A5080444407- Viral Infections and Immunology Research
- Animal Disease Management and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Research in Cotton Cultivation
- Nanoplatforms for cancer theranostics
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- Kruppel-like factors research
- COVID-19 Clinical Research Studies
- Ferroptosis and cancer prognosis
- Influenza Virus Research Studies
- Viral-associated cancers and disorders
- Fungal Infections and Studies
- Hepatitis C virus research
- interferon and immune responses
- Fungal and yeast genetics research
- Graphene and Nanomaterials Applications
- Vector-Borne Animal Diseases
- Cytomegalovirus and herpesvirus research
- Viral gastroenteritis research and epidemiology
- Cancer Immunotherapy and Biomarkers
- Fermentation and Sensory Analysis
- Cardiac Arrhythmias and Treatments
Wuhan University
2015-2025
Nanjing University of Posts and Telecommunications
2024
Sir Run Run Shaw Hospital
2023
Zhejiang University
2023
East China University of Science and Technology
2023
State Key Laboratory of Virology
2018-2022
Wuhan Institute of Virology
2022
Institute of Virology of the Slovak Academy of Sciences
2022
Guizhou University
2021
Harbin Institute of Technology
2020
Middle East respiratory syndrome coronavirus (MERS-CoV) and several bat coronaviruses use dipeptidyl peptidase-4 (DPP4) as an entry receptor1-4. However, the receptor for NeoCoV-the closest known MERS-CoV relative found in bats-remains unclear5. Here, using a pseudotype virus assay, we that NeoCoV its close relative, PDF-2180, can efficiently bind to specific angiotensin-converting enzyme 2 (ACE2) orthologues and, less favourably, human ACE2 receptors through their receptor-binding domains...
The prevalence of COVID-19 has caused global dysfunction in terms public health, sustainability, and socio-economy. While vaccination shows potential containing the spread, development surfaces that effectively reduces virus transmission infectivity is also imperative, especially amid early stage pandemic. However, most virucidal are operated under harsh conditions, making them impractical or potentially unsafe for long-term use. Here, it reported laser-induced graphene (LIG) without any...
In recent years, biological research involving human cell lines has been rapidly developing in China. However, some of the are not authenticated before use. Therefore, misidentified and/or cross-contaminated unfortunately commonplace. this study, we present a comprehensive investigation cross-contamination and misidentification for panel 278 from 28 institutes China by using short tandem repeat profiling method. By comparing DNA profiles with bank databases ATCC DSMZ, total 46.0% (128/278)...
Mediator of IRF3 activation (MITA), also known as stimulator interferon genes (STING), plays a vital role in the innate immune responses to cytosolic dsDNA. The trafficking MITA from ER perinuclear vesicles is necessary for its downstream molecules, which lead production interferons and pro-inflammatory cytokines. However, exact mechanism remains elusive. Here, we report that transmembrane emp24 protein transport domain containing 2 (TMED2) potentiates DNA virus-induced signaling....
Trichoderma harzianum is a well-known biological control agent (BCA) that effective against variety of plant pathogens. In previous studies, we found T. T4 could effectively the gray mold in tomatoes caused by Botrytis cinerea. However, research on its biocontrol mechanism not comprehensive, particularly regarding mycoparasitism. this study, order to further investigate mycoparasitism T4, transcriptomic sequencing and real-time fluorescence quantitative PCR (RT-qPCR) were used identify...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still epidemic around the world. manipulation of SARS-CoV-2 restricted to biosafety level 3 laboratories (BSL-3). In this study, we developed a ΔN-GFP-HiBiT replicon delivery particles (RDPs) encoding dual reporter gene, GFP-HiBiT, capable producing both GFP signal and luciferase activities. Through optimal selection GFP-HiBiT demonstrated superior stability convenience for antiviral evaluation. Additionally, established RDP...
Side effects of radiation therapy (RT) remain the most challenging issue for pancreatic cancer treatment. Cerium oxide nanoparticles (CONPs) are currently being tested in pre-clinical trials as an adjuvant to sensitize cells RT and protect normal tissues from harmful side effects. CONPs were not able significantly affect RT-induced DNA damage cells, thereby ruling out sensitization through increased mitotic catastrophe. However, activation c-Jun terminal kinase (JNK), a key driver apoptosis,...
Abstract Objectives Cryptococcus heimaeyensis S20 is found in Antarctica and can produce exopolysaccharides (CHEPS). Here, we explore the anti‐tumour effects of CHEPS on non‐small cell lung cancer (NSCLC). Materials methods Cell viability was assessed by CCK8 colony formation assays. Flow cytometry used to analyse cycle, apoptosis reactive oxygen species (ROS). autophagy detected EGFP‐LC3 puncta assay, Lyso‐Tracker Red staining transmission electron microscopy. mRNA protein levels were...
