A5109242940- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Ion-surface interactions and analysis
- Analytical chemistry methods development
- Advanced Chemical Sensor Technologies
- Advanced Chemical Physics Studies
- DNA and Nucleic Acid Chemistry
- Advanced Proteomics Techniques and Applications
- Pesticide Residue Analysis and Safety
- Atomic and Molecular Physics
- Microbial Natural Products and Biosynthesis
- Molecular Sensors and Ion Detection
- Chemical Synthesis and Analysis
- RNA and protein synthesis mechanisms
- Microfluidic and Capillary Electrophoresis Applications
- Head and Neck Cancer Studies
- Chemical Reaction Mechanisms
- Catalytic Alkyne Reactions
- Isotope Analysis in Ecology
- Lipid Membrane Structure and Behavior
- Metal-Organic Frameworks: Synthesis and Applications
- Synthetic Organic Chemistry Methods
- Estrogen and related hormone effects
- Molecular spectroscopy and chirality
Centre Hospitalier de l’Université de Montréal
2009-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2008-2024
CEA Paris-Saclay
2008-2024
Institut Parisien de Chimie Moléculaire
2013-2024
Sorbonne Université
2014-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Université Paris-Saclay
2008-2024
Institut de Biologie et Technologies
2008-2024
Technologies pour la Santé
2019-2024
Hôpital Maisonneuve-Rosemont
2022
In this study, we show that liquid chromatography coupled with tandem mass spectrometry provides a sensitive, specific, and accurate absolute quantification of Erbitux, human:murine chimeric mAb used for the treatment colorectal cancer. Micrometric magnetized beads, functionalized soluble epidermal growth factor receptor (sEGFR), pharmacological target were specific immunocapture Erbitux allowing assessment antibody's biological potency sample purification. Following digestion trypsin,...
The metabo-ring initiative brought together five nuclear magnetic resonance instruments (NMR) and 11 different mass spectrometers with the objective of assessing reliability untargeted metabolomics approaches in obtaining comparable profiles. This was estimated by measuring proportion common spectral information extracted from LCMS NMR platforms. Biological samples obtained 2 conditions were analysed partners using their own in-house protocols. Test #1 examined urine adult volunteers either...
Metabolic profiles of biofluids obtained by atmospheric pressure ionization mass spectrometry-based technologies contain hundreds to thousands features, most them remaining unknown or at least not characterized in analytical systems. We report here on the annotation human adult urinary metabolome and metabolite identification from electrospray spectrometry (ESI-MS)-based metabolomics data sets. Features biological interest were first all annotated using ESI-MS database laboratory. They also...
Microcin E492 (MccE492, 7886 Da), the 84-amino acid antimicrobial peptide from Klebsiella pneumoniae, was purified in a post-translationally modified form, MccE492m (8717 culture supernatants of either recombinant Escherichia coli VCS257 strain harboring pJAM229 plasmid or K. pneumoniae RYC492 strain. Chymotrypsin digestion led to MccE492m-(74–84) C-terminal fragment that carries modification and analyzed by mass spectrometry nuclear magnetic resonance at natural abundance. The 831-Da...
Metabolomics is considered as an emerging new tool for functional proteomics in the identification of protein function or projects aiming at modeling whole cell metabolism. When combined with proteome studies, metabolite-profiling analyses revealed unanticipated insights into yeast sulfur pathway. In response to cadmium, observed overproduction glutathione, essential detoxification metal, can be entirely accounted by a marked drop sulfur-containing synthesis and redirection metabolite fluxes...
Ester-way to heaven: Unexpected formation of bicyclo[3.1.0]hexene 4 was the main focus combined experimental and theoretical studies on Au-catalyzed cycloisomerization branched dienyne 1 (see scheme), which provided better understanding mechanistic details governing cyclization enynes bearing a propargylic ester group.Experimental Au- Pt-catalyzed with an acetate group at position are presented. The peculiar architecture precursor, has both 1,6- 1,5-enyne skeleton, leads, in presence...
Recently, high-resolution mass spectrometry has been largely employed for compound identification, thanks to accurate measurements. As additional information, relative isotope abundance (RIA) is often needed reduce the number of candidates prior tandem MSn. Here, we report on evaluation LTQ-Orbitrap, in terms and RIA measurements building further metabolomics spectral databases. Accurate were achieved ppm range, using external calibration within 24 h, remained at <5 over a one-week period....
The intermediacy of gold acetylides in the gold(I)-catalyzed cycloisomerization enynes was questioned. While dinuclear complexes are observed under electrospray ionization conditions, solution reactivity also leads to conclusion their high affinity for second coordination a moiety, leading complexes. However, involvement and corresponding diaurated species elementary steps mechanisms appears unlikely.
