A5020689274- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Respiratory viral infections research
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- Parvovirus B19 Infection Studies
- Viral gastroenteritis research and epidemiology
- Eosinophilic Esophagitis
- Fatty Acid Research and Health
- interferon and immune responses
- T-cell and B-cell Immunology
- Reproductive Biology and Fertility
- NF-κB Signaling Pathways
- SARS-CoV-2 and COVID-19 Research
- Nitric Oxide and Endothelin Effects
- Ovarian function and disorders
- Viral Infections and Immunology Research
- Eicosanoids and Hypertension Pharmacology
- Virology and Viral Diseases
- Viral-associated cancers and disorders
- Dental materials and restorations
- Antimicrobial Resistance in Staphylococcus
- Galectins and Cancer Biology
- RNA and protein synthesis mechanisms
- Neuroscience and Neuropharmacology Research
Akita University
2019-2024
RIKEN Center for Integrative Medical Sciences
2018-2024
National Center for Global Health and Medicine
2020
Washington University in St. Louis
2019
Nippon Dental University
2009-2019
Howard Hughes Medical Institute
2013-2017
Hokkaido University
2005-2016
Tokyo Medical Center
2009-2013
National Hospital Organization
2009-2013
Tokyo Institute of Technology
1999-2007
Epstein-Barr virus–encoded small RNA (EBER) is nonpolyadenylated, noncoding that forms stem-loop structure by intermolecular base-pairing, giving rise to double-stranded (dsRNA)–like molecules, and exists abundantly in EBV-infected cells. Here, we report EBER induces signaling from the Toll-like receptor 3 (TLR3), which a sensor of viral (dsRNA) type I IFN proinflammatory cytokines. A substantial amount EBER, was sufficient induce TLR3, released cells, majority existed as complex with...
ABSTRACT Human bocavirus (HBoV), a newly cloned human virus of the genus Bocavirus , was detected by PCR from nasopharyngeal swab samples (8 318; 5.7%) collected children with lower respiratory tract infections. HBoV may be one causative agents infections in young children.
Myeloid dendritic cells (mDCs) recognize and respond to polyI:C, an analog of dsRNA, by endosomal Toll-like receptor (TLR) 3 cytoplasmic receptors. Natural killer (NK) are activated in vivo the administration polyI:C mice reciprocally mDCs, although molecular mechanisms as yet undetermined. Here, we show that TLR adaptor TICAM-1 (TRIF) participates mDC-derived antitumor NK activation. In a syngeneic mouse tumor implant model (C57BL/6 vs. B16 melanoma with low H-2 expresser), i.p. led...
ABSTRACT Human metapneumovirus (hMPV) has been recently discovered as an etiological agent of acute respiratory infections. Our purpose was to asses the virological and clinical features children with infections caused by hMPV. We examined 658 nasopharyngeal swab samples obtained from 637 for hMPV using reverse transcription-PCR (RT-PCR). A total 268 were inoculated onto tertiary monkey kidney cells. 36 serum (26 in phase 10 convalescent phase) 26 hMPV-positive tested immunoglobulin G (IgG)...
ABSTRACT A new human virus, provisionally named bocavirus (HBoV), was discovered by Swedish researchers in 2005. immunofluorescence assay using Trichoplusia ni insect cells infected with a recombinant baculovirus expressing the VP1 protein of HBoV developed, and levels immunoglobulin G antibody to serum samples were measured. The overall seroprevalence rate antibodies against Japanese population aged from 0 months 41 years 71.1% (145 204). seropositive lowest age group 6 8 gradually...
A new human pneumovirus, provisionally designated metapneumovirus, was discovered by Dutch researchers. We examined 142 serum samples from the general population aged 1 month to 35 years in Japan for metapneumovirus antibody indirect immunofluorescence assays using metapneumovirus-infected monkey kidney cells. The overall prevalence of infection 72.5%. seropositive rate lowest age group 6 months year and gradually increased with age. All children had been exposed 10 years. results show that...
Laboratory adapted and vaccine strains of measles virus (MV) induced type I IFN in infected cells. The wild-type contrast it to a far lesser extent. We have investigated the mechanism for this differential induction monocyte-derived dendritic cells with representative MV strains. Nagahata Edmonston activated IRF-3 followed by IFN-beta production, while MS failed activate IRF-3. viral activation is within 2 h, an early response occurring before protein synthesis. Receptor usage CD46 or CD150...
HCoV-NL63, the fourth human coronavirus, has been isolated recently from children with respiratory tract infections, including upper infection, bronchiolitis, and pneumonia. The virus also detected in immunocompromised adults infections. A total of 118 nasopharyngeal swab samples hospitalized young aged less than 2 years bronchiolitis who were not infected syncytial virus, influenza or B, metaneumovirus selected. Three (2.5%) positive for HCoV-NL63 by reverse transcription-polymerase chain...
In myeloid dendritic cells (mDCs), TLR3 is expressed in the endosomal membrane and interacts with adaptor toll/interleukin 1 receptor homology domain-containing molecule (TICAM-1; TRIF). TICAM-1 signals culminate interferon (IFN) regulatory factor (IRF) 3 activation. Co-culture of mDC pretreated ligand polyI:C natural killer (NK) resulted NK cell This activation was triggered by cell-to-cell contact but not cytokines. Using expression profiling gain/loss-of-function analyses genes, we tried...
