A5100359915- Congenital heart defects research
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Congenital Heart Disease Studies
- Renal and related cancers
- Reproductive Biology and Fertility
- Aortic Disease and Treatment Approaches
- Bacterial Identification and Susceptibility Testing
- Soybean genetics and cultivation
- Adipose Tissue and Metabolism
- Aortic aneurysm repair treatments
- RNA modifications and cancer
- Infective Endocarditis Diagnosis and Management
- Antiplatelet Therapy and Cardiovascular Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Tracheal and airway disorders
- Healthcare and Venom Research
- Atherosclerosis and Cardiovascular Diseases
- Plant Genetic and Mutation Studies
- Antimicrobial Resistance in Staphylococcus
- Retinal Diseases and Treatments
- Nuclear Structure and Function
- Coronary Interventions and Diagnostics
- Glaucoma and retinal disorders
- Genomics and Chromatin Dynamics
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2025
Nanjing Medical University
2024
Fu Wai Hospital
2018-2024
Children's Hospital of Fudan University
2020-2023
Shandong Agricultural University
2022-2023
Shanghai Veterinary Research Institute
2023
Chinese Academy of Agricultural Sciences
2023
University of Science and Technology of China
2023
Guangdong Medical College
2023
The People's Hospital of Guangxi Zhuang Autonomous Region
2023
Abstract The beating heart possesses the intrinsic ability to adapt cardiac output changes in mechanical load. century-old Frank–Starling law and Anrep effect have documented that stretching during diastolic filling increases its contractile force. However, molecular mechanotransduction mechanism impact on health disease remain elusive. Here we show mechanically activated Piezo1 channel converts stretch of cardiomyocytes into Ca 2+ reactive oxygen species (ROS) signaling, which critically...
Cardiac outflow tract (OFT) is a major hotspot for congenital heart diseases. A thorough understanding of the cellular diversity, transitions, and regulatory networks normal OFT development essential to decipher etiology malformations. We performed single-cell transcriptomic sequencing 55,611 mouse cells from three developmental stages that generally correspond early, middle, late remodeling septation. Known such as endothelial-to-mesenchymal transition, have been recapitulated. In...
Abstract Thoracic aortic aneurysm and dissection (TAAD) is a life-threatening condition characterized by medial layer degeneration of the thoracic aorta. A thorough understanding regulator changes during pathogenesis essential for medical therapy development. To delineate cellular molecular development TAAD, we performed single-cell RNA sequencing cells from β-aminopropionitrile-induced TAAD mouse models at three time points that spanned early to advanced stages disease. Comparative analyses...
Hypertrophic cardiomyopathy (HCM) is the most common cardiac genetic disorder characterized by cardiomyocyte hypertrophy and fibrosis. Pathological remodeling in myocardium of HCM patients may progress to heart failure. An in-depth elucidation lineage-specific changes pathological pivotal for development therapies mitigate progression. Here, we performed single-nucleus RNA-seq tissues from or healthy donors conducted spatial transcriptomic assays on tissue sections patients. Unbiased...
Preimplantation embryo arrest (PREMBA) is a common cause of female infertility and recurrent failure assisted reproductive technology. However, the genetic basis PREMBA largely unrevealed. Here, using whole-exome sequencing data from 606 women experiencing compared with 2,813 controls, we performed population gene-based burden test identified candidate gene, karyopherin subunit α7 (KPNA7). In vitro studies showed that sequence variants reduced KPNA7 protein levels, impaired capacity for...
In the fission yeast Schizosaccharomyces pombe, centromeres of each chromosome are clustered together and attached to nuclear envelope near site spindle pole body during interphase. The mechanism functional importance this arrangement chromosomes poorly understood. paper, we identified a novel protein, Csi1, that localized centromere attachment interacted with both inner SUN domain protein Sad1 centromeres. Both Csi1 mutants exhibited clustering defects in high percentage cells. also...
Abstract Adipose-derived mesenchymal stem cells (ADSCs) show considerable promise for clinical applications in regenerative medicine. We performed a large-scale single-cell transcriptomic sequencing of 24,358 cultured human ADSCs from three donors. provide high-quality dataset, which would be valuable resource dissecting the intrapopulation heterogeneity as well interrogating lineage priming patterns any interested lineages at resolution.
Pathogens identification is critical for the proper diagnosis and precise treatment of infective endocarditis (IE). Although blood valve cultures are gold standard IE pathogens detection, many cases culture-negative, especially in patients who had received long-term antibiotic treatment, has therefore become a major challenge clinic. Metagenomic sequencing can provide both information on pathogenic strain susceptibility profile patient samples without culturing, offering powerful method to...
Identification of the underlying pathogens infective endocarditis (IE) is critical for precision therapy.We evaluated a metagenomic method with next-generation sequencing (NGS) direct detection from resected valves 44 IE patients and seven rejected according to modified Duke criteria.NGS displayed sensitivity, specificity, positive predictive values negative 97.6%, 85.7%, 85.7% compared 46.2%, 100%, 12.5% blood culture 17.1%, 17.1% valve 51.4%, 26.1% Gram staining, respectively.NGS technique...
