A5083995281- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- RNA Interference and Gene Delivery
- Chromosomal and Genetic Variations
- Advanced biosensing and bioanalysis techniques
- Virology and Viral Diseases
- Cytomegalovirus and herpesvirus research
- Plant Virus Research Studies
- Advanced Fluorescence Microscopy Techniques
- RNA modifications and cancer
- RNA Research and Splicing
- ATP Synthase and ATPases Research
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Solid-state spectroscopy and crystallography
- Bacterial Genetics and Biotechnology
- Advanced Biosensing Techniques and Applications
- Lipid Membrane Structure and Behavior
- Advanced NMR Techniques and Applications
- interferon and immune responses
- Molecular spectroscopy and chirality
Institut de Chimie des Substances Naturelles
2013-2023
Centre National de la Recherche Scientifique
2011-2022
Université Paris-Saclay
2015-2022
Université Paris-Sud
2016-2020
Délégation Paris 5
1997-2014
Université Paris Cité
2006-2014
Inserm
2001-2011
Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques
2011
Wabash College
2009
Institut de Chimie Moléculaire de Paris : organique, inorganique et biologique
2009
This paper presents Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST), a small monomeric protein tag, half as large the green fluorescent protein, enabling labeling of proteins in reversible specific manner through binding activation cell-permeant nontoxic fluorogenic ligand (a so-called fluorogen). A unique fluorogen mechanism based on two spectroscopic changes, increase fluorescence quantum yield absorption red shift, provides high selectivity. Y-FAST was engineered from...
Mapping the conformational landscape of G protein-coupled receptors (GPCRs), and in particular how this is modulated by membrane environment, required to gain a clear picture signaling proceeds. To end, we have developed an original strategy based on solution-state nuclear magnetic resonance combined with efficient isotope labeling scheme. This was applied typical GPCR, leukotriene B4 receptor BLT2, reconstituted lipid bilayer. Because this, are able provide direct evidence that BLT2...
The critical functions of the HIV-1 nucleocapsid protein NCp7 in genomic RNA packaging and reverse transcription, essentially rely on interactions with nucleic acids. A significant progress knowledge these has been recently achieved NMR-derived structures derivatives complex two short sequences ψ signal, namely ACGCC stem-loop 3 (SL3) motif. To further identify key nucleotides formation both NCp7−d(ACGCC) NCp7−SL3 complexes, we quantitatively analyzed by steady-state time-resolved...
The HIV-1 nucleocapsid protein, NCp7, is characterized by two CCHC zinc finger motifs which have been shown to stoichiometrically bind in mature virions. Moreover, this binding of proves be critical various NCp7 functions, especially the encapsidation process. To further understand central role we closely investigated properties and deleted or substituted derivatives. end, fluorescence either naturally occurring Trp37 conservatively Trp16 was used monitor N- C-terminal motifs, respectively....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT1H NMR structure and biological studies of the His23 .fwdarw. Cys mutant nucleocapsid protein HIV-1 indicate that conformation first zinc finger is critical for virus infectivityH. Demene, C. Z. Dong, M. Ottmann, Rouyez, N. Jullian, Morellet, Y. Mely, J. L. Darlix, Fournie-Zaluski, Cite this: Biochemistry 1994, 33, 39, 11707–11716Publication Date (Print):October 4, 1994Publication History Published online1 May 2002Published inissue 4 October...
Gag protein oligomerization, an essential step during virus assembly, results in budding of spherical particles. This process is critically dependent on the spacer p2, located between capsid and nucleocapsid proteins. P2 contributes also, association with NCp7, to specific recognition HIV-1 packaging signal resulting viral genome encapsidation. There no structural information about 20 last amino acids C-terminal part (CA[CTD]) molecular mechanism assembly. In this study structure a peptide...
Double-stranded RNA (dsRNA) virions constitute transcriptionally competent machines that must translocate across cell membranes to function within the cytoplasm. The entry mechanism of such non-enveloped viruses is not well described. Birnaviruses are unique among dsRNA because they possess a single shell for entry. We hereby report how infectious bursal disease virus, an avian birnavirus, can disrupt and enter into its target cells. One four structural peptides, pep46 (a 46-amino acid...
The plant IRT1 iron transporter is a plasma membrane protein that takes up in root upon iron-limited conditions. Besides its primary metal substrate iron, transports other divalent metals overaccumulate plants when soil low and highly expressed. We previously reported the intracellular regulatory loop between transmembrane helices TM4 TM5 involved post-translational regulation of by non-iron substrates. Upon excess zinc, undergoes phosphorylation CIPK23 followed ubiquitination IDF1 to target...
Non-natural amino acids are important tools for site-selective probing of peptide properties and interactions. Here, the first time a fluorescent l-amino acid, exhibiting excited-state intramolecular proton transfer (ESIPT) hydration-sensitive dual emission, was synthesized. It is an analogue l-tryptophan bearing slightly larger 2-(2-furyl)-3-hydroxychromone aromatic moiety instead indole. This new acid incorporated through solid-phase synthesis into NC(11–55), zinc finger domain HIV-1...
The human immunodeficiency virus type 1, HIV‐1, genome encodes a highly conserved regulatory gene product, Vpr (96 amino acids), which is incorporated into virions in quantities equivalent to those of the viral Gag protein. In infected cells, believed function during early stages HIV‐1 replication (such as transcription proviral and migration preintegration nuclear complex), blocks cells G2 phase triggers apoptosis. also plays critical role long‐term AIDS disease by inducing infection...
Abstract Background The human immunodeficiency virus type 1 (HIV-1) encodes several regulatory proteins, notably Vpr which influences the survival of infected cells by causing a G2/M arrest and apoptosis. Such an important role in HIV-1 disease progression has fuelled large number studies, from its 3D structure to characterization specific cellular partners. However, no direct imaging quantification Vpr-Vpr interaction living yet been reported. To address this issue, eGFP- mCherry proteins...
The piggyBac transposase (PB) is distinguished by its activity and utility in genome engineering, especially humans where it has highly promising therapeutic potential. Little known, however, about the structure–function relationships of different domains PB. Here, we demonstrate vitro vivo that C-terminal Cysteine-Rich Domain (CRD) essential for DNA breakage, joining transposition binds to specific sequences left right transposon ends, an additional unexpectedly internal site at end. Using...
New Delhi metallo-β-lactamase-1 (NDM-1) has recently emerged as a global threat because of its ability to confer resistance all common β-lactam antibiotics. Understanding the molecular basis hydrolysis by NDM is crucial for designing inhibitors or β-lactams resistant their hydrolysis. In this study, first time, NMR was used study influence Zn(II) ions on dynamic behavior NDM-1. Our results highlighted that binding in NDM-1 active site induced several structural and changes loop 2 (ASL2) L9...
The two highly conserved Zn2+ finger motifs of the HIV-1 nucleocapsid protein, NCp7, strongly bind through coordination one His and three Cys residues. To further analyze role these residues, we investigated binding acid−base properties four single-point mutants a short peptide corresponding to distal motif NCp7. In each mutant, Zn2+-coordinating residue is substituted with noncoordinating one. Using spectroscopic Co2+, first establish that retain their ability metal cation four- or...