A5102000136- Autophagy in Disease and Therapy
- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Herpesvirus Infections and Treatments
- interferon and immune responses
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Endoplasmic Reticulum Stress and Disease
- MicroRNA in disease regulation
- Immune Response and Inflammation
- COVID-19 Clinical Research Studies
- Advanced Radiotherapy Techniques
- Cancer, Hypoxia, and Metabolism
- Hepatitis B Virus Studies
- RNA Interference and Gene Delivery
- Hepatitis C virus research
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- Circular RNAs in diseases
- Alzheimer's disease research and treatments
- Galectins and Cancer Biology
- Influenza Virus Research Studies
- HIV-related health complications and treatments
Wuhan University
2016-2025
Huazhong Agricultural University
2021-2025
Ministry of Agriculture and Rural Affairs
2025
South China Agricultural University
2025
Sichuan University
2014-2024
New England College of Optometry
2024
Nanjing Normal University
2024
Wuhan Institute of Virology
2021-2024
West China Hospital of Sichuan University
2014-2024
Kunming Medical University
2024
Epstein-Barr virus–encoded small RNA (EBER) is nonpolyadenylated, noncoding that forms stem-loop structure by intermolecular base-pairing, giving rise to double-stranded (dsRNA)–like molecules, and exists abundantly in EBV-infected cells. Here, we report EBER induces signaling from the Toll-like receptor 3 (TLR3), which a sensor of viral (dsRNA) type I IFN proinflammatory cytokines. A substantial amount EBER, was sufficient induce TLR3, released cells, majority existed as complex with...
Abstract Glucose metabolism and innate immunity evolved side-by-side. It is unclear if how the two systems interact with each other during hepatitis B virus (HBV) infections and, so, which mechanisms are involved. Here, we report that HBV activates glycolysis to impede retinoic acid-inducible gene I (RIG-I)-induced interferon production. We demonstrate sequesters MAVS from RIG-I by forming a ternary complex including hexokinase (HK). Using series of pharmacological genetic approaches,...
Abstract Background Hypoxia, a typical hallmark of solid tumors, exhibits an essential role in the progression colorectal cancer (CRC), which dysregulation long non-coding RNAs (lncRNAs) is frequently observed. However, underlying mechanisms are not clearly defined. Methods The TCGA database was analyzed to identify differential lncRNA expression involved hypoxia-induced CRC progression. qRT-PCR conducted validate upregulation STEAP3-AS1 cell lines and tumor-bearing mouse zebrafish models...
Article13 January 2022Open Access Source DataTransparent process Inhibition of NPC1L1 disrupts adaptive responses drug-tolerant persister cells to chemotherapy Zhe Zhang orcid.org/0000-0001-7509-6965 Laboratory Aging Research and Cancer Drug Target, State Key Biotherapy Center, National Clinical Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, School Basic Medical Sciences & Forensic Medicine, Collaborative Innovation Biotherapy, Contribution: Conceptualization, Data...
Advanced mRNA vaccines play vital roles against SARS-CoV-2. However, most current delivery platforms need to be stored at -20 °C or -70 due their poor stability, which severely restricts availability. Herein, we develop a lyophilization technique prepare SARS-CoV-2 mRNA-lipid nanoparticle with long-term thermostability. The physiochemical properties and bioactivities of lyophilized showed no change 25 over 6 months, the could elicit potent humoral cellular immunity whether in mice, rabbits,...
To capitalize on the response of tumor cells to XRT, we developed a controlled-release nanoparticle drug delivery system using targeting peptide that recognizes radiation-induced cell surface receptor. Phage display biopanning identified Gly-Ile-Arg-Leu-Arg-Gly (GIRLRG) as selectively tumors responding XRT. Membrane protein extracts irradiated glioma glucose-regulated GRP78 receptor target for GIRLRG. Antibodies blocked binding GIRLRG in vitro and vivo. Conjugation sustained-release yielded...
