A5042443469- Advanced NMR Techniques and Applications
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Solid-state spectroscopy and crystallography
- Muon and positron interactions and applications
- Lipid Membrane Structure and Behavior
- Atomic and Subatomic Physics Research
- Bacteriophages and microbial interactions
- NMR spectroscopy and applications
- Advanced MRI Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Receptor Mechanisms and Signaling
- thermodynamics and calorimetric analyses
- Machine Learning in Bioinformatics
- Geological Modeling and Analysis
- Neuropeptides and Animal Physiology
- Electron Spin Resonance Studies
- Molecular spectroscopy and chirality
- Microbial Metabolic Engineering and Bioproduction
- Magnetic and Electromagnetic Effects
- Geotechnical and construction materials studies
- Glycosylation and Glycoproteins Research
- Underground infrastructure and sustainability
Utrecht University
2010-2020
Institut de Pharmacologie et de Biologie Structurale
2005-2017
Université de Toulouse
2008-2017
Centre National de la Recherche Scientifique
2010-2017
Université Toulouse III - Paul Sabatier
2008-2017
Institut de Biologie Physico-Chimique
2014
Aix-Marseille Université
2013
Bruker (France)
2008
Laboratoire de Biotechnologie de l'Environnement
2008
Decrypting the structure, function, and molecular interactions of complex machines in their cellular context at atomic resolution is prime importance for understanding fundamental physiological processes. Nuclear magnetic resonance a well-established imaging method that can visualize entities micrometer scale be used to obtain 3D structures under vitro conditions. Here, we introduce solid-state NMR approach provides level insights into cell-associated components. By combining dedicated...
A peek inside: Dynamic nuclear polarization (DNP) enhances the spectroscopic sensitivity of solid-state NMR measurements uniformly (13C,15N)-labeled preparations Escherichia coli cells by more than an order magnitude (see picture; MW=microwaves, ε=enhancement factor). The major molecular components in can be characterized this way. Detailed facts importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made...
Cell walls are essential in the interaction of fungi with (a)biotic environment and also key to hyphal morphogenesis mechanical strength. Here, we used solid-state NMR (ssNMR) spectroscopy combined HPLC GC-MS study structural organization cell wall a representative Basidiomycota, one two main phyla fungi. Based on data propose refined model for basidiomycete. In this model, rigid core is built from α- β-(1,3)-glucan, β-(1,3)-(1,6)-glucan, highly branched single stranded β-(1,4)-chitin as...
Multidomain proteins constitute a large part of prokaryotic and eukaryotic proteomes play fundamental roles in various physiological processes. However, their structural characterization is challenging because size intrinsic flexibility. We show here that motional-filtered high-resolution solid-state NMR (ssNMR) experiments allow for the observation analysis very multidomain membrane are characterized by different motional time scales. This approach was used to probe folding 790-residue...
Was ist in der Zelle? Durch Anwendung dynamischen Kernpolarisation (DNP) wird die spektroskopische Empfindlichkeit von Festkörper-NMR-Messungen an einheitlich 13C,15N-markierten Proben Escherichia coli um mehr als eine Größenordnung verbessert (siehe Bild; MW=Mikrowellen, ε=Verstärkungsfaktor). Mit dieser Methode gelingt es, molekularen Hauptkomponenten Zelle zu analysieren.
High-Resolution Magic-Angle Spinning (HR-MAS) NMR spectroscopy has become an extremely versatile analytical tool to study heterogeneous systems endowed with liquid-like dynamics. frequencies of several kHz are however required obtain spectra, devoid spinning sidebands, a resolution approaching that purely isotropic liquid samples. An important limitation the method is large centrifugal forces can damage structure sample. In this communication, we show optimizing sample preparation,...
Significance Protein secretion is an essential determinant of bacterial physiology and virulence. Members the Corynebacteriales order have evolved a complex cell envelope containing two membranes, plasma membrane outer membrane, called mycomembrane, which harbors mycolic acids proteins (OMPs) unusual structure. Here, we investigated biogenesis OMPs in Corynebacterium glutamicum deciphered role O -mycoloylation targeting to mycomembrane. Partially enabled by our methodology, found that...
Abstract Biomolekulare Anwendungen der NMR‐Spektroskopie werden oft mit löslichen Molekülen oder Magnetresonanztomographie in Verbindung gebracht. Seit Ende 70er Jahre liefert darüber hinaus die Festkörper‐NMR‐Spektroskopie (FK‐NMR) auf atomarer Ebene Einblicke komplexe biomolekulare Systeme von Lipid‐Doppelschichten bis hin zu Biomaterialien. Im letzten Jahrzehnt haben Fortschritte im Bereich NMR‐Spektroskopie, Biophysik und Molekularbiologie das Repertoire FK‐NMR‐Spektroskopie für Studien...
Members of the histone-like nucleoid structuring protein (H-NS) family play roles both as architectural proteins and modulators gene expression in Gram-negative bacteria. The H-NS participates modulatory processes that respond to environmental changes osmolarity, pH, or temperature. oligomerization is essential for its activity. Structural models different truncated forms are available. However, high-resolution structural details full-length DNA-bound state have largely remained elusive. We...