We report the direct introduction of biological samples into a high-resolution mass spectrometer, LTQ-Orbitrap, as fast tool for metabolomic studies. A proof concept study was performed on yeast cell extracts that were introduced spectrometer by using flow injection analysis, with an acquisition time 3 min. Typical spectra contained few thousand m/z signals, 400 which found to be analytically relevant (i.e., their intensity 3-fold higher than background noise and they occurred in at least...
Metabolomics, i.e., the global analysis of cellular metabolites, is becoming a powerful tool for gaining insights into biological functions in postgenomic context. However, absolute quantitation endogenous metabolites media remains an issue, and available technologies metabolome still lack robustness accuracy. We describe here new method based on liquid chromatography−mass spectrometry 15N uniform metabolic labeling Saccharomyces cerevisiae accurate nitrogen-containing cell profiling...
A strategy combining autocorrelation matrices and ultrahigh resolution mass spectrometry (MS) was developed to optimize the characterization of discriminating ions highlighted by metabolomics. As an example, urine samples from rats treated with phenobarbital (PB) were analyzed ultrahigh-pressure chromatography two different eluting conditions coupled time-of-flight spectrometric detection in both positive negative electrospray ionization modes. Multivariate data analyses performed highlight...
A simple and fast selective extraction of the antibiotic chloramphenicol (CAP) from milk (raw milk, skimmed powder) using a molecularly imprinted polymer (MIP) sorbent is described. The method entails single centrifugation step prior to loading supernatant onto MIP cartridge subsequent elution with mixture solvents. CAP was further analyzed by isotope dilution liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) operating in negative acquisition mode....
We present experimental results for proton ionization of nucleobases (adenine, cytosine, thymine, and uracil) based on an event-by-event analysis the different ions produced combined with absolute target density determination. are able to disentangle in detail various channels from mass-analyzed product ion signals coincidence charge-analyzed projectile. In addition we determine a complete set cross sections these molecular targets by 20--150 keV protons including total partial...
Apelin peptides were recently identified as endogenous ligands of the APJ receptor. It has been hypothesized that these are initially provided to newborn by nursing and might be involved in gastrointestinal tract development. As apelin may have different effects on receptor a function their size, knowledge exact structure early milk is essential clarify action Bovine colostrum thought contain high concentrations wide diversity peptides, but none them yet rigorously characterized. To identify...
Bis-quinacridine cyclo-bis-intercaland receptors are shown to form 1:2 complexes with nucleoside monophosphates in water (see scheme). The preferential binding of guanosine derivatives, which might originate specific base/base interactions inside the complex, has been confirmed by ESI-MS experiments.
Abstract This work reports the use of reverse‐phase liquid chromatography coupled to electrospray ion trap (QIT) mass spectrometry for analysis metabolome in rat urine. An injection 20 μL urine into chromatographic system is followed by a slow gradient elution and spectrometric detection scanning mode from m/z 100–1000 both positive negative modes. Over time scale 90 min, 30 resolved peaks were observed modes, respectively, corresponding presence few hundred ratios. By using QIT analyzer,...
A convenient way to study lipid oxidation products-modified proteins by means of suitable model systems has been investigated. As a peptide, the oxidized B chain insulin chemically modified either 4-hydroxy-2-nonenal (HNE) or hexanal and extent, sites, structure modifications were assessed electrospray mass spectrometry. reduction step, using NaCNBH(3) NaBH(4), was also studied stabilize alkylated compounds. From data gathered, it appeared that NaCNBH(3), when added at beginning incubation,...
Mass analyzed product ions have been detected in coincidence with the projectile following ionization of water by proton impact. Measurement charge state postcollision enables different processes to be identified: direct ionization, single electron capture, and double capture. A complete set partial total absolute cross sections is reported for capture initiated collisions at 20--150 keV. The ${\mathrm{H}}_{2}\mathrm{O}$ impact are compared previous results [Straub et al., J. Chem. Phys....
Abstract Internal energies and energy distributions were studied using the ‘survival yield’ method developed previously. In addition to conventional benzylpyridinium salts, protonated esters (fragmenting by rearrangement) leucine enkephalin also used, extending validity of technique. Fragmentation processes in cone voltage region modeled RRKM‐based MassKinetics program. The results show that shapes are similar thermal distributions. mean internal very for all compound classes studied, a...
The incorporation of lanthanides into polyoxometalates provides entry to new classes potentially useful materials that combine the intrinsic properties both constituents. To utilize [α1-Ln(H2O)4P2W17O61]7- species in applications catalysis and development luminescent materials, chemistry this family lanthanide organic solvents has been developed. Organic-soluble polyoxometalate−lanthanide complexes TBA5H2[α1-Ln(H2O)4P2W17O61] (Ln = La(III), Sm(III), Eu(III), Yb(III)) were prepared...