these genotyped 384 matched controls significant difference allele frequency found eight genes. 1177...
Abstract Differential cytokine production by T cells plays an important role in the outcome of immune response. We show that level CD45RC expression differentiates rat CD8 two subpopulations, CD45RChigh and CD45RClow, have different profiles functions. Upon vitro stimulation, Ag-presenting cell-independent system, produce IL-2 IFN-γ while CD45RClow IL-4, IL-10, IL-13. In vitro, these subsets also exhibit cytotoxic suppressive The CD45RChigh/CD45RClow cell ratio was determined Lewis (LEW)...
Abstract During their development, immature CD4CD8 double positive thymocytes become committed to either the CD4 or CD8 lineage. The final size of peripheral and T cell compartments depends on thymic output differential survival proliferation respective subsets in periphery. Our results reveal that development distinct CD4/CD8 ratio between Lewis Brown Norway rats originates thymus and, as shown by use radiation bone marrow chimeras, is determined selection radio-resistant stromal cells....
Abstract We report on a soluble (s) form of CD21 (the C3dg/Epstein‐Barr virus receptor, CR2) that is spontaneously released by B and T lymphocytes. Immunoprecipitation with anti‐CD21 mAb culture supernatants surface biosynthetically labeled cell lines revealed single band an apparent molecular mass 135 kDa. The molecule exhibited 10 kDa lower than membrane CD21. release (sCD21) was time dependent correlated parallel decrease in the expression membrane‐associated molecule. protein also found...
CD4+ regulatory T cells (Treg cells) expressing the transcription factor Foxp3 play a pivotal role in maintaining peripheral tolerance by inhibiting expansion and function of pathogenic conventional (Tconv cells). In this study, we show that locus on rat chromosome 9 controls size natural Treg cell compartment. Fine mapping with interval-specific congenic lines association experiments using single nucleotide polymorphisms (SNPs) identified nonsynonymous SNP Vav1 gene leads to substitution an...
Although transplantation is the common treatment for end-stage renal failure, allograft rejection and marked morbidity from use of immunosuppressive drugs remain important limitations. A major challenge in field to identify easy, reliable noninvasive biomarkers allowing prediction deleterious alloreactive immune responses tailoring therapy individuals according risk. In this study, we first established that expression RC isoform CD45 molecule (CD45RC) on CD4 CD8 T cells healthy identifies...
Regulatory T cells (Treg) represent a minor subpopulation of lymphocytes that is crucial for the maintenance immune homeostasis. Here, we present large-scale quantitative mass spectrometry study defines specific proteomic "signature" Treg. Treg and conventional lymphocyte (Tconv) subpopulations were sorted by flow cytometry subjected to global analysis single-run nanoLC-MS/MS on fast-sequencing Q-Exactive spectrometer. Besides "historical" proteins characterize Treg, our identified numerous...
Experimental allergic encephalomyelitis (EAE) is a T cell-mediated autoimmune disease induced in susceptible rat strains by single immunization with myelin basic protein (MBP). The Lewis (LEW) strain to induction while the Brown Norway (BN) resistant. This resistance involves non-MHC genes since congenic BN-1L rats, LEW MHC on BN-derived background, are also In present study we show that, upon MBP, non-MHC-encoded develop clinical EAE rats associated decreased production of IFN-γ. may be due...