A5065666549- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- 3D Printing in Biomedical Research
- Acoustic Wave Resonator Technologies
- Microtubule and mitosis dynamics
- Ferroelectric and Piezoelectric Materials
- Micro and Nano Robotics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced MEMS and NEMS Technologies
- Advanced Sensor and Energy Harvesting Materials
- Electrowetting and Microfluidic Technologies
- Powder Metallurgy Techniques and Materials
- Microbial Inactivation Methods
- Vibration and Dynamic Analysis
- Adhesion, Friction, and Surface Interactions
- Neuroscience and Neural Engineering
- Pluripotent Stem Cells Research
- Adaptive optics and wavefront sensing
- Advanced Materials and Mechanics
- Tissue Engineering and Regenerative Medicine
- Multiferroics and related materials
- Nanofabrication and Lithography Techniques
- Microwave Dielectric Ceramics Synthesis
- Pancreatic function and diabetes
- Modular Robots and Swarm Intelligence
Osaka University
2004-2023
Kyoto University
2011-2020
Kyoto Katsura Hospital
2013-2020
RIKEN
2018-2020
Nippon Soken (Japan)
2019-2020
Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin
2020
The University of Tokyo
2006-2015
Kobe University
2013
Japan Science and Technology Agency
2007-2012
Kagawa University
2011
Creating vascular networks in tissues is crucial for tissue engineering.
High-purity cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are promising for drug development and myocardial regeneration. However, most hiPSC-derived CMs morphologically functionally resemble immature rather than adult CMs, which could hamper their application. Here, we obtained high-quality cardiac tissue-like constructs (CTLCs) by cultivating hiPSC-CMs on low-thickness aligned nanofibers made of biodegradable poly(D,L-lactic-co-glycolic acid) polymer. We...
Micro-orifice based cell fusion assures high-yield without compromising the viability. This paper examines feasibility of a dielectrophoresis (DEP) assisted trapping method for parallel with micro-orifice array. The goal is to create viable fusants studying postfusion behavior. We fabricated microfluidic chip that contained chamber and partition. partition divided into two compartments it had number embedded micro-orifices. voltage applied electrodes located at each compartment generated an...
This paper presents a polymer-based micropump addressing the cost, performance, and system compatibility issues that have limited integration of on-chip micropumps into microanalysis systems. pump uses dielectric elastomer actuation to periodically displace fluid, pair elastomeric check valves rectify fluid's resulting movement. Its significant features include use transparent substrate, self-priming capability, insensitivity gas bubbles, ability admit particles. A occupying less than 10 mm2...
<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> In this paper, we report a piezoelectric deformable mirror composed of thin films for low-voltage adaptive optics (AO). A 2-<formula formulatype="inline"><tex>$\mu$</tex></formula>m-thick Pb(Zr,Ti)O<formula formulatype="inline"><tex>$_{3}$</tex></formula> (PZT) film was deposited on Pt-coated silicon-on-insulator (SOI) substrate, and diaphragm structure 15 mm in diameter fabricated by etching Si...
Ferroelectric PZT-based perovskite thin films are widely studied for fabrication of compact piezoelectric energy harvesting (EH) power microelectromechanical systems (MEMS) due to their large coefficients. Output the EH MEMS is chiefly governed by conversion rate, κ <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> and/or (e /ε), where e and ε denote coefficient dielectric constant. The values /ε) considered as figures merit (FOM) MEMS. At...
We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic (cytRNA) from single cell enables single-cell integrated nucRNA cytRNA-sequencing (SINC-seq). SINC-seq constructs two individual RNA-seq libraries, cytRNA, per cell, quantifies gene expression in the subcellular compartments, combines them to create novel data. Leveraging SINC-seq, we discover distinct natures of correlation among cytRNA reflect transient physiological state cells. These data...
The simultaneous analysis of RNA and DNA single cells remains a challenge as these species have very similar physical biochemical properties can cross-contaminate each other. Presented is an on-chip system that enables selective lysing living cells, extraction, focusing, absolute quantification cytoplasmic mass its separation from in the nucleus using electrical isotachophoresis (ITP). This quantitation performed without enzymatic amplification less than 5 min. preserved, fluorescence signal...
Current in vitro 3D culture models lack a vascular system to transport oxygen and nutrients, as well cells, which is essential maintain cellular viability functions. Here, we describe microfluidic method generate perfusable network that can form inside multicellular spheroids functionally connect microchannels. Multicellular containing endothelial cells lung fibroblasts were embedded within hydrogel microchannel, then, seeded into both sides of the so angiogenic sprouts from cell...
Crystallographic structure of as-grown epitaxial Pb(Zr,Ti)O3 (PZT) films was investigated with regard to the Zr/Ti ratio and crystalline orientation. PZT (001) (111) orientation were epitaxially grown on (100) (111)SrTiO3 substrates respectively using radio-frequency (rf) sputtering. Four circle x-ray diffraction measurements revealed that crystallographic dependence composition in much different from bulk PZT. In particular, (001)-oriented showed tetragonal even 70/30 where ceramics are...
The field of microfluidics has drastically contributed to downscale the size benchtop experiments dimensions a chip without compromising results. However, further miniaturization and ability directly manipulate individual molecules require platform that permits organized molecular transport. motor proteins microtubules carry out orderly intracellular transport are ideal for driving in vitro nanotransport. Here, we demonstrate reconstruction cellular kinesin/dynein-microtubule system...
Abstract In this paper, we present a novel electrofusion device that enables massive parallelism, using an electrically insulating sheet having two‐dimensional micro‐orifice array. The is sandwiched by pair of micro‐chambers with immersed electrodes, and each chamber filled the suspensions two types cells to be fused. Dielectrophoresis, assisted sedimentation, used position in upper down onto orifices, then flipped over on other side, so cell pairs making contact orifice are formed. When...
Thermodynamic characteristics of single crystalline $\mathrm{Pb}({\mathrm{Zr}}_{0.52}{\mathrm{Ti}}_{0.48}){\mathrm{O}}_{3}$ (PZT) were investigated using c-axis oriented PZT films. The films epitaxially grown on Pt/MgO substrate and dielectric ferroelectric properties measured as a function one-dimensional stress. stress dependence was examined the basis Landau-Devonshire's phenomenological theory free energy coefficients obtained. stiffness electrostrictive coefficient epitaxial obtained to...
A device is developed for low-voltage electroporation using field constriction at a micro-orifice, and the application to real-time measurement of single cell response demonstrated. The consists pair electrodes separated by an insulator film having regularly arranged array micro-fabricated orifices with typical diameter 1?2 ?m. Cells are immobilized aspiration, pulse voltage applied. lines, being unable penetrate insulator, go into create constriction. This means that most drop occurs in...