A5110337730- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Neuroscience and Neural Engineering
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- Advanced biosensing and bioanalysis techniques
- Photonic and Optical Devices
- Surface and Thin Film Phenomena
- Electrochemical sensors and biosensors
- Photonic Crystals and Applications
- Conducting polymers and applications
- Advanced Sensor and Energy Harvesting Materials
- Semiconductor materials and interfaces
- Nanofabrication and Lithography Techniques
- Electrochemical Analysis and Applications
- Molecular Junctions and Nanostructures
- Laser-Matter Interactions and Applications
- Quantum optics and atomic interactions
- Quantum and electron transport phenomena
- Force Microscopy Techniques and Applications
Beijing Academy of Science and Technology
2025
Hong Kong University of Science and Technology
2025
University of Hong Kong
2025
Xijing Hospital
2025
Air Force Medical University
2025
École Normale Supérieure - PSL
2015-2024
Université Paris Sciences et Lettres
2015-2024
Sorbonne Université
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Second Military Medical University
2024
This paper presents Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST), a small monomeric protein tag, half as large the green fluorescent protein, enabling labeling of proteins in reversible specific manner through binding activation cell-permeant nontoxic fluorogenic ligand (a so-called fluorogen). A unique fluorogen mechanism based on two spectroscopic changes, increase fluorescence quantum yield absorption red shift, provides high selectivity. Y-FAST was engineered from...
By choosing a material that has an appropriate asymmetric lattice mismatch to the host substrate, in this case ErSi2 on Si(001), it is possible grow one-dimensional epitaxial crystals. nanowires are less than one nanometer high, few nanometers wide, close micron long, crystallographically aligned 〈110〉Si directions, straight, and atomically regular.
Capture of circulating tumor cells (CTCs) from peripheral blood cancer patients has major implications for metastatic detection and therapy analyses. Here we demonstrated a microfluidic device high efficiency purity capture CTCs. The key novelty this approach lies on the integration microfilter with conical-shaped holes micro-injector cross-flow components size dependent without significant retention non-tumor cells. Under conditions constant flow rate, spiked into phosphate buffered saline...
Functional cellular substrates for localized cell stimulation by small molecules provide an opportunity to control and monitor signalling networks chemically in time space. However, despite improvements the controlled delivery of bioactive compounds, precise localization gaseous biomolecules at single-cell level remains challenging. Here we target nitric oxide, a crucial molecule with site-specific concentration-dependent activities, report synthetic strategy developing spatiotemporally...
High-purity cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are promising for drug development and myocardial regeneration. However, most hiPSC-derived CMs morphologically functionally resemble immature rather than adult CMs, which could hamper their application. Here, we obtained high-quality cardiac tissue-like constructs (CTLCs) by cultivating hiPSC-CMs on low-thickness aligned nanofibers made of biodegradable poly(D,L-lactic-co-glycolic acid) polymer. We...
Integrated Heart/Cancer on a Chip (iHCC) is promising microfluidic platform that allows the culture of different cell types separately and application closed-medium circulation to reproduce side effects doxorubicin heart <italic>in vitro</italic>.
Bacterial cellulose (BC) membranes with shape-memory properties allow the rapid preparation of artificial small-diameter blood vessels when combined microfluidics-based patterning multiple types cells. Lyophilization a wet multilayered rolled BC tube endows it memory to recover its tubular shape after unrolling. The unrolling yields flat membrane, and subsequent endothelial cells, smooth muscle fibroblast cells is carried out by microfluidics. cell-laden membrane then rerolled into tube....
We report a simple but straightforward approach to produce nanofiber scaffolds with incorporated protein gradient for cell culture studies. Standard electrospinning technique was used fabricate high-porosity random fiber matrix. Protein molecules were then deposited in the matrix by controlled filling method, allowing generation of concentration on sample. When cultured NIH 3T3 cells, it found that population depends strongly concentration. The morphology observation also showed effect...
Coulomb counting method is a convenient and straightforward approach for estimating the state-of-charge (SOC) of lithium-ion batteries. Without interrupting power supply, remaining capacities them in an electric vehicle (EV) can be calculated by integrating current leaving entering The main drawbacks this are cumulative errors time-varying coulombic efficiency, which always lead to inaccurate estimations. To deal with problem, least-squares based coulomb proposed. With proposed method,...
We report the first experimental investigation of spectral response a "bulk microcavity": semiconductor system in which same cavity material is active medium. Despite smaller oscillator strength bulk exciton, Rabi splitting even clearer than that displayed by usual quantum-well microcavities observed. The increases with layer thickness and exciton dispersion produces satellite structure due to quantum confinement. also show control exciton-photon interaction can be realized suitably...
Extensive efforts have been devoted to develop new substrates for culture and differentiation of human induced pluripotent stem cells (hiPSCs) toward cardiac cell-based assays. A more exciting prospect is the construction tissue robust drug screening repairing. Here, we developed a patch method by electrospinning crosslinking monolayer gelatin nanofibers on honeycomb frame made poly(ethylene glycol) diacrylate (PEGDA). The nanofibrous structure can support with minimal exogenous contact...
The field of intestinal biology is thirstily searching for different culture methods that complement the limitations organoids, particularly lack a differentiated compartment. While being recognized as an important milestone basic and translational biological studies, many primary cultures epithelium (IE) rely on empirical trials using hydrogels various stiffness, whose mechanical impact epithelial organization remains vague until now. Here, we report development hydrogel scaffolds with...
Heat stress (HS) at the reproductive stage detrimentally affects crop yields and seed quality. However, molecular mechanisms that protect processes in plants under HS remain largely unknown. Here, we report Acetylation Lowers Binding Affinity 3 (ALBA3) is crucial for safeguarding male fertility against Arabidopsis. ALBA3 highly expressed pollen, localized cytoplasm of both sperm vegetative cells. Mutants lacking functional exhibit hypersensitivity to HS, with reduced silique length due...