A5013105441- Inflammatory Bowel Disease
- Gut microbiota and health
- Wnt/β-catenin signaling in development and cancer
- Microscopic Colitis
- Cellular transport and secretion
- Helicobacter pylori-related gastroenterology studies
- Gastric Cancer Management and Outcomes
- Colorectal Cancer Screening and Detection
- Protein Kinase Regulation and GTPase Signaling
- Retinal Development and Disorders
- Skin and Cellular Biology Research
- Diet and metabolism studies
- Adolescent and Pediatric Healthcare
- RNA and protein synthesis mechanisms
- Liver Disease Diagnosis and Treatment
- Immune Cell Function and Interaction
- Clostridium difficile and Clostridium perfringens research
- Neuroscience and Neuropharmacology Research
- Microtubule and mitosis dynamics
- Gastrointestinal motility and disorders
- Immunotherapy and Immune Responses
- Eosinophilic Esophagitis
- Pregnancy and Medication Impact
- Dietary Effects on Health
- Circadian rhythm and melatonin
Osaka Medical Center for Cancer and Cardiovascular Diseases
2008-2025
Kyorin University
2019-2025
Takeda (Japan)
2024
Pfizer (United Kingdom)
2024
Mitsubishi Tanabe Pharma Corporation
2024
Kissei Pharmaceutical (Japan)
2024
EA Pharma (Japan)
2024
Nippon Kayaku (Japan)
2024
Mochida Pharmaceutical (Japan)
2024
Zeria Pharmaceutical (Japan)
2024
Afadin is an actin filament-binding protein that binds to nectin, immunoglobulin-like cell adhesion molecule, and colocalized with nectin at cadherin-based cell-cell adherens junctions (AJs). To explore the function of afadin in during embryogenesis, we generated afadin(-/-) mice embryonic stem cells. In wild-type days 6.5-8.5, was highly expressed ectoderm mesoderm, but hardly detected extraembryonic regions such as visceral endoderm. Afadin(-/-) showed developmental defects stages after...
Hypertension is a leading contributor to cardiovascular mortality worldwide. Despite this, its underlying mechanism(s) and the role of excess salt in cardiorenal dysfunction are unclear. Previously, we have identified cross-talk between mineralocorticoid receptor (MR), nuclear transcription factor regulated by steroid aldosterone, small GTPase Rac1, which implicated proteinuric kidney disease. We here show that high-salt loading activates Rac1 kidneys rodent models salt-sensitive...
Myeloid dendritic cells (mDCs) recognize and respond to polyI:C, an analog of dsRNA, by endosomal Toll-like receptor (TLR) 3 cytoplasmic receptors. Natural killer (NK) are activated in vivo the administration polyI:C mice reciprocally mDCs, although molecular mechanisms as yet undetermined. Here, we show that TLR adaptor TICAM-1 (TRIF) participates mDC-derived antitumor NK activation. In a syngeneic mouse tumor implant model (C57BL/6 vs. B16 melanoma with low H-2 expresser), i.p. led...
In the auditory epithelium of cochlea, sensory hair cells and supporting are arranged in a checkerboard-like fashion, but mechanism underlying this cellular patterning is unclear. We found that mouse express immunoglobulin-like adhesion molecules nectin-1 -3, respectively, their interaction mediates heterotypic between these two cell types. Genetic removal or -3 disrupted pattern, inducing aberrant attachment cells. When expressing either were cocultured, they themselves into mosaic pattern....
Factors affecting the developing neonatal gut microbiome and immune networks may increase risk of complex disorders such as inflammatory bowel diseases (IBD). In particular, peripartum antibiotics have been suggested factors for human IBD, although direct evidence is lacking. Therefore, we examined temporal impact commonly used antibiotic cefoperazone on both maternal offspring microbiota when administered to dams during period in IL-10-deficient murine colitis model. By rigorously...
