A5077593436- Advanced biosensing and bioanalysis techniques
- Nanowire Synthesis and Applications
- Receptor Mechanisms and Signaling
- Molecular Junctions and Nanostructures
- Lipid Membrane Structure and Behavior
- Luminescence and Fluorescent Materials
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Photoreceptor and optogenetics research
- Neuropeptides and Animal Physiology
- Molecular Sensors and Ion Detection
- Neuroscience and Neural Engineering
- RNA Interference and Gene Delivery
- Quantum Dots Synthesis And Properties
- Ion channel regulation and function
- DNA and Nucleic Acid Chemistry
- Retinal Development and Disorders
- Cellular transport and secretion
- Protein Structure and Dynamics
- Photochromic and Fluorescence Chemistry
- Nicotinic Acetylcholine Receptors Study
- RNA and protein synthesis mechanisms
- Computational Drug Discovery Methods
University of Copenhagen
2015-2024
Indiana University Bloomington
2021
World Health Organization - Denmark
2018
Lundbeck Foundation
2017
Centre National de la Recherche Scientifique
2002-2011
Institut de Biologie Physico-Chimique
2011
University of Florida
2005-2009
Interface (United States)
2005-2009
Xiamen University
2007
Columbia University
2007
Molecular beacon DNA probes, containing 1-4 pyrene monomers on the 5' end and quencher DABCYL 3' end, were engineered employed for real-time probing of sequences. In absence a target sequence, multiple-pyrene labeled molecular beacons (MBs) assumed stem-closed conformation resulting in quenching excimer fluorescence. presence target, switched to stem-open conformation, which separated label from molecule generated an emission signal proportional concentration. Steady-state fluorescence...
Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; structure, dynamics, properties of resulting complexes. All MPs tested date form water-soluble complexes APols, biochemical stability is in general greatly improved compared detergent solutions. The functionality ligand-binding APol-trapped reviewed,...
The lateral organization of a prototypical G protein-coupled receptor, the neurokinin-1 receptor (NK1R), was investigated in living cells by fluorescence resonance energy transfer (FRET) microscopy, taking advantage recently developed acyl carrier protein (ACP) labeling technique. NK1R expressed as fusion with ACP to which small fluorophores were then covalently bound. Our approach allowed recording FRET images receptors on unprecedented high signal-to-noise ratios and subsequent unequivocal...
The perspectives offered by vertical arrays of nanowires for biosensing applications in living cells depend on the access individual to cell interior. Recent results electrical and molecular delivery suggest that direct is not always obtained. Here, we present a generic approach directly visualize membrane conformation interfaced with nanowire arrays, single resolution. method combines confocal z-stack imaging an optimized labelling strategy which was applied HEK293 2–11 μm long 3–7 spaced...
Abstract Nanowires (NWs) are attracting more and interest due to their potential cellular applications, such as delivery of compounds or sensing platforms. Arrays vertical indium‐arsenide (InAs) NWs interfaced with human embryonic kidney cells rat dorsal root ganglion neurons. A selection critical cell functions pathways shown not be impaired, including adhesion, membrane integrity, intracellular enzyme activity, DNA uptake, cytosolic protein expression, the neuronal maturation pathway. The...
Arrays of nanowires (NWs) are currently being established as vehicles for molecule delivery and electrical- fluorescence-based platforms in the development biosensors. It is conceivable that NW-based biosensors can be optimized through increased understanding how nanotopography influences interfaced biological material. Using state-of-the-art homogenous NW arrays allow a systematic investigation broad range densities used by community cells. Here it demonstrated indium arsenide provide...
A method to fabricate inexpensive and transparent nanowire impalement devices is invented based on CuO arrays grown by thermal oxidation. By employing a novel process the nanowires are transferred transparent, cell-compatible epoxy membrane. Cargo delivery detailed cell-nanowire interaction studies performed, revealing that cell plasma membrane tightly wraps nanowires, while penetration not observed. The presented device offers an efficient investigation platform for further optimization,...
Introduction of an isopropyl bridge in the triangulenium skeleton leads to a new series redshifted dyes with high fluorescence quantum yields and remarkable long lifetime allowing for time-gated cell imaging.
Vertical arrays of nanostructures (NSs) are emerging as promising platforms for probing and manipulating live mammalian cells. The broad range applications requires different types interfaces, but cell settling on NS is not yet fully controlled understood. Cells both seen to deform completely into stay suspended like tiny fakirs, which have hitherto been explained with differences in spacing or density. Here, a better understanding this phenomenon provided by using model that takes account...
Abstract Ultrabright fluorescent nanoparticles (NPs) hold great promise for demanding bioimaging applications. Recently, extremely bright molecular crystals of cationic fluorophores were obtained by hierarchical coassembly with cyanostar anion‐receptor complexes. These small‐molecule ionic isolation lattices (SMILES) ensure spatial and electronic to prohibit aggregation quenching dyes. We report a simple, one‐step supramolecular approach formulate SMILES materials into NPs. Rhodamine‐based...
