A5040178962- Salivary Gland Disorders and Functions
- Biosensors and Analytical Detection
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Nanoparticles: synthesis and applications
- IL-33, ST2, and ILC Pathways
- Advanced Biosensing Techniques and Applications
- SARS-CoV-2 detection and testing
- Salivary Gland Tumors Diagnosis and Treatment
- Graphene and Nanomaterials Applications
- Ubiquitin and proteasome pathways
- Chemokine receptors and signaling
- T-cell and B-cell Immunology
- Inflammasome and immune disorders
- Wnt/β-catenin signaling in development and cancer
- Angiogenesis and VEGF in Cancer
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- interferon and immune responses
- Cancer-related Molecular Pathways
- COVID-19 Clinical Research Studies
- Air Quality and Health Impacts
- Pancreatitis Pathology and Treatment
- Immune Response and Inflammation
- Linguistics and Language Studies
Tokushima University
2016-2025
Abstract The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significantly enhancing limit (LOD) is revealed exceptionally large gold nanoparticles (AuNPs) with diameters hundreds nm. SPR enhanced by these lowered LOD N to 85 fM, resulting highest sensitivity ever obtained protein.
Abstract Signal transmission from the mechanical forces to various intracellular activities is a fundamental process during tissue development. Despite their critical role, mechanism of in biological poorly understood. In this study, we demonstrated that response hydrostatic pressure (HP), piezo type mechanosensitive ion channel component 1 (PIEZO1) primary mechanosensing receptor for odontoblast differentiation through coordination WNT expression and ciliogenesis. stem cells human...
Macrophages (MΦs) are critical regulators of immune response and serve as a link between innate acquired immunity. The precise mechanism involvement tissue-resident MΦs in the pathogenesis autoimmune diseases is not clear. Here, using murine model for Sjögren's syndrome (SS), we investigated role onset development autoimmunity. Two unique populations CD11bhigh CD11blow resident were observed target tissue SS model. Comprehensive gene expression analysis chemokines revealed effective...
FXR is a key molecule that modulates anti-inflammatory activity in the intestinal-liver axis. Although has pleiotropic functions including regulation of liver inflammation and activation macrophages, it remains unclear whether involved macrophage polarization. In this paper we demonstrated stimulation macrophages derived from bone marrow using an agonist activated polarization toward M2 but not M1 macrophages. The treatment mice with chitin skewed towards while co-treatment further promoted...
Abstract SARS‐CoV‐2‐related proteins, ACE2 and TMPRSS2, are determinants of SARS‐CoV‐2 infection. Although these proteins expressed in oral‐related tissues, their expression patterns modulatory mechanisms the salivary glands remain unknown. We herein showed that full‐length ACE2, which has both a fully functional enzyme catalytic site high‐affinity spike S1‐binding sites, was more highly than oral mucosal epithelial cells lungs. Regarding zymogen cleaved form were glands, whereas only murine...
Abstract The sensing of various extrinsic stimuli triggers the receptor-interacting protein kinase-3 (RIPK3)-mediated signaling pathway, which leads to mixed-lineage kinase-like (MLKL) phosphorylation followed by necroptosis. Although necroptosis is a form cell death and involved in inflammatory conditions, roles acute pancreatitis (AP) remain unclear. In current study, we administered caerulein Ripk3- or Mlkl- deficient mice (Ripk3 −/− Mlkl mice, respectively) assessed AP. We found that...
NLRC4 acts as an intracellular sensor to detect bacterial invasion, and activation leads the production of IL-1β IL-18 together with pyroptosis-mediated cell death. Missense active mutations in cause autoinflammatory disorders whose symptoms are distinctly dependent on site mutation other aspects genetic background. To determine involvement inflammation induced by mutation, we depleted IL-1β, or both cytokines Nlrc4-transgenic mice which mutant Nlrc4 is expressed under MHC class II promoter...