It is well known that high-risk human papilloma virus (HR-HPV) infection strongly associated with cervical cancer and E7 was identified as one of the key initiators in HPV-mediated carcinogenesis. Here we show lactate dehydrogenase A (LDHA) preferably locates nucleus HPV16-positive tumors due to E7-induced intracellular reactive oxygen species (ROS) accumulation. Surprisingly, nuclear LDHA gains a non-canonical enzyme activity produce α-hydroxybutyrate triggers DOT1L (disruptor telomeric...
GBM (glioblastoma multiforme) is the most common and aggressive brain tumor with no curative options available. Therefore, it imperative to develop novel potent therapeutic drugs for treatment. Here, we show that regorafenib, an oral multi-kinase inhibitor, exhibits superior efficacy over temozolomide, first-line chemotherapeutic agent treatment both in vitro vivo. Mechanistically, regorafenib directly stabilizes PSAT1 (phosphoserine aminotransferase 1), a critical enzyme serine synthesis,...
In this study, we demonstrated an essential function of the hexosamine biosynthesis pathway (HBP)-associated O-linked β-N-acetylglucosamine (O-GlcNAc) signaling in influenza A virus (IAV)-induced cytokine storm. O-GlcNAc transferase (OGT), a key enzyme for protein O-GlcNAcylation, mediated IAV-induced production. Upon investigating mechanisms driving event, determined that IAV induced OGT to bind interferon regulatory factor-5 (IRF5), leading O-GlcNAcylation IRF5 on serine-430. is required...
Background and Aims Tumor metastasis is a major factor of high recurrence mortality in hepatocellular carcinoma (HCC), but its underlying mechanism remains elusive. We report that PDZ LIM domain protein 1 (PDLIM1) significantly down‐regulated metastatic human HCC tissues, which predicts unfavorable prognosis, suggesting PDLIM1 may play an important inhibitory role during metastasis. Approach Results Functional studies indicate knockdown induces epithelial‐to‐mesenchymal transition (EMT)...
Rationale: Colorectal cancer (CRC) is one of the most common cancers worldwide. Ciclopirox olamine (CPX) has recently been identified to be a promising anticancer candidate; however, novel activities and detailed mechanisms remain uncovered. Methods: The cytotoxic potential CPX towards CRC cells was examined in vitro vivo. global gene expression pattern, ROS levels, mitochondrial function, autophagy, apoptosis, etc. were determined between control CPX-treated cells. Results: We found that...
Pancreatic cancer is one of the most aggressive tumors associated with a poor clinical prognosis, weakly effective therapeutic options. Therefore, there strong impetus to discover new targets in pancreatic cancer. In present study, we first demonstrated that TSPAN1 upregulated and depletion decreases cell proliferation vitro vivo. expression was correlated overall survival patients. Moreover, novel positive regulator macroautophagy/autophagy characterized by decreased LC3-II SQSTM1/p62...
Durable glioblastoma multiforme (GBM) management requires long-term chemotherapy after surgery to eliminate remaining cancerous tissues. Among chemotherapeutics, temozolomide is considered as the first-line drug for GBM therapy, but treatment outcome not satisfactory. Notably, regorafenib, an oral multi-kinase inhibitor, has been reported exert a markedly superior effect on suppression compared with temozolomide. However, poor site-specific delivery and bioavailability significantly restrict...
Global concern has been raised by the emergence and rapid transmission of heavily mutated SARS-CoV-2 Omicron variant (B.1.1.529). So far, infection features immune escape ability have not extensively studied. Here, we produced pseudovirus compared its entry, membrane fusion, efficiency with original strain dominating Delta variant. We found showed slightly higher infectivity than a similar to compete in using Angiotensin-converting enzyme 2 (ACE2) BHK21-ACE2 cell line. However, significantly...
MAVS is an adapter protein involved in RIG-I-like receptor (RLR) signaling mitochondria, peroxisomes, and mitochondria-associated ER membranes (MAMs). However, the role of glucose metabolism RLR cross-regulation how these pathways are coordinated among organelles have not been defined. This study reports that action drives a switch from glycolysis to pentose phosphate pathway (PPP) hexosamine biosynthesis (HBP) through MAVS. We show peroxisomal responsible for flux shift into PPP type III...