A5017677641- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- RNA and protein synthesis mechanisms
- Photoreceptor and optogenetics research
- Regulation of Appetite and Obesity
- NMR spectroscopy and applications
- Polysaccharides and Plant Cell Walls
- Advanced NMR Techniques and Applications
- Force Microscopy Techniques and Applications
- Biochemical Analysis and Sensing Techniques
- Fuel Cells and Related Materials
- Microbial Metabolites in Food Biotechnology
- Neuroscience and Neuropharmacology Research
- Mass Spectrometry Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- Molecular spectroscopy and chirality
- Molecular Sensors and Ion Detection
- Catalysis and Oxidation Reactions
- Polysaccharides Composition and Applications
- Monoclonal and Polyclonal Antibodies Research
- Thermochemical Biomass Conversion Processes
Institut de Biologie Physico-Chimique
2010-2025
Laboratoire de Biologie Physico-Chimique des Protéines Membranaires
2009-2023
Centre National de la Recherche Scientifique
1998-2021
Université Paris Cité
2012-2021
Sorbonne Université
2005-2019
Sorbonne Paris Cité
2016-2019
Université Paris Sciences et Lettres
2017-2018
Délégation Paris 7
2017-2018
Laboratoire de Génie des Procédés Catalytiques
2016
Laboratoire de Chimie
1992-2015
Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; structure, dynamics, properties of resulting complexes. All MPs tested date form water-soluble complexes APols, biochemical stability is in general greatly improved compared detergent solutions. The functionality ligand-binding APol-trapped reviewed,...
Well characterized pectin samples were incubated with cell wall-bound and -solubilized pure isoforms of pectinmethylesterase from mung bean hypocotyls (Vigna radiata). Both enzyme activity average product structure determined at intervals along the deesterification pathway pH 5.6 7.6. The latter analyses performed by 13C NMR spectroscopy, degree esterification was probed both potentiometric measurements. A dichotomy observed in behavior alpha gamma when compared that beta isoenzyme. Ideal...
Mapping the conformational landscape of G protein-coupled receptors (GPCRs), and in particular how this is modulated by membrane environment, required to gain a clear picture signaling proceeds. To end, we have developed an original strategy based on solution-state nuclear magnetic resonance combined with efficient isotope labeling scheme. This was applied typical GPCR, leukotriene B4 receptor BLT2, reconstituted lipid bilayer. Because this, are able provide direct evidence that BLT2...
Nonionic amphipols (NAPols) synthesized by homotelomerization of an amphiphatic monomer are able to keep membrane proteins (MPs) stable and functional in the absence detergent. Some their biochemical biophysical properties applications have been examined, with particular attention being paid complementarity classical polyacrylate-based amphipol A8-35. Bacteriorhodopsin (BR) from Halobacterium salinarum cytochrome b(6)f complex Chlamydomonas reinhardtii were found be native state highly...
One of the major obstacles to membrane protein (MP) structural studies is destabilizing effect detergents. Amphipols (APols) are short amphipathic polymers that can substitute for detergents keep MPs water-soluble under mild conditions. In present work, we have explored feasibility studying structure APol-complexed by NMR. As a test MP, chose 171-residue transmembrane domain outer MP A from Escherichia coli (tOmpA), whose x-ray and NMR structures in detergent known. 2 H, 15 N-labeled tOmpA...
A novel type of nonionic amphipols for handling membrane proteins in detergent-free aqueous solutions has been obtained through free-radical homo-telomerization an acrylamide-based monomer comprising a C(11) alkyl chain and two glucose moieties, using thiol as transfer reagent. By controlling the thiol/monomer ratio, number-average molecular weight polymers was varied from 8 to 63 kDa. Homopolymeric were found be highly soluble water self-organize, within large concentration range, into...
Abstract The membrane is an integral component of the G protein-coupled receptor signaling machinery. Here we demonstrate that lipids regulate efficacy and selectivity ghrelin GHSR through specific interactions bulk effects. We find PIP2 shifts conformational equilibrium away from its inactive state, favoring basal agonist-induced protein activation. This occurs because a preferential binding to intracellular sites in active state. Another lipid, GM3, also binds favors activation, but mostly...
Biological membranes are complex environments whose functions closely tied to the dynamic interactions between lipids and proteins. Here, we utilize high-pressure NMR of lipid nanodiscs paired with molecular dynamics simulations elucidate at atomic scale allosteric dialog bilayer a bacterial model membrane protein, OmpX. We discover that OmpX delays gelation process by liquefying annular shell through hydrophobic roughness matching processes protein surface. Furthermore, alteration...
G protein-coupled receptors (GPCRs) are key players in signal recognition and cell communication among the most important targets for drug development. Direct structural information on conformation of GPCR ligands bound to their is scarce. Using a leukotriene receptor, BLT2, expressed under perdeuterated form Escherichia coli , purified milligram amounts, folded its native state using amphipols, we have solved, by (1)H NMR, structure receptor-bound B4 (LTB4). Upon binding, LTB4 adopts highly...
Abstract Amphipols (APols) are amphiphatic polymers that keep membrane proteins (MPs) water‐soluble. The best characterized and most widely used APol to date, A8‐35, comprises a polyacrylate backbone grafted with octyl‐ isopropylamine side chains. nature of its hydrophilic moieties prevents use at the slightly acidic pH is desirable slow down rate amide proton exchange in solution NMR studies. We describe here synthesis properties pH‐insensitive APols obtained by replacing isopropyles...
Abstract Cell membranes represent a complex and variable medium in time space of lipids proteins. Their physico-chemical properties are determined by lipid components which can turn influence the biological function membranes. Here, we used hydrostatic pressure to study close dynamic relationships between membrane Experiments on β –barrel OmpX α –helical BLT2 G Protein-Coupled Receptor nanodiscs different compositions reveal conformational landscapes intimately linked lipids. Pressure modify...
Abstract G Protein-Coupled receptors represent the main communicating pathway for signals from outside to inside of most eukaryotic cells. They define largest family integral membrane at surface cells and constitute target current drugs on market. The low affinity leukotriene receptor BLT2 is a involved in pro- anti-inflammatory pathways can be activated by various unsaturated fatty acid compounds. We present here NMR structure agonist 12–HHT its BLT2-bound state model interaction ligand...
Tripartite efflux pumps built around ATP-binding cassette (ABC) transporters are membrane protein machineries that perform vectorial export of a large variety drugs and virulence factors from Gram negative bacteria, using ATP-hydrolysis as energy source. Determining the number ATP molecules consumed per transport cycle is essential to understanding efficiency substrate transport. Using reconstituted pump in mimic environment, we show MacAB-TolC Escherichia coli couples with high efficiency....
In an effort to provide overview of the biophysical approaches used study G-protein-coupled receptors, we chose consider adenosine A2A receptor as a model, it is widely reported in literature explore way GPCRs are studied nowadays. After brief introduction receptor, gathered descriptions various tools investigate pharmacology and structure receptor. We began by describing key developments which have led successful studies including cloning, expression purification A2A, subsequent...