Objective Innate lymphoid cells (ILCs), including natural killer (NK) cells, ILC1, ILC2, tissue-inducer (LTi) and ILC3 cell, play a key role in various immune responses. Primary Sjögren’s syndrome (pSS) is an autoimmune disease characterized by chronic inflammation of exocrine glands, such as the lacrimal salivary glands (SGs). The NK among ILCs pathogenesis pSS still unclear. In this study, characteristics subsets gland (SG) tissue were analyzed using murine model pSS. Methods Multiple...
Rapid, sensitive detection of biomolecules is important for biosensing infectious pathogens as well biomarkers and pollutants. For example, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) still strongly required the fight against disease 2019 (COVID-19) pandemic. Here, we aim to achieve rapid SARS-CoV-2 nucleocapsid protein antigen by enhancing performance optical based on frequency combs (OFC). The virus-concentration-dependent spectrum shift produced antigen-antibody...
Nanomaterials are widely used in various fields. Although the toxicity of carbon nanotubes (CNTs) pulmonary tissues has been demonstrated, toxicological effect CNTs on immune system lung remains unclear.In this study, exposure to Taquann-treated multi-walled (T-CNTs) was performed using aerosols generated an inhalation chamber. At 12 months after T-CNT exposure, alveolar inflammation with macrophage accumulation and hypertrophy walls were observed. In addition, fibrotic lesions enhanced by...
We report a near-infrared surface plasmon resonance (SPR) system to achieve highly sensitive, unlabeled detection of the SARS-CoV-2 nucleocapsid protein antigen. Use light in SPR makes dip angular spectrum sharp and causes large change reflected intensity at fixed incident angle. The present achieves resolution 10 −5 refractive index unit measurement glycerol solution samples. Additionally, we measured antigen down molar concentration 1 fM by immobilizing its corresponding antibody on sensor...
Immunoproteasomes regulate the degradation of ubiquitin-coupled proteins and generate peptides that are preferentially presented by MHC class I. Mutations in immunoproteasome subunits lead to dysfunction, which causes proteasome-associated autoinflammatory syndromes (PRAAS) characterized nodular erythema partial lipodystrophy. It remains unclear, however, how dysfunction leads inflammatory symptoms. Here, we established mice harboring a mutation Psmb8 (Psmb8-KI mice) addressed this question....
Abstract Dry mouth is caused by the dysfunction of salivary glands due to various factors. In order understand mechanism dry or develop new therapies for mouth, many mice models have been widely used analyze function saliva secretion. this study, a small amount was collected using oral pH paper, following stimulation with low dose pilocarpine in mice. The method simple and accurate evaluation volumes secretion weak Thus, would be useful during experiments detection saliva. establishment...
Introduction Primary Sjögren’s syndrome (pSS) is a systemic autoimmune disease that affects the function of exocrine glands, such as lacrimal and salivary glands. Extraglandular lesions malignant lymphoma also occur during progressive stage pSS. We have, herein, focused on pulmonary pSS have aimed clarifying their pathophysiological mechanism by comparing glandular with extraglandular observed in mouse model Results The histopathological analysis lung tissues obtained from NFS/ sld mice...
We demonstrated rapid detection of SARS-CoV-2 nucleocapsid protein antigen by dual-comb biosensing with surface modification its corresponding antibody. A sensitivity close to that RT-PCR was achieved, thanks the use active–dummy temperature compensation.
Abstract Rapid, sensitive detection of biomolecules is important for improved testing methods infectious pathogens as well biomarkers and pollutants. For example, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) essential in the fight against disease 2019 (COVID-19) pandemic. Reverse-transcription polymerase chain reaction (RT-PCR) current standard COVID-19 testing; however, it hampered by long process. Simplifying shortening process while achieving its high sensitivity would...
Abstract Background The toxicological effects of nanomaterials, such as multi-walled carbon nanotubes (MWCNTs), on the immune system are considerably understood. However, precise relationship between long-term exposure to MWCNTs and chronic inflammation remains unclear. Methods Finding In this study, Taquann-treated (T-CNTs) was performed using aerosols generated in an inhalation chamber. At 12 months after T-CNT exposure, alveolar with macrophage accumulation hypertrophy walls were...