Fertilization involves a series of molecular events immediately following egg-sperm fusion; Ca2+ oscillations are the earliest signaling event, and they initiate downstream reactions including pronucleus formation. Successful human reproduction requires normal fertilization. In clinical IVF or ICSI attempts, some infertile couples suffer from recurrent fertilization failure. However, genetic reasons for failure largely unknown. Here, we recruited several diagnosed with even though their...
Abstract Background Cardiac myxoma (CM) is the most common (58%–80%) type of primary cardiac tumours. Currently, there a need to develop medical therapies, especially for patients not physically suitable surgeries. However, mechanisms that shape tumour microenvironment (TME) in CM remain largely unknown, which impedes development targeted therapies. Here, we aimed dissect TME at single‐cell and spatial resolution. Methods We performed transcriptomic sequencing Visium CytAssist (ST) assays on...
Rationale: Transposition of the great arteries (TGA) is one most severe types congenital heart diseases. Understanding clinical characteristics and pathogenesis TGA is, therefore, urgently needed for patient management this disease. However, genetic cause underlying remain largely unexplored. Objective: We sought to systematically examine isolated nonsyndromic TGA. Methods Results: recruited 249 patients with (66 family trios) performed whole-exome sequencing. The incidence patent ductus...
Summary Soybean cyst nematode (SCN, Heterodera glycines) is the most devastating pest affecting soybean production worldwide. SCN resistance requires both GmSHMT08 and GmSNAP18 in ‘Peking’‐type . Here, we describe molecular interaction between GmSNAP18, which potentiated by a pathogenesis‐related protein GmPR08‐Bet VI. Like , VI expression was induced response to its overexpression decreased cysts 65% infected transgenic roots. Overexpression of did not have an effect on when two...
Cancer-associated lymphedema frequently occurs following lymph node resection for cancer treatment. However, we still lack effective targeted medical therapies the treatment or prevention of this complication. An in-depth elucidation cellular alterations in subcutaneous adipose tissues is essential development. We performed single-cell RNA sequencing 70,209 cells stromal vascular fraction from patients and healthy donors. Four subpopulations adipose-derived (ASCs) were identified. Among...
Resource27 September 2021Open Access Transparent process Single-cell transcriptomic landscape of cardiac neural crest cell derivatives during development Wen Chen orcid.org/0000-0001-6658-1926 State Key Laboratory Cardiovascular Disease, Beijing for Molecular Diagnostics Diseases, Center Medicine, Fuwai Hospital, National Chinese Academy Medical Sciences and Peking Union College, Beijing, China Search more papers by this author Xuanyu Liu Corresponding Author [email protected]...
Abstract Aortic root aneurysm is a potentially life‐threatening condition that may lead to aortic rupture and often associated with genetic syndromes, such as Marfan syndrome (MFS). Although studies MFS animal models have provided valuable insights into the pathogenesis of aneurysms, this understanding transcriptomic epigenomic landscape in human tissue remains incomplete. This knowledge gap has impeded development effective targeted therapies. Here, study performs first integrative analysis...
Soybean [Glycine max (L.) Merr.] seed is a valuable source of protein and oil worldwide. Traditionally, the natural variations were heavily used in conventional soybean breeding programs to select desired traits. However, traditional plant encumbered with low frequencies spontaneous mutations. In mutation breeding, genetic from induced mutations provide abundant sources alterations important traits; this facilitated development germplasm modified composition traits meet different needs end...
Abstract Left ventricular hypertrophy and fibrosis are major risk factors for heart failure, which require timely effective treatment. Genetic therapy has been shown to ameliorate hypertrophic cardiac damage. In this study, it is found that in mice, the dopamine D5 receptor (D5R) expression left ventricle (LV) progressively decreases with worsening of transverse aortic constriction‐induced hypertrophy. Then, a reversible treatment Drd5 nucleic acids delivered by tobramycin‐based...
Foot-and-mouth disease virus (FMDV) is a single-stranded picornavirus that causes economically devastating in even-hooved animals. There has been little research on the function of host cells during FMDV infection. We aimed to shed light key factors associated with replication acute found HDAC1 overexpression induced upregulation RNA and protein levels. Activation AKT-mammalian target rapamycin (mTOR) signaling pathway using bpV(HOpic) or SC79 also promoted replication. Furthermore, short...
Primary Sjögren's syndrome (pSS) is a complex autoimmune disease characterized by lymphocytic infiltration and exocrine dysfunction, particularly affecting the salivary gland (SG). We employed single-cell RNA sequencing to investigate cellular heterogeneity in 11 patients with pSS 5 non-SS controls. Notably, exhibited downregulated SOX9 myoepithelial cells, potentially associated impaired epithelial regeneration. An expanded ACKR1+ endothelial subpopulation suggested role facilitating...