A5081075576- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- 3D Printing in Biomedical Research
- Microtubule and mitosis dynamics
- Force Microscopy Techniques and Applications
- Skin and Cellular Biology Research
- Cell Adhesion Molecules Research
- Micro and Nano Robotics
- Muscle Physiology and Disorders
- Biocrusts and Microbial Ecology
- Axon Guidance and Neuronal Signaling
- Nerve injury and regeneration
- Cancer-related gene regulation
- Cancer Cells and Metastasis
- Microfluidic and Bio-sensing Technologies
- Erythrocyte Function and Pathophysiology
- Tendon Structure and Treatment
- Hippo pathway signaling and YAP/TAZ
- Fibroblast Growth Factor Research
- RNA Research and Splicing
- Barrier Structure and Function Studies
- Spaceflight effects on biology
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Developmental Biology and Gene Regulation
Institut Jacques Monod
2016-2025
Université Paris Cité
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Délégation Paris 6
2015-2020
Délégation Paris 7
2013-2019
Canadian Nautical Research Society
2019
Sorbonne Université
2005-2016
Sorbonne Paris Cité
2016
National University of Singapore
2015
Singapore-MIT Alliance for Research and Technology
2015
Matrix rigidity sensing regulates a large variety of cellular processes and has important implications for tissue development disease. However, how cells probe matrix rigidity, hence respond to it, remains unclear. Here, we show that adaptation emerge naturally from actin cytoskeleton remodelling. Our in vitro experiments theoretical modelling demonstrate biphasic rheology the cytoskeleton, which transitions fluid on soft substrates solid stiffer ones. Furthermore, find increasing substrate...
Morphogenesis requires dynamic coordination between cell–cell adhesion and the cytoskeleton to allow cells change shape move without losing tissue integrity. We used genetic tools superresolution microscopy in a simple model epithelial cell line define how molecular architecture of zonula adherens (ZA) is modified response elevated contractility, these maintain previously found that depleting occludens 1 (ZO-1) family proteins MDCK induces highly organized contractile actomyosin array at ZA....
Significance The observation that cells sense and adapt to the physical stiffness of their environment is consistent across many different cell types has profound implications in final biological phenotype. In a diseased state or transformed cell, substrate rigidity sensing adaptation machinery perturbed outright abolished, which leads progression disease, thus fully understanding this process vital. Here, we found organization actin cytoskeleton fibroblasts was governed by active passive...
Pleiomorphic mouse sarcoma S180 cells were transfected with cDNAs for the liver cell adhesion molecule (L-CAM), neural (N-CAM), or both CAMs. Transfected expressed appropriate CAMs at their surface and those expressing L-CAM (S180L cells) changed from adjoining spindle round shapes to a closely linked "epithelioid" sheet when grown confluence. Cells cDNA N-CAM (S180N also this CAM on surfaces bound brain vesicles containing but showed no phenotypic change an epithelioid state. In S180L...
Cell adhesion molecules (CAMs) are cell surface glycoproteins that may play a variety of roles in morphogenesis and histogenesis, particularly defining borders discrete populations. To examine the influence CAM expression on such segregation events vitro, we have transfected cells with cDNAs coding for two calcium-dependent CAMs different specificity, liver (L-CAM) structurally related molecule N-cadherin. The were introduced separately or together into murine sarcoma S180 cells, which...
Breaking the Barrier Being able to deliver drugs into brain treat degenerative diseases such as Alzheimer's or Parkinson's requires ability traverse blood-brain barrier (BBB). Understanding formation of very specific adherent junctions (AJ) and tight present at BBB cell is a prerequisite design therapeutics. However, diminishing expression any one component involved in these intercellular destroys them. Coureuil et al. (p. 83 , published online 11 June) exploited recruitment AJ proteins by...
Mechanical forces play an important role in the organization, growth and function of living tissues. The ability cells to transduce mechanical signals is governed by two types microscale structures: focal adhesions, which link extracellular matrix, adherens junctions, adjacent through cadherins. Although many studies have examined induced there little known about junctions force-regulation processes. present study focuses on determination force transduction cadherins at a single cell level....
Maintaining cell cohesiveness within tissues requires that intercellular adhesions develop sufficient strength to support traction forces applied by myosin motors and neighboring cells. Cadherins are transmembrane receptors mediate adhesion. The cadherin cytoplasmic domain recruits several partners, including catenins vinculin, at sites of cell-cell Our study used force measurements address the role αE-catenin vinculin in regulation E-cadherin-based αE-catenin-deficient cells display only...
The adhesion molecule N-cadherin plays important roles in the development of nervous system, particular by stimulating axon outgrowth, but molecular mechanisms underlying this effect are mostly unknown. One possibility, so-called "molecular clutch" model, could involve a direct mechanical linkage between at membrane and intracellular actin-based motility within neuronal growth cones. Using live imaging primary rat hippocampal neurons plated on N-cadherin-coated substrates optical trapping...
The shaping of a multicellular body and repair adult tissues require fine--tuning cell adhesion, mechanics, intercellular transmission mechanical load. Adherens junctions (AJs) are the major by which cells sense exert force on each other. However, how AJs adapt to stress this adaptation contributes cell-cell cohesion eventually tissue-scale dynamics mechanics remains largely unknown. Here, analyzing tension-dependent recruitment vinculin, α-catenin, F-actin as function stiffness, well...
Living cells actively migrate in their environment to perform key biological functions-from unicellular organisms looking for food single such as fibroblasts, leukocytes or cancer that can shape, patrol invade tissues. Cell migration results from complex intracellular processes enable cell self-propulsion, and has been shown also integrate various chemical physical extracellular signals. While it is established modify by depositing biochemical signals mechanically remodelling the matrix,...