A5083808261- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Polymer Surface Interaction Studies
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Hydrogels: synthesis, properties, applications
- Additive Manufacturing and 3D Printing Technologies
- Nanoparticle-Based Drug Delivery
- biodegradable polymer synthesis and properties
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Biopolymer Synthesis and Applications
- Supramolecular Self-Assembly in Materials
- Nanofabrication and Lithography Techniques
- Advanced Polymer Synthesis and Characterization
- Nanoplatforms for cancer theranostics
- Silk-based biomaterials and applications
- Mesenchymal stem cell research
- Collagen: Extraction and Characterization
- Innovative Microfluidic and Catalytic Techniques Innovation
- Barrier Structure and Function Studies
- Adipose Tissue and Metabolism
- RNA Interference and Gene Delivery
- Dendrimers and Hyperbranched Polymers
- Graphene and Nanomaterials Applications
Osaka University
2016-2025
National Institute of Advanced Industrial Science and Technology
2023
National Institute of Health Sciences
2022
Museum of Japanese Art Yamato Bunkakan
2021
Suita Municipal Hospital
2010-2021
Division of Chemistry
2021
Japan Science and Technology Agency
2009-2019
Graduate School USA
2010-2019
Ube Frontier University
2018
Osaka Hospital
2014
A novel cell-accumulation technique has been developed using highly biocompatible nanofilms by layer-by-layer assembly for the rapid construction of thick layered tissues with a well-controlled layer number and thickness. Furthermore, 3D blood capillary networks (over 1 cm2 tissues) were also constructed sandwiching endothelial cells between tissues. Such simple methodology would be useful tissue engineering drug assessment. Detailed facts importance to specialist readers are published as...
Abstract With the current interest in cultured meat, mammalian cell-based meat has mostly been unstructured. There is thus still a high demand for artificial steak-like meat. We demonstrate vitro construction of engineered tissue assembled three types bovine cell fibers (muscle, fat, and vessel). Because actual an aligned assembly connected to tendon actions contraction relaxation, tendon-gel integrated bioprinting was developed construct tendon-like gels. In this study, total 72 comprising...
The preclinical drug screening of pancreatic cancer treatments suffers from the absence appropriate models capable to reproduce in vitro heterogeneous tumor microenvironment and its stiff desmoplasia. Driven by this pressing need, we describe paper conception characterization a novel 3D model consisting triple co-culture cells (PANC-1), fibroblasts (MRC-5) endothelial (HUVEC), which assembled form hetero-type multicellular spheroid (MCTS). By histological analyses Selective Plain...
Biodegradable hollow capsules encapsulating proteins were prepared via layer-by-layer assembly of chitosan and dextran sulfate on protein-entrapping mesoporous silica particles the subsequent removal silica. The enzymatic degradation in presence chitosanase was explored by scanning electron microscopy (SEM). With increasing time, component degraded chitosanase, began to deform finally destroyed. Sustained release encapsulated attained using capsules. behavior successfully manipulated...
Rapid and automatic development of three-dimensional (3D) human micro-tissue chips which integrate over 400 micro-arrays simplified multilayered structures is carried out using inkjet printing technology. Comprehensive high-throughput assays liver functions the 3D revealed highest a sandwich structure endothelial cells hepatocytes. These 3D-human tissue may enable "total-human models" for tailor-made drug screening. As service to our authors readers, this journal provides supporting...
Nanostructured gradient gels with unique bending properties due to their deswelling characteristics are presented. These readily fabricated via electrophoresis followed by photo-polymerization, and subsequent silica extraction. Differences in the physical between both sides of driving force behind gels. Supporting information for this article is available on WWW under http://www.wiley-vch.de/contents/jc_2089/2008/adma200702727_s.pdf or from author. Please note: The publisher not responsible...
