A5101587668- Cellular transport and secretion
- Protein Kinase Regulation and GTPase Signaling
- Microtubule and mitosis dynamics
- Signaling Pathways in Disease
- Cellular Mechanics and Interactions
- Lipid Membrane Structure and Behavior
- Erythrocyte Function and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
- Retinal Development and Disorders
- Pancreatic function and diabetes
- Cell Adhesion Molecules Research
- Barrier Structure and Function Studies
- Wnt/β-catenin signaling in development and cancer
- Vietnamese History and Culture Studies
- Cancer-related Molecular Pathways
- Hippo pathway signaling and YAP/TAZ
- Biosensors and Analytical Detection
- Ion channel regulation and function
- Fungal and yeast genetics research
- Biochemical and Structural Characterization
- Advanced Biosensing Techniques and Applications
- Enzyme Structure and Function
- Silicon Nanostructures and Photoluminescence
- Metabolism, Diabetes, and Cancer
- Neuroscience and Neuropharmacology Research
Tokushima University
2013-2024
ABB (Japan)
2023
AbbVie (Japan)
2023
Rikkyo University
1999-2020
Duke Medical Center
1998-2013
First Hospital of Jilin University
2013
Jilin University
2013
University of Portsmouth
2013
Washington University in St. Louis
2013
College of Law
2011
The ERM proteins, ezrin, radixin, and moesin, are involved in the actin filament/plasma membrane interaction as cross-linkers. CD44 has been identified one of major binding partners for proteins. To examine CD44/ERM protein vitro, we produced mouse glutathione-S-transferase (GST)/CD44 cytoplasmic domain fusion (GST-CD44cyt) by means recombinant baculovirus infection, constructed an vitro assay between proteins CD44. In this system, bound to GST-CD44cyt with high affinity (Kd moesin was 9.3...
The Rho small G protein family, consisting of the Rho, Rac, and Cdc42 subfamilies, regulates various cell functions, such as shape change, motility, cytokinesis, through reorganization actin cytoskeleton. We show here that Rac subfamilies furthermore regulate cell-cell adhesion. prepared MDCK lines stably expressing each dominant active mutants RhoA (sMDCK-RhoDA), Rac1 (sMDCK-RacDA), (sMDCK-Cdc42DA) negative (sMDCK-RacDN) (sMDCK-Cdc42DN) analyzed adhesion in these lines. filaments at sites...
The Rho GDP dissociation inhibitor (GDI) forms a complex with the GDP-bound form of family small G proteins and inhibits their activation. complexed GDI is not activated by GDP/GTP exchange factor for members, suggesting presence another necessary this We have reported that subfamily members regulate ezrin/radixin/moesin (ERM)-CD44 system, implicated in reorganization actin filaments. Here we report directly interacts ERM, initiating activation reducing activity. These results suggest ERM as...
Abstract The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significantly enhancing limit (LOD) is revealed exceptionally large gold nanoparticles (AuNPs) with diameters hundreds nm. SPR enhanced by these lowered LOD N to 85 fM, resulting highest sensitivity ever obtained protein.
Rab proteins comprise a family of small GTPases that serve regulatory role in membrane traffic. These are part cytosolic and associated with the membranes specific exocytic endocytic organelles. Smg p25A/rab3A GDI, protein which inhibits dissociation GDP from smg p25A/rab3A, Sec4p, rab11, has also been found to prevent association rab3A membrane. In this study, we have used Madin-Darby canine kidney cells permeabilized bacterial toxin streptolysin O test general activity GDI modulating...
A novel regulatory protein for smg p25A, a ras p21-like GTP-binding protein, was purified to near homogeneity from bovine brain cytosol. This designated here as p25A GDP dissociation inhibitor (GDI), inhibited the of GDP, but not guanosine 5'-(3-O-thio)triphosphate (GTPgamma S), p25A. GDI also binding GTPgamma S GDP-bound form that guanine nucleotide-free form. did stimulate GTPase activity and by itself showed neither GTPgammaS-binding nor activity. inactive other p21/ras proteins including...
Munc-18/n-Sec1/rbSec1 interacts with syntaxin and this interaction inhibits the association of vesicle-associated membrane protein (VAMP)/synaptobrevin synaptosomal-associated 25 kDa (SNAP-25) syntaxin. Syntaxin, VAMP, SNAP-25 serve as soluble N-ethylmaleimide-sensitive fusion attachment (SNAP) receptors essential for docking and/or synaptic vesicles presynaptic plasma membrane. Genetic analyses in yeast, Caenorhabditis elegans, Drosophila suggest that Munc-18 is vesicle transport. On other...
Evidence is accumulating that rho p21, a ras p21-related small GTP-binding protein (G protein), regulates the actomyosin system. The system known to be essential for cell motility. In present study, we examined action of its inhibitory GDP/GTP exchange (named GDI), stimulatory smg GDS), and Clostridium botulinum ADP-ribosyltransferase C3, selectively ADP-ribosylate p21 impair function, in motility (chemokinesis) Swiss 3T3 cells. We quantitated capacity by measuring tracks phagokinesis....