Abstract Concurrent epidemics of respiratory viruses provide avenues for intricate virus-virus interactions, yet how molecular-level viral interactions patterns shape ecology and epidemic dynamics remain enigmatic. Here, we present real-world virus crosstalk by comprehensively analyzing diagnostic data from a large cohort (37,415 illness cases pre-COVID-19 pandemic, 22,239 thereafter), mainly infants/toddlers, sourced the same local hospital. Such high-risk group allowed us to examine...
Abstract Worldwide, countless deaths have been caused by the coronavirus disease 2019. In addition to virus variants, an increasing number of fatal fungal infections reported, which further exacerbates scenario. Therefore, development porous surfaces with both antiviral and antimicrobial capacities is urgent need. Here, a cost‐effective, nontoxic, metal‐free strategy reported for surface engineering laser‐induced graphene (LIG). The authors covalently engineer potential LIG from −14 ≈+35 mV...
The quality of cellular products used in biological research can directly impact the ability to obtain accurate results. Epstein-Barr virus (EBV) is a latent that spreads extensively worldwide, and cell lines experiments may carry EBV pose an infection risk. presence single line contaminate other same laboratory, affecting experimental We developed three detection systems: (1) polymerase chain reaction (PCR)-based system, (2) recombinase amplification (RPA)-based (3) combined RPA-lateral...
Krüppel-like factor 8 (KLF8) regulates critical gene transcription and cellular events associated with cancer. However, the role of KLF8 in cancer remains largely unknown. Here, we report a surprisingly novel for DNA repair breast cells. Comet, clonogenic, WST-1 assays showed that expression is required protecting human cells from doxorubicin-induced damage cell death. Western blotting indicated overexpression ectopic attenuated levels marker γH2A.X doxorubicin-treated PARP-1(+/+) but not...
ABSTRACT Viral infection and replication are affected by host cell heterogeneity, but the mechanisms underlying effects remain unclear. Using single-cell analysis, we investigated of including size, inclusion, cycle, on foot-and-mouth disease virus (FMDV) (acute persistent infections) replication. We detected various viral genome levels in FMDV-infected cells. Large cells with a high number inclusions generated more RNA copies protein higher proportion infectious than other Additionally,...
Food-and-mouth disease virus (FMDV) is a highly contagious that seriously threatens the development of animal husbandry. Although persistent FMDV infection can dramatically worsen situation, mechanisms involved in remain unclear. In present study, we identified presence evolved cells persistently FMDV-infected cell line. These exhibited resistance to parent and re-established when infected with FMDV-Op (virus supernatant lines), emphasizing decisive role host establishment infection. Using...
Hepatic stellate cell (HSC) activation is a central event during hepatic fibrosis. Aerobic glycolysis one of its metabolic hallmarks. Blocking novel therapeutic option for liver This study investigated the effects costunolide, natural product demonstrated to have hepatoprotective effects, on HSC and glycolysis.Primary HSCs were isolated from rats cultured through 5 6 passages. Cell viability, markers, glycolytic metabolism examined in primary using various cellular molecular approaches.At 30...
Summary Middle East Respiratory Syndrome coronavirus (MERS-CoV) and several bat coronaviruses employ Dipeptidyl peptidase-4 (DPP4) as their functional receptors 1–4 . However, the receptor for NeoCoV, closest MERS-CoV relative yet discovered in bats, remains enigmatic 5 In this study, we unexpectedly found that NeoCoV its close relative, PDF-2180-CoV, can efficiently use some types of Angiotensin-converting enzyme 2 (ACE2) and, less favorably, human ACE2 entry. The two viruses spikes’ S1...
Foot-and-mouth disease virus (FMDV) is a single-stranded picornavirus that causes economically devastating in even-hooved animals. There has been little research on the function of host cells during FMDV infection. We aimed to shed light key factors associated with replication acute found HDAC1 overexpression induced upregulation RNA and protein levels. Activation AKT-mammalian target rapamycin (mTOR) signaling pathway using bpV(HOpic) or SC79 also promoted replication. Furthermore, short...
// Tianshu Li 1, 2 , Heng Lu 1 Debarati Mukherjee Satadru K. Lahiri Chao Shen 3 Lin Yu Jihe Zhao Burnett School of Biomedical Sciences, University Central Florida College Medicine, Orlando, FL, USA Department Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, Life Wuhan University, Wuhan, China Correspondence to: Zhao, e-mail: Jihe.Zhao@ucf.edu Keywords: KLF8, EGFR, microRNA141, invasion and metastasis, breast cancer Received: March 17, 2015 Accepted: May 14,...