Abstract Group 2 innate lymphoid cells (ILC2s) have tissue-resident competence and contribute to the pathogenesis of allergic diseases. However, mechanisms regulating prolonged ILC2-mediated T H cytokine production under chronic inflammatory conditions are unclear. Here we show that, at homeostasis, Runx deficiency induces excessive ILC2 activation due overly active GATA-3 functions. By contrast, during inflammation, absence impairs ability ILC2s proliferate produce effector cytokines...
Dendritic cell maturation critically modulates antiviral immune responses, and facilitates viral clearance. Hepatitis C virus (HCV) is characterized by its high predisposition to persistent infection. Here, we examined the response of human monocyte-derived dendritic cells (MoDCs) JFH1 strain HCV, which can efficiently replicate in culture. However, neither HCV RNA replication nor antigen production was detected MoDCs inoculated with JFH1. None indicators interacting evaluated were affected,...
Monocyte-derived dendritic cells (mDCs) and NK are reciprocally activate via cytokines cell-cell contact. Although seven human NKG2D ligands (NKG2DLs), UL16-binding proteins (ULBP) 1, 2, 3 4, retinoic acid early transcript 1G (RAET1G) MHC class I-related chains A B, have been reported, the differential distribution roles of these in maturation mDCs not elucidated. In present study, we produced polyclonal antibodies (pAbs) directed against ULBP1, 2 3. All ULBPs were detected on when probed by...
Natural killer (NK) cells are lymphocyte effectors that activated to control certain microbial infections and tumors. Many NK-activating regulating receptors involved in NK cell function. In addition, activation of naïve is fundamentally triggered by cytokines or myeloid dendritic (mDC) various modes. this study, we synthesized 16 S-[2,3-bis(palmitoyl)propyl]cysteine (Pam2Cys) lipopeptides with sequences designed from lipoproteins Staphylococcus aureus, assessed their functional properties...
PolyI:C is a nucleotide pattern molecule that induces cross-presentation of foreign Ag in myeloid dendritic cells (DC) and MHC Class I-dependent proliferation cytotoxic T lymphocytes (CTL). DC (BM or spleen CD8α(+)) have sensors for dsRNA including polyI:C to signal facilitating cross-presentation. Endosomal TLR3 cytoplasmic RIG-I/MDA5 are reportedly responsible sensing presumed deliver via TICAM-1 (TRIF) IPS-1 (MAVS, Cardif, VISA) adaptors, respectively. In fact, when tumor-associated (TAA)...
ABSTRACT Complete genes encoding the predicted nucleoprotein (N), phosphoprotein (P), matrix protein (M), fusion (F), M2-1protein, M2-2protein, small hydrophobic (SH), and attachmentprotein (G) of seven newly isolated human metapneumoviruses (hMPVs) were analyzed compared with previously published data for hMPV genes. Phylogenetic analysis nucleotide sequences indicated that there two genetic groups, tentatively named groups 1 2, similar to grouping respiratory syncytial virus. Although...
Monocyte-derived dendritic cells (mDCs) recognize viral RNA extrinsically by Toll-like receptor (TLR) 3 on the membrane and intrinsically retinoic acid-inducible gene I (RIG-I)/melanoma differentiation-associated 5 (MDA5) in cytoplasm to induce type IFNs mDC maturation. When mDCs were treated with live or UV-irradiated respiratory syncytial virus (RSV), early (∼4 h) induction of IFN-β usually occurs other infections was barely observed. Live RSV subsequently replicated activate cytoplasmic...
ABSTRACT Human metapneumovirus (hMPV) is a recently discovered pathogen associated with respiratory tract infections, primarily in young children, immunocompromised individuals, and elderly individuals. Reverse transcription-PCR (RT-PCR) has been reported to be more sensitive method for the diagnosis of hMPV infections than virus isolation by culture serological study. However, there no report on rapid methods, such as an immunofluorescent-antibody test or enzyme-linked immunosorbent assay,...
Oral administration of hot-water extract Spirulina, cyanobacterium Spirulina platensis, leads to augmentation NK cytotoxicity in humans. Here, we applied syngeneic tumor-implant mice (C57BL/6 versus B16 melanoma) elucidate the mechanism raising antitumor activation. A B16D8 subcell line barely expressed MHC class I but about 50% Rae-1, a ligand for activation receptor NKG2D. The Rae-1-positive population implant melanoma was effectively eliminated tumor mass progressed mice. This activity...
Successful cancer immunotherapy necessitates T cell proliferation and infiltration into tumor without exhaustion, a process closely links optimal maturation of dendritic cells (DC), adjuvant promotes this as an essential prerequisite. Poly(I:C) has contributed to that evokes antitumor response through the Toll-loke receptor 3 (TLR3)/TICAM-1 pathway in DC. However, mechanism whereby acts on DC for migration remains undetermined. Subcutaneous injection regressed implant tumors (WT1-C1498 or...