A5045168236- Tissue Engineering and Regenerative Medicine
- Cardiac Structural Anomalies and Repair
- Mechanical Circulatory Support Devices
- Cardiac Valve Diseases and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Cardiovascular Function and Risk Factors
- Infective Endocarditis Diagnosis and Management
- Xenotransplantation and immune response
- Transplantation: Methods and Outcomes
- Syntax, Semantics, Linguistic Variation
- CRISPR and Genetic Engineering
- Aortic Disease and Treatment Approaches
- Cardiac Imaging and Diagnostics
- Cardiac Fibrosis and Remodeling
- Cardiac Ischemia and Reperfusion
- Cardiac Arrest and Resuscitation
- Cardiac pacing and defibrillation studies
- 3D Printing in Biomedical Research
- Congenital Heart Disease Studies
- Congenital heart defects research
- Organ Transplantation Techniques and Outcomes
- Cardiac and Coronary Surgery Techniques
- Natural Language Processing Techniques
Osaka University
2016-2025
Universidade de São Paulo
2022-2025
Massachusetts Institute of Technology
2013-2025
Shizuoka Children's Hospital
2025
Seikei University
2023-2025
Osaka University Hospital
2012-2024
Osaka University of Human Sciences
2024
Osaka Gakuin University
2023
University of New Orleans
2022
Suita Municipal Hospital
2021
Background— Human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) are a promising source of cells for regenerating myocardium. However, several issues, especially the large-scale preparation hiPS-CMs and elimination undifferentiated iPS cells, must be resolved before hiPS can used clinically. The cell-sheet technique is one useful methods transplanting large numbers cells. We hypothesized that hiPS-CM-sheet transplantation would feasible, safe, therapeutically effective...
IL-6 is a multifunctional proinflammatory cytokine that elevated in the serum of patients with pulmonary arterial hypertension (PAH) and can predict survival idiopathic PAH (IPAH). Previous animal experiments clinical human studies indicate important PAH; however, molecular mechanisms IL-6-mediated pathogenesis have been elusive. Here we identified IL-21 as downstream target signaling PAH. First, found blockade by monoclonal anti-IL-6 receptor antibody, MR16-1, ameliorated hypoxia-induced...
Background. We hypothesized that tissue-engineered contractile cardiomyocyte sheets without a scaffold would show histological and electrical integration with impaired myocardium, leading to the regeneration of infarcted myocardium. Methods. Neonatal rat cardiomyocytes were cultured on Poly(N-isopropylacrylamide)-grafted polystyrene dishes detached as square cell sheet at 20°C. Two stacked make thicker cardiac sheets. In cross-section, looked like homogeneous heart-like tissue. weeks after...
Background— Transplantation of cardiomyocytes that are derived from human induced pluripotent stem cell–derived (hiPS-CMs) shows promise in generating new functional myocardium situ, whereas the survival and functionality transplanted cells critical considering this therapeutic impact. Cell-sheet method has been used to transplant many cells; however, potential ischemia might limit cell survival. The omentum, which is known have rich vasculature, expected be a source blood supply. We...
Cell therapy is a promising strategy for ischemic cardiomyopathy. However, the direct injection method has limitations generalized cell delivery, especially in dilated cardiomyopathy (DCM). We hypothesized that sheet-shaped myoblast graft would be superior to improving cardiac performance DCM.Male 27-week-old BIO TO-2 (DCM) hamsters showed moderate remodeling were used as recipients. Myoblasts isolated from F1B cultured on dishes coated with poly(N-isopropylacrylamide),...
Background When transplanted into failing heart, autologous somatic tissue–derived cells yield functional recovery via paracrine effects that enhance native regeneration. However, the therapeutic are modest. We developed a method in which scaffold‐free cell sheets attached to epicardial surface maximize effects. This Phase I clinical trial tested whether transplanting cell–sheets derived from skeletal muscle is feasible, safe, and effective for treating severe congestive heart failure....
In this study, we proposed that the functionality or phenotype of differentiated cardiomyocytes derived from human induced pluripotent stem cells (iPSC-CMs) might be modified by co-culture with mesenchymal (MSCs), resulting in an improved therapeutic potential for failing myocardial tissues. Structural, motility, electrophysiological, and metabolic analyses revealed iPSC-CMs co-cultured MSCs displayed aligned myofibrils A-, H-, I-bands could contract relax quickly, indicating promotion...
Background Atherosclerotic abdominal aortic aneurysm ( AAA ) is a progressive, gradual rupture that results in death the absence of surgical intervention. Key factors regulate initiation and progression are unknown, making targeted interventions difficult. Micro RNA s play fundamental role atherosclerosis, atherosclerotic coronary artery disease characterized by tissue‐ plasma‐specific micro signatures. However, little known about involved pathology. This study examined tissue plasma...
Background:Poor survival outcomes for patients with severe heart failure (HF) and the donor shortage transplantation warrant development of myocardial regenerative therapy. We performed a multicenter, phase II study to evaluate safety efficacy autologous skeletal myoblast sheets (TCD-51073).Methods Results:In 3 sites, we enrolled 7 chronic HF due ischemic disease despite maximal therapy, all whom underwent TCD-51073. No serious arrhythmia was reported, no changes were noted in frequency...
We hypothesized that autologous skeletal cell (SC) sheets regenerate the infract myocardium in porcine heart as a preclinical trial.The impaired was created by implantation of ameroid constrictor on left anterior descending for 4 weeks. SCs isolated from leg muscle were cultured and detached temperature-responsive domain-coated dishes single monolayer sheet at 20 degrees C. The following therapies conducted: SC (SC group, n=5); sham (C group n=5). Echocardiography demonstrated cardiac...
High-purity cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are promising for drug development and myocardial regeneration. However, most hiPSC-derived CMs morphologically functionally resemble immature rather than adult CMs, which could hamper their application. Here, we obtained high-quality cardiac tissue-like constructs (CTLCs) by cultivating hiPSC-CMs on low-thickness aligned nanofibers made of biodegradable poly(D,L-lactic-co-glycolic acid) polymer. We...