A5012054198- Cellular Mechanics and Interactions
- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- Microtubule and mitosis dynamics
- Bone Tissue Engineering Materials
- Skin and Cellular Biology Research
- Developmental Biology and Gene Regulation
- Neuroscience and Neural Engineering
- Nonlinear Dynamics and Pattern Formation
- Mitochondrial Function and Pathology
- Genetics and Neurodevelopmental Disorders
- 3D Printing in Biomedical Research
- Silk-based biomaterials and applications
- Erythrocyte Function and Pathophysiology
- Force Microscopy Techniques and Applications
- Material Properties and Applications
- Muscle activation and electromyography studies
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Calcium Carbonate Crystallization and Inhibition
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Robotic Locomotion and Control
- Cognitive Science and Education Research
NTT (Japan)
2025
Tokushima University
2020-2025
Keio University Shonan Fujisawa
2020
National University of Singapore
2016-2018
Temasek Life Sciences Laboratory
2016-2017
National Institute for Basic Biology
2009-2017
The Graduate University for Advanced Studies, SOKENDAI
2010-2017
National Institutes of Natural Sciences
2017
Kyoto Medical Center
2017
University of Electro-Communications
2011
Heterogeneity is a critical determinant for multicellular pattern formation. Although the importance of microscale and macroscale heterogeneity at single-cell whole-system levels, respectively, has been well accepted, presence functions mesoscale heterogeneity, such as cell clusters with distinct properties, have poorly recognized. We investigated biological in signal-relaying abilities (excitability) self-organization spiral waves intercellular communications by studying self-organized...
The cell cortex is essential to maintain animal shape, and contractile forces generated within it by nonmuscle myosin II (NMY-2) drive cellular morphogenetic processes such as cytokinesis. role of actin cross-linking proteins in cortical dynamics still incompletely understood. Here, we show that the evolutionarily conserved bundling/cross-linking protein plastin instrumental for generation potent actomyosin contractility
Closure of the neural tube requires both change and maintenance cell shape. The occurs mainly through two coordinated morphogenetic events: elongation apical constriction. How cytoskeletal elements, including microtubules, are regulated in this process vivo is largely unknown. Here, we show that closure Xenopus depends on orthologs proteins: MID1, which responsible for Opitz G/BBB syndrome humans, its paralog MID2. Depletion MIDs (xMIDs) by morpholino-mediated knockdown disrupted epithelial...
Early in the development of central nervous system, progenitor cells undergo a shape change, called apical constriction, that triggers neural plate to form tubular structure. How constriction is controlled and how it contributes tissue morphogenesis are not fully understood. In this study, we show intracellular calcium ions (Ca2+) required for Xenopus tube formation there two types Ca2+-concentration changes, single-cell multicellular wave-like fluctuation, developing plate. Quantitative...
The establishment of cell polarity is crucial for embryonic cells to acquire their proper morphologies and functions, because alignment intracellular events are coordinated in tissues during embryogenesis according the polarity. Although much known about molecules involved polarization, direct trigger process remains largely obscure. We previously demonstrated that tissue boundary between chordamesoderm lateral mesoderm Xenopus laevis important chordamesodermal Here, we examined calcium...
Abstract Genetically encoded Ca 2+ indicators (GECIs) are versatile for live imaging of cellular activities. Besides the brightness and dynamic range signal change GECIs, affinity is another critical parameter successful imaging, as concentration dynamics differs from low nanomolar to sub-millimolar depending on celltype organism. However, ultrahigh-affinity particularly single fluorescent protein (1FP)-type, lacking. Here, we report a simple strategy that increases through linker length...
Summary The spiral wave is a commonly observed spatio-temporal order in diverse signal relaying systems. Although properties of generated spirals have been well studied, the mechanisms for their spontaneous generation living systems remain elusive. By newly developed imaging system trans-scale observation intercellular communication among ∼130,000 cells social amoeba, we investigated onset dynamics cAMP signaling and identified self-organization at three distinct scalings: At...
Abstract Elongated tubular endosomes play essential roles in diverse cellular functions. Multiple molecules have been implicated tubulation of recycling endosomes, but the mechanism endosomal tubule biogenesis has remained unclear. In this study, we found that JRAB/MICAL-L2 induces via activated Rab8A. association with Rab8A, adopts its closed form, which functions endosomes. Moreover, liquid–liquid phase separation, initiating formation upon overexpression. Between N-terminal and C-terminal...
The receptors of gamma-aminobutyric acid (GABA), which is a well-known neurotransmitter, are expressed in the anterior-to-mid neural tube at an early stage Xenopus development, but there has been no report on role GABA presumptive central nervous system. Therefore, we tried to reveal function for embryogenesis. We first confirmed that region expressing gene encoding glutamate decarboxylase 1 (gad1), enzyme catalyzes decarboxylation L-glutamate GABA, overlapped with several genes (gabr) tube....
A 52-year-old woman was admitted because of dysphagia and an oppressive feeling in the chest. Chest X-ray, esophagogram, esophagoscopy, EUS, CT, MRI, bronchoscopy bronchial artery angiography findings led to a diagnosis middle posterior mediastinal tumor (bronchogenic cyst). At surgery, mass originating muscle coat esophagus identified successfully removed along with part esophageal mucosa. The resected specimen solid 7×5×5cm size. Microscopically, composed spindle cells irregular fascicles,...
Establishment ofthe anisotropic