A5012259975- Metabolomics and Mass Spectrometry Studies
- Neurological diseases and metabolism
- RNA regulation and disease
- Microbial Fuel Cells and Bioremediation
- RNA modifications and cancer
- Cancer-related gene regulation
- NMR spectroscopy and applications
- Analytical Chemistry and Chromatography
- Thyroid Cancer Diagnosis and Treatment
- Plant biochemistry and biosynthesis
- Microbial Community Ecology and Physiology
- Molecular spectroscopy and chirality
- Meat and Animal Product Quality
- Advanced Chemical Sensor Technologies
- Spectroscopy and Chemometric Analyses
- Cancer, Hypoxia, and Metabolism
- Traditional Chinese Medicine Studies
- Probiotics and Fermented Foods
- Biochemical effects in animals
- Fermentation and Sensory Analysis
- Proteins in Food Systems
- Wastewater Treatment and Nitrogen Removal
- Advanced MRI Techniques and Applications
- Bioinformatics and Genomic Networks
- Membrane-based Ion Separation Techniques
Centrale Marseille
2014-2023
Aix-Marseille Université
2007-2023
Centre National de la Recherche Scientifique
2012-2023
Institut des Sciences Moléculaires de Marseille
2012-2022
Université Claude Bernard Lyon 1
2009-2012
École Normale Supérieure de Lyon
2009-2012
Norwich Research Park
2009
Travaux et Recherches Archéologiques sur les Cultures, les Espaces et les Sociétés
2005-2007
Catalyse
2004
Mitochondrial respiration (oxidative phosphorylation, OXPHOS) is an emerging target in currently refractory cancers such as pancreatic ductal adenocarcinoma (PDAC). However, the variability of energetic metabolic adaptations between PDAC patients has not been assessed functional investigations. In this work, we demonstrate that OXPHOS rates are highly heterogeneous patient tumors, and high tumors enriched mitochondrial respiratory complex I at protein mRNA levels. Therefore, treated cells...
The metabo-ring initiative brought together five nuclear magnetic resonance instruments (NMR) and 11 different mass spectrometers with the objective of assessing reliability untargeted metabolomics approaches in obtaining comparable profiles. This was estimated by measuring proportion common spectral information extracted from LCMS NMR platforms. Biological samples obtained 2 conditions were analysed partners using their own in-house protocols. Test #1 examined urine adult volunteers either...
The world faces complex challenges for chemical hazard assessment. Microfluidic bioartificial organs enable the spatial and temporal control of cell growth biochemistry, critical organ-specific metabolic functions particularly relevant to testing dose–response signatures associated with both pharmaceutical environmental toxicity. Here we present an approach combining a microfluidic system 1H NMR-based metabolomic footprinting, as high-throughput small-molecule screening approach. We...
Significance testing is a crucial step in metabolic biomarker recovery from the metabolome-wide latent variables computed by multivariate statistical analysis. In this study we propose an algorithm based on landscape of covariance/correlation ratio consecutive along chemical shift axis to restore, prior significance testing, spectral dependency and recouple clusters which correspond physical, chemical, biological entities: recoupling (SRV). Variables are associated into series clusters, then...
The tetrameric cytoplasmic FeFe hydrogenase Hnd from Solidesulfovibrio fructosivorans (formely Desulfovibrio fructosovorans) catalyses H2 oxidation and couples the exergonic reduction of NAD+ to endergonic a ferredoxin by using flavin-based electron-bifurcating mechanism. Regarding its implication in bacterial physiology, we previously showed that Hnd, which is non-essential when bacteria grow fermentatively on pyruvate, involved ethanol metabolism. Under these conditions, it consumes...
We performed a preliminary NMR investigation on grated Parmigiano Reggiano cheese of different ripening ages. Principal component analysis (PCA) and discriminant (DA) were able to successfully group the analyzed samples according their respective Keywords: HRMAS NMR; cheese; chemometrics; PCA; DA
We tested the potential of High-Resolution MAS NMR spectroscopy to study 20 samples Emmental cheeses from 7 different geographical regions. Principal component analysis (PCA) and discriminant (DA) were used analyze data set 1H HRMAS spectra succeeded in grouping studied according their origins.
Cytological analysis of thyroid nodules detected using ultrasound-guided fine-needle aspiration technique is an efficient method for the diagnosis well-differenciated tumors such as papillary carcinoma. However, between 10 to 30% all nodules, cytological based on biopsies leads "indeterminated" identification. Consequently, a surgical excision then necessary definite histological lesions, resulting in 85% patient with indeterminated undergoing unnecessary surgery since their tumor finally...