Pack's drauf: Zu Zellmultischichten gelangt man über 6 nm dicke Filme extrazellulärer Matrix (ECM) aus Fibronectin (FN) und Gelatine auf der Oberfläche einer jeden Zellschicht. Die vierlagige Zellenarchitektur ist gut organisiert frei tragend. Xenogene Doppelschichtarchitekturen wie in menschlichen Blutgefäßen konnten durch Aufbringen Nanofilme Zelloberflächen hergestellt werden.
An in vitro drug-induced cardiotoxicity assay is a critical step drug discovery for clinical use. The use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) promising this purpose. However, single hiPSC-CMs are limited their ability to mimic native cardiac tissue structurally and functionally, the generation artificial using an ongoing challenging. We therefore developed new method constructing three-dimensional (3D) tissues short time by coating extracellular matrix...
Cartilage defects can result in pain, disability, and osteoarthritis. Hydrogels providing a chondroregeneration-permissive environment are often mechanically weak display poor lateral integration into the surrounding cartilage. This study develops visible-light responsive gelatin ink with enhanced interactions native tissue, potential for intraoperative bioprinting. A dual-functionalized tyramine methacryloyl (GelMA-Tyr) is synthesized. Photo-crosslinking of both groups triggered single...
Various nanometer-sized multilayers were directly prepared onto the surface of mouse L929 fibroblast cells by a layer-by-layer (LbL) assembly technique to control cell microenvironment and functions, such as viability, morphology, proliferation. The species LbL nanofilms strongly affected morphology growth. Polyelectrolyte (PE) induced round-shaped adhered cells, although each component had high cytocompatibility, whereas fibronectin (FN)−gelatin (G) −dextran sulfate (DS) with FN-binding...
In recent approaches to tissue engineering, cells face various stresses from physical, chemical, and environmental stimuli. For example, coating cell membranes with nanofilms using layer-by-layer (LbL) assembly requires many cycles of centrifugation, causing physical (gravity) stress. Damage can cause the leakage cytosol molecules or sometimes death. Accordingly, we evaluated effectiveness LbL films prepared on in protecting stresses. After two steps Tris-HCl buffer solution without polymers...
We developed a human skin equivalent (HSE) containing blood and lymph-like capillary networks using cell coating technique, which is rapid fabrication technology of three-dimensional cellular constructs by surface layer-by-layer assembled nanofilms extracellular matrices. The thickness dermis consisting normal dermal fibroblasts was easily controlled from approximately 5 to 100 µm altering the seeded number. Keratinocytes as major population showed homogeneous differentiation on lifting...
Bioinspired and adhesive multilayer membranes are produced using the layer-by-layer (LbL) assembly of chitosan (CHT), alginate (ALG) hyaluronic acid modified with dopamine (HA-DN). Freestanding without DN also as a control. The success synthesis HA-DN was confirmed UV-visible spectroscopy. Scanning electron microscopy images indicate that surface DN-containing is more porous than control ones; they present higher average thickness value for same number CHT/ALG/CHT/HA(-DN) tetralayers (n =...
Porphyromonas gingivalis is a major pathogen in severe and chronic manifestations of periodontal disease, which one the most common infections humans. A central feature P. pathogenicity dysregulation innate immunity at gingival epithelial interface; however, molecular basis underlying gingivalis–dependent abrogation barrier function remains unknown. Gingival cells express junctional adhesion molecule (JAM1), tight junction–associated protein, JAM1 homodimers regulate function. Here we show...
Microvasculature is essential for the exchange of gas and nutrient most tissues in our body. Some tissue structures such as meniscus presents spatially confined blood vessels adjacent to non-vascularized regions. In biofabrication, mimicking spatial distribution vascular components paramount, capillary ingrowth into can lead matrix alterations subsequent pathology. Multi-material three-dimensional (3D) bioprinting strategies have potential resolve anisotropic features, although building...
Since the current process of livestock meat production has considerable effects on global environment, leading to high emissions greenhouse gases, cultured recently attracted attention as a suitable alternative way acquire animal proteins. However, while most published studies cell-cultured have focused muscle tissue culture, fat which is an important component often been neglected from this technology, even though it can enhance meat's final taste, aroma, tenderness, texture, and...