A5052499296- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Biosensors and Analytical Detection
- Microtubule and mitosis dynamics
- Advanced Biosensing Techniques and Applications
- Hippo pathway signaling and YAP/TAZ
- Pancreatic function and diabetes
- SARS-CoV-2 detection and testing
- Erythrocyte Function and Pathophysiology
- Advanced biosensing and bioanalysis techniques
- Retinal Development and Disorders
- Genetics and Neurodevelopmental Disorders
- SARS-CoV-2 and COVID-19 Research
- Cell Adhesion Molecules Research
- Tendon Structure and Treatment
- Wound Healing and Treatments
- Wnt/β-catenin signaling in development and cancer
- Hedgehog Signaling Pathway Studies
- Mechanical and Optical Resonators
- Fullerene Chemistry and Applications
- Alzheimer's disease research and treatments
- Genetic and Kidney Cyst Diseases
- Autophagy in Disease and Therapy
- Calcium signaling and nucleotide metabolism
- Renal Diseases and Glomerulopathies
Tokushima University
2015-2024
Photonics (United States)
2021
Tokyo Medical and Dental University
2012
China Medical University
2012
Juntendo University
2012
Abstract The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significantly enhancing limit (LOD) is revealed exceptionally large gold nanoparticles (AuNPs) with diameters hundreds nm. SPR enhanced by these lowered LOD N to 85 fM, resulting highest sensitivity ever obtained protein.
The endocytic and autophagic pathways are involved in the membrane trafficking of exogenous endogenous materials to lysosomes. However, mechanisms that regulate these largely unknown. We previously reported Rubicon, a Beclin 1–binding protein, negatively regulates both by unidentified mechanisms. In this study, we performed database searches identify potential Rubicon homologues share common C-terminal domain, termed RH domain. One them, PLEKHM1, causative gene osteopetrosis, also suppresses...
The amyloid precursor protein (APP) is anterogradely transported by conventional kinesin in a distinct transport vesicle, but both the biochemical composition of such vesicle and specific kinesin-1 motor responsible for are poorly defined. APP may be sequentially cleaved β- γ-secretases leading to accumulation β-amyloid (Aβ) peptides brains Alzheimer's disease patients, whereas cleavage α-secretases prevents Aβ generation. Here, we demonstrate time-lapse analysis immunoisolations that cargo...
Rab3A small G protein is a member of the Rab family and most abundant in brain, where it localized on synaptic vesicles. Evidence accumulating that plays key role neurotransmitter release plasticity. cycles between GDP-bound inactive GTP-bound active forms, this change activity associated with trafficking cycle vesicles at nerve terminals. Rab3 GTPase-activating (GAP) stimulates GTPase expected to determine timing dissociation from vesicles, which may be coupled vesicle exocytosis. GAP...
Neurite outgrowth is the first step in processes of neuronal differentiation and regeneration leads to synaptic polarization plasticity. Rab13 small G protein shows an increased mRNA expression level during regeneration; it therefore thought be involved this process. We previously identified JRAB (junctional Rab13-binding protein)/MICAL-L2 (molecules interacting with CasL-like 2) as a novel effector protein. Here, we show that regulates neurite rat pheochromocytoma cell line PC12 through...
Actomyosin-undercoated adherens junctions are critical for epithelial cell integrity and remodeling. Actomyosin associates with through αE-catenin complexed β-catenin E-cadherin in vivo; however, vitro biochemical studies solution showed that binds to F-actin less efficiently than is not β-catenin. Although a “catch-bond model” partly explains this inconsistency, the mechanism inconsistency between vivo results remains elusive. We herein demonstrate afadin enhances its F-actin–binding...
The Doc2 family comprises the brain-specific Doc2alpha and ubiquitous Doc2beta Doc2gamma. With exception of Doc2gamma, these proteins exhibit Ca(2+)-dependent phospholipid-binding activity in their Ca(2+)-binding C2A domain are thought to be important for regulated exocytosis. In excitatory neurons, interacts with Munc13-1, a member Munc13 family, through its N-terminal Munc13-1-interacting Doc2alpha-Munc13-1 system is implicated synaptic vesicle Munc13-2, Munc13-3, non-neuronal Munc13-4. We...
In fundamental biological processes, cells often move in groups, a process termed collective cell migration. Collectively migrating are much better organized than random assemblage of individual cells. Many molecules have been identified as factors involved migration, and no one molecule is adequate to explain the whole picture. Here we show that JRAB/MICAL-L2, an effector protein Rab13 GTPase, provides “law order” allowing myriad behave single unit just by changing its conformation. First,...
Journal Article Expression of RASSF6 in kidney and the implication Hippo pathway sorbitol-induced apoptosis renal proximal tubular epithelial cells Get access Kanchanamala Withanage, Withanage 1Department Medical Biochemistry, Graduate School Medicine, Tokyo Dental University, 113-8519; 2Department Anatomy, Juntendo University 113-8421; 3Department Neurosurgery, 113-8519, Japan; 4Department Ultrasound, Shengjing Hospital China Shenyang 110004, China; 5Department Institute Health Biosciences,...