We explored, using nuclear magnetic resonance (NMR) metabolomics and fatty acids profiling, the effects of a common nutritional complement, Curcuma longa, at nutritionally relevant dose with human use, administered in conjunction an unbalanced diet. Indeed, traditional food supplements have been long used to counter metabolic impairments induced by diets. Here, rats were fed either standard diet, high level fructose saturated acid (HFS) diet western countries that certainly contributes...
Breast cancer (BC) is the most common form of among women worldwide. Despite huge advancements in its treatment, exact etiology breast still remains unresolved. There an increasing interest role gut microbiome modulating anti-cancer therapeutic response. It seems that alteration microbiome-derived metabolome potentially promotes carcinogenesis. Taken together, metabolomics has arisen as a fascinating new omics field to screen promising metabolic biomarkers. In this study, fecal metabolite...
The development of Statistical Total Correlation Spectroscopy (STOCSY), a representation the autocorrelation matrix spectral data set as 2D pseudospectrum, has allowed more reliable assignment one- and two-dimensional NMR spectra acquired from complex mixtures that are usually used in metabolomics/metabonomics studies, thus, improving precise identification candidate biomarkers contained metabolic signatures computed by multivariate statistical analysis. However, correlations obtained cannot...
Objective In the management of patients with IBD, there is a need to identify prognostic markers and druggable biological pathways improve mucosal repair probe efficacy tumour necrosis factor alpha biologics. Vnn1 pantetheinase that degrades pantetheine pantothenate (vitamin B 5 , precursor coenzyme A (CoA) biosynthesis) cysteamine. overexpressed by inflamed colonocytes. We investigated its contribution tolerance intestinal mucosa colitis-induced injury. Design performed an RNA sequencing...
Abstract High‐resolution magic angle spinning (HRMAS) NMR is probably the most apt method to analyze complex materials involving a solid phase, e.g. foodstuffs. We present here an HRMAS analysis of grated cheese (Parmigiano Reggiano). A full characterization this allows identification presence fatty acids (saturated and unsaturated), amino other small organic molecules. Since relative concentration these molecules have previously been shown correlate with organoleptic, origin age...
High-Resolution Magic-Angle Spinning (HR-MAS) NMR spectroscopy has become an extremely versatile analytical tool to study heterogeneous systems endowed with liquid-like dynamics. frequencies of several kHz are however required obtain spectra, devoid spinning sidebands, a resolution approaching that purely isotropic liquid samples. An important limitation the method is large centrifugal forces can damage structure sample. In this communication, we show optimizing sample preparation,...
High-resolution magic-angle spinning (HR-MAS) nuclear magnetic resonance (NMR) is an essential tool to characterize a variety of semisolid systems, including biological tissues, with virtually no sample preparation. The "non-destructive" nature NMR typically compromised, however, by the extreme centrifugal forces experienced under conventional HR-MAS frequencies several kilohertz. These features limit usefulness current approaches for fragile samples. Here, we introduce full protocol...
Like other tumors, aggressive soft tissue sarcomas (STS) use glycolysis rather than mitochondrial oxidative phosphorylation (OXPHOS) for growth. Given the importance of cofactor coenzyme A (CoA) in energy metabolism, we investigated impact Vnn1 pantetheinase-an enzyme that degrades pantetheine into pantothenate (vitamin B5, CoA biosynthetic precursor) and cysyteamine-on tumor Using two models, show Vnn1+ STS remain differentiated grow slowly, patients a detectable level VNN1 expression is...
The metabolic effects of an oral supplementation with a Curcuma longa extract, at dose nutritionally relevant common human use, on hepatic metabolism in rats fed high fructose and saturated fatty acid (HFS) diet was evaluated. High-resolution magic-angle spinning NMR GC/MS combination multivariate analysis have been employed to characterize the metabolite profiles composition liver tissue respectively. results showed clear discrimination between HFS groups controls involving metabolites such...
We review the progress and state-of-the-art applications of studies in Magnetic Resonance Spectroscopy (MRS) Imaging as an aid for diagnosis thyroid lesions different nature, especially focusing our attention to those that are cytologically undetermined. It appears high-resolution High-Resolution Magic-Angle-Spinning (HRMAS) MRS improves overall accuracy analysis a point significant improvement undetermined can be expected. This analysis, meantime, allows more precise comprehension...
Deep-sea hydrothermal vents are extreme and complex ecosystems based on a trophic chain. We still unsure of the identities first colonizers these environments their metabolism, but they thought to be (hyper)thermophilic autotrophs. Here we investigate whether electric potential observed across chimneys could serve as an energy source for colonizers. Experiments were performed in two-chamber microbial electrochemical system inoculated with deep-sea chimney samples, cathode sole electron...
In this paper we present a general approach for the blind identification of compounds from solutions using NMR spectroscopy and source separation algorithms.