A new transforming gene has been molecularly cloned from hamster SHOK cells transformed with DNA extracted a human thyroid carcinoma cell line and named the cot (cancer Osaka thyroid) oncogene. cDNA sequencing disclosed that this oncogene codes for protein 415 amino acid residues, computer matching showed 42 to 48% similarity matches serine kinases. Its product was identified as 52-kDa by transcription translation in vitro. Expression of under transcriptional control retroviral long terminal...
<h3>Background</h3> Chronic inflammation characterised by IgG-producing plasma cell infiltration of colonic mucosa is a histological hallmark ulcerative colitis (UC); however, whether its function pathogenic or protective remains unclear. <h3>Objective</h3> To explore the contribution intestinal IgG cells to UC pathogenesis. <h3>Methods</h3> We isolated lamina propria mononuclear (LPMCs) from patients and analysed characteristics (expression profiles differentiation molecules chemokine...
How size and shape of presynaptic active zones are regulated at the molecular level has remained elusive. Here we provide insight from studying rod photoreceptor ribbon-type after disruption CAST/ERC2, one cytomatrix zone (CAZ) proteins. Rod photoreceptors were present in normal numbers, a-wave electroretinogram (ERG)--reflecting their physiological population response--was unchanged CAST knock-out (CAST(-/-)) mice. Using immunofluorescence electron microscopy, found that zones, Ca(2+)...
Osteoclasts are multinucleated giant cells that reside in osseous tissues and resorb bone. Signaling mediated by receptor activator of nuclear factor (NF)-κB (RANK) its ligand leads to the activated T 2/c1 (NFAT2 or NFATc1) expression, a critical step formation functional osteoclasts. In addition, adaptor proteins harboring immunoreceptor tyrosine-based activation motifs, such as DNAX-activating protein 12 kDa (DAP12), play essential roles. this study, we identified gene encoding lectin...
Genotoxic stress and RAS induce the expression of CD155, a ligand for immune receptors DNAM-1, CD96 TIGIT. Here we show that antigen-presenting cells upregulate CD155 in response to Toll-like receptor activation. Induction by depended on MYD88, TRIF NF-κB. In addition, IRF3, but not IRF7, modulated upregulation TLR3 signals. Immunization CD155-deficient mice with OVA TLR9 agonist CpG resulted increased OVA-specific IgG2a/c titers when compared wild type mice. Splenocytes immunized secreted...
Murine models are widely used to explore host-microbe interactions because of the challenges and limitations inherent human studies. However, microbiome studies in murine not without their nuances. Inter-individual variations gut microbiota frequent even animals housed within same room. We therefore sought find an efficient effective standard operating procedure (SOP) minimize these effects improve consistency reproducibility Mice were a single room under specific-pathogen free conditions....
Circadian rhythms govern glucose homeostasis, and their dysregulation leads to complex metabolic diseases. Gut microbes exhibit diurnal that influence host circadian networks processes, yet underlying mechanisms remain elusive. Here, we showed hierarchical, bidirectional communication among the liver clock, gut microbes, homeostasis in mice. To assess this relationship, utilized mice with liver-specific deletion of core clock gene Bmal1 via Albumin-cre maintained either conventional or...
Nectins are Ca2+-independent immunoglobulin-like cell-cell-adhesion molecules consisting of four members. homophilically and heterophilically trans-interact to form a variety cell-cell junctions, including cadherin-based adherens junctions in epithelial cells fibroblasts culture, synaptic neurons, Sertoli cell-spermatid the testis, cooperation with, or independently of, cadherins. To further explore function nectins, we generated nectin 1-/- 3-/-)mice. Both 3-/- mice showed virtually...
Neurites recognize their specific partners during the formation of interneuronal connections. In hippocampal pyramidal neurons, axons attach to dendrites for synaptogenesis, but do not form stable contacts with each other, suggesting presence a mechanism allow selective associations. Nectin-1 (N1), an immunoglobulin domain adhesive protein, is preferentially localized in axons, and its heterophilic partner, N3, present both dendrites; we tested potential roles interneurite recognition. The...