Dynamin 1 is a neuronal endocytic protein that participates in vesicle formation by scission of invaginated membranes. also expressed the kidney; however, its physiological significance to this organ remains unknown. Here, we show dynamin crucial for microtubule organization and stabilization glomerular podocytes. By immunofluorescence immunoelectron microscopy, was concentrated at microtubules primary processes rat differentiated mouse podocytes (MPCs), often colocalized with bundles, which...
We previously showed that R ab13 and its effector protein, junctional ab13‐binding protein ( JRAB )/molecules interacting with C as L ‐like 2 MICAL ‐L2), regulate development by modulating cell adhesion molecule transport actin cytoskeletal reorganization in epithelial cells. Here, we investigated how regulates of the cytoskeleton NIH 3T3 fibroblasts, an attempt to obtain novel insights into mechanism action. To this end, expressed mutant proteins adopt a constitutively open or closed state...
Rapid, sensitive detection of biomolecules is important for biosensing infectious pathogens as well biomarkers and pollutants. For example, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) still strongly required the fight against disease 2019 (COVID-19) pandemic. Here, we aim to achieve rapid SARS-CoV-2 nucleocapsid protein antigen by enhancing performance optical based on frequency combs (OFC). The virus-concentration-dependent spectrum shift produced antigen-antibody...
Abstract JRAB/MICAL-L2 is an effector protein of Rab13, a member the Rab family small GTPase. consists calponin homology domain, LIM and coiled-coil domain. engages in intramolecular interaction between N-terminal domain C-terminal changes its conformation from closed to open under effect Rab13. Open-form induces formation peripheral ruffles via filamin. Here, we report that independent C-terminus, also necessary for function open-form JRAB/MICAL-L2. In mechanistic terms, two zinc finger...
Abstract Genetically encoded Ca 2+ indicators (GECIs) are versatile for live imaging of cellular activities. Besides the brightness and dynamic range signal change GECIs, affinity is another critical parameter successful imaging, as concentration dynamics differs from low nanomolar to sub-millimolar depending on celltype organism. However, ultrahigh-affinity particularly single fluorescent protein (1FP)-type, lacking. Here, we report a simple strategy that increases through linker length...
Double C2 domain protein b (DOC2b), one of the synaptotagmins, has been shown to translocate plasma membrane, and initiate membrane-fusion processes vesicles containing glucose transporter 4 proteins on insulin stimulation. However, mechanism by which DOC2b is regulated remains unclear. Herein, we identified upstream regulatory factors in signal transduction. We also examined role systemic homeostasis using knockout (KO) mice.We first binding immunoprecipitation mutagenesis experiments....
A hippocampal mossy fiber synapse implicated in learning and memory is a complex structure which presynaptic bouton attaches to the dendritic trunk by puncta adherentia junctions (PAJs) wraps multiply branched spines. The postsynaptic densities (PSDs) are localized at heads of each these spines faces active zones. We previously showed that scaffolding protein afadin regulates formation PAJs, PSDs, zones synapse. Afadin has two splice variants: l-afadin s-afadin. l-Afadin, but not s-afadin,...
Abstract Elongated tubular endosomes play essential roles in diverse cellular functions. Multiple molecules have been implicated tubulation of recycling endosomes, but the mechanism endosomal tubule biogenesis has remained unclear. In this study, we found that JRAB/MICAL-L2 induces via activated Rab8A. association with Rab8A, adopts its closed form, which functions endosomes. Moreover, liquid–liquid phase separation, initiating formation upon overexpression. Between N-terminal and C-terminal...
We report a near-infrared surface plasmon resonance (SPR) system to achieve highly sensitive, unlabeled detection of the SARS-CoV-2 nucleocapsid protein antigen. Use light in SPR makes dip angular spectrum sharp and causes large change reflected intensity at fixed incident angle. The present achieves resolution 10 −5 refractive index unit measurement glycerol solution samples. Additionally, we measured antigen down molar concentration 1 fM by immobilizing its corresponding antibody on sensor...
The apical junctional complex (AJC) consists of adherens junctions (AJs) and tight regulates epithelial integrity remodeling. However, it is unclear how AJC organization regulated based on environmental cues. We found here using cultured EpH4 mouse mammary cells that fetal bovine serum (FBS) in a culture medium showed an activity to promote FBS junction formation even the absence AJ proteins, such as E-cadherin, αE-catenin, afadin. Furthermore, we purified individual factor responsible for...
We demonstrated rapid detection of SARS-CoV-2 nucleocapsid protein antigen by dual-comb biosensing with surface modification its corresponding antibody. A sensitivity close to that RT-PCR was achieved, thanks the use active–dummy temperature compensation.