A5101462707- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Systemic Lupus Erythematosus Research
- Pancreatic and Hepatic Oncology Research
- Radiomics and Machine Learning in Medical Imaging
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Cell Adhesion Molecules Research
- COVID-19 Clinical Research Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Mass Spectrometry Techniques and Applications
- Gallbladder and Bile Duct Disorders
- MicroRNA in disease regulation
- Pharmacological Effects of Natural Compounds
- Scheduling and Optimization Algorithms
- T-cell and B-cell Immunology
- Industrial Technology and Control Systems
- Advanced Manufacturing and Logistics Optimization
- Plant-derived Lignans Synthesis and Bioactivity
- Cancer, Lipids, and Metabolism
- Reproductive System and Pregnancy
- Long-Term Effects of COVID-19
- Salivary Gland Disorders and Functions
- Cancer-related molecular mechanisms research
Westlake University
2020-2024
China Aerospace Science and Industry Corporation (China)
2023-2024
Anhui Medical University
2012-2024
State Grid Nanjing Power Supply Company (China)
2019-2024
Zhujiang Hospital
2011-2024
Southern Medical University
2011-2024
China Medical University
2024
Longhua Hospital Shanghai University of Traditional Chinese Medicine
2024
Shanghai University of Traditional Chinese Medicine
2024
Hubei University Of Economics
2023-2024
The utility of the urinary proteome in infectious diseases remains unclear. Here, we analyzed and metabolome urine serum samples from patients with COVID-19 healthy controls. Our data show that proteins effectively classify by severity. We detect 197 cytokines their receptors urine, but only 124 using TMT-based proteomics. decrease ESCRT complex correlates active SARS-CoV-2 replication. downregulation CXCL14 severe cases positively blood lymphocyte counts. Integrative multiomics analysis...
Three-dimensional reconstruction visualization technology (3D-RVT) is an important tool in the preoperative assessment of patients undergoing liver resection. However, it not clear whether this technique can improve short-term and long-term