The endocytic and autophagic pathways are involved in the membrane trafficking of exogenous endogenous materials to lysosomes. However, mechanisms that regulate these largely unknown. We previously reported Rubicon, a Beclin 1–binding protein, negatively regulates both by unidentified mechanisms. In this study, we performed database searches identify potential Rubicon homologues share common C-terminal domain, termed RH domain. One them, PLEKHM1, causative gene osteopetrosis, also suppresses...
The Rab small G protein family, consisting of nearly 30 members, is implicated in intracellular vesicle trafficking. They cycle between the GDP-bound and GTP-bound forms, latter converted to former by action a GTPase activating (GAP). No GAP specific for each family member or subfamily has been isolated mammal. Here we purified with Rab3A as substrate from rat brain. was specifically active on Rab3 members (Rab3A, -B, -C, -D). Of this subfamily, -C are Ca<sup>2+</sup>-dependent exocytosis,...
Insulin and hepatocyte growth factor (HGF) induced morphologically different membrane rufflings in KB cells. Insulin-induced ruffling was inhibited by microinjection of rho GDI, an inhibitory GDP/GTP exchange regulator for both p21 rac small GTP-binding proteins, but not botulinum exoenzyme C3, known to selectively ADP-ribosylate impair its function. This GDI action prevented comicroinjection with guanosine 5'-(3-O-thio)triphosphate (GTP gamma S)-bound rac1 p21. In contrast, HGF-induced or...
rac1 and rac2 p21s are ras p21-like small GTP-binding proteins which implicated in the NADPH oxidase-catalyzed superoxide generation phagocytes. have a Cys-A-A-Leu (A = aliphatic amino acid) structure their C-terminal region may undergo post-translational processing including prenylation, proteolysis, carboxyl methylation. We studied function of this rac interaction with stimulatory inhibitory GDP/GTP exchange for p21s, named smg GDS rho GDI, oxidase activation. produced human recombinant...
During epithelial morphogenesis, adherens junctions (AJs) and tight (TJs) undergo dynamic reorganization, whereas polarity is transiently lost reestablished. Although ARF6-mediated endocytic recycling of E-cadherin has been characterized implicated in the rapid remodeling AJs, molecular basis for rearrangement TJs remains elusive. Occludin claudins are integral membrane proteins comprising TJ strands thought to be responsible establishing maintaining polarity. Here we investigated...
Doc2 has two C2 domains that interact with Ca2+ and phospholipid. Munc13 one C1 domain interacts phorbol ester or diacylglycerol (DAG) Both are implicated in Ca2+-dependent neurotransmitter release, but their modes of action still remain unclear. We show here both a cell-free system intact PC12 cells during the high K+-induced exocytosis. The Doc2-Munc13 interactions stimulated by through Munc13. Overexpression Doc2-interacting reduces exocytosis from cells, co-expression suppresses this...
The smg-21 GTP-binding protein (smg p21) has the same effector domain as ras proteins (ras p21s) and is identical with of rap1A Krev-1 genes. In this paper, two stimulating GTPase activity smg p21 are partially purified from bovine brain cytosol. These proteins, designated GTPase-activating (GAP) 1 2, separated a c-ras GAP described previously by column chromatographies. GAP1 −2 stimulate only but not that c-Ha-ras or rho smg-25A proteins. does dissociation guanosine...
The amyloid precursor protein (APP) is anterogradely transported by conventional kinesin in a distinct transport vesicle, but both the biochemical composition of such vesicle and specific kinesin-1 motor responsible for are poorly defined. APP may be sequentially cleaved β- γ-secretases leading to accumulation β-amyloid (Aβ) peptides brains Alzheimer's disease patients, whereas cleavage α-secretases prevents Aβ generation. Here, we demonstrate time-lapse analysis immunoisolations that cargo...
The assembly of tight junctions (TJs) and adherens (AJs) is regulated by the transport integral TJ AJ proteins to and/or from plasma membrane (PM) it tightly coordinated in epithelial cells. We previously reported that Rab13 a junctional Rab13-binding protein (JRAB)/molecule interacting with CasL-like 2 (MICAL-L2) mediated endocytic recycling an occludin formation functional TJs. Here, we investigated role JRAB/MICAL-L2 other claudin-1 E-cadherin PM using Ca(2+)-switch model. Although...
Abstract Background: Invadopodia are membrane protrusions into the extracellular matrix by aggressive tumour cells. These structures associated with sites of degradation and invasiveness malignant cells in an vitro fibronectin degradation/invasion assay. The Rho family small G proteins, consisting Rho, Rac Cdc42 subfamilies, implicated various cell functions, such as shape change, adhesion, motility, through reorganization actin cytoskeleton. We studied roles proteins invadopodia formation....