Abstract Experimental autoimmune myasthenia gravis (EAMG) is a T cell-dependent, Ab-mediated disease induced in rats by single immunization with acetylcholine receptor (AChR). Although polarized Th1 responses have been shown to be crucial for the development of mouse EAMG, role Th cell subsets rat EAMG not well established. In present work we show that while incidence and severity are similar Lewis (LEW) Brown-Norway (BN) rats, strong differences revealed immune response generated....
Silicon nanowire (Si NW)-based field effect transistors (FETs) have shown great potential as biosensors (bioFETs) for ultra-sensitive and label-free detection of biomolecular interactions. Their sensitivity depends not only on the device properties, but also function biological recognition motif attached to Si NWs. In this study, we show that SiNWs can be chemically functionalized with Ni:NTA motifs, suitable specific immobilization proteins via a short polyhistidine tag (His-tag) at close...
DNA sensors and microarrays permit fast, simple, real-time detection of nucleic acids through the design use increasingly sensitive, selective, robust molecular probes. Specifically, beacons (MBs) have been employed for this purpose; however, their potential in development solid-surface-based biosensors has not fully realized. This is mainly a consequence beacon's poor stability because hairpin structure once immobilized onto solid surface, commonly resulting low signal enhancement. Here, we...
Because of the importance their physiological functions, cell membranes represent critical targets in biological research. Membrane proteins, which make up approximately 1/3 proteome, interact with a wide range small ligands and macromolecular partners as well foreign molecules such synthetic drugs, antibodies, toxins, or surface recognition proteins pathogenic organisms. Whether it is for sake basic biomedical pharmacological research, great interest to develop tools facilitating study...
Nanowire-based field-effect transistors (FETs) can be used as ultra-sensitive and label-free biosensors for detecting protein–protein interactions. A way to increase the performance of such sensors is dilute sensing buffer drastically. However, we show here that this have an important effect on function proteins. Moreover, it demonstrated dilution significantly affects pH stability buffer, which consequently impacts charge protein thus response signal-to-noise ratio in experiments. Three...
Catecholamine-triggered β-adrenoceptor (β-AR) signaling is essential for the correct functioning of heart. Although both β1 - and β2 -AR subtypes are expressed in cardiomyocytes, drugs selectively targeting have proven this receptor as main target therapeutic effects beta blockers Here, we report a new strategy light-control activation by means photoswitchable with high level -/β2 selectivity. All reported molecules allow an efficient real-time optical control function vitro. Moreover, using...
Over the past decade, vertical nanostructures have provided novel approaches for biomedical applications such as intracellular delivery/detection, specific cell capture, membrane potential measurement, and cellular activity regulation. Although feasibility of a new biological tool has been thoroughly demonstrated, better understanding behavior on nanostructures, in particular effects geometry, is essential advanced applications. To investigate according to variation spacing between we...
Protein microarrays are valuable tools for protein assays. Reducing spot sizes from micro- to nano-scale facilitates miniaturization of platforms and consequently decreased material consumption, but faces inherent challenges in the reduction fluorescent signals compatibility with complex solutions. Here we show that vertical arrays nanowires (NWs) can overcome several bottlenecks using nanoarrays extraction analysis proteins. The high aspect ratio NWs results a large surface area available...
The binding of a fluorescent agonist to the acetycholine receptor from Torpedo electric organ has been studied by time‐resolved spectroscopy in three different environments: native membrane fragments, detergent CHAPS, and after complexation amphipathic polymers (‘amphipols’). Binding kinetics was similar amphipols, demonstrating that can display unaltered allosteric transitions outside its natural lipid environment. In contrast, equilibria were strongly shifted towards desensitized state...
Nanometer light source: Interaction between high-affinity binding sites on lipid-coated nanocrystals (NCs) and different proteins enables the controlled formation of supramolecular nanostructures micropatterned surfaces (see picture; FRET=fluorescence resonant energy transfer, NTA=nitrilotriacetic acid, His6=hexahistidine). The NCs serve as local sources emitting over distances from 1 to more than 10 nm.
Abstract Cell‐membrane sheets suitable for in‐vitro functional fluorescence studies have been prepared by direct detachment from cell membranes using poly‐ L ‐lysine‐coated glass slides. The resulting transferred planar conserve the composition as well most properties of original plasma membrane; in particular, both membrane leaflets remain fluid, allowing investigation diffusion different cellular components. Measurements on offer several advantages compared to those living cells. First,...
The ability to detect changes in gene expression, especially real-time and with sensitivity sufficient enough monitor small variations a single-cell, will have considerable value biomedical research applications. Out of the many available molecular probes for intracellular monitoring nucleic acids, beacon (MB) is most frequently used probe advantages high selectivity. However, any processes which MB stem-loop structure broken result restoration fluorescence MB. This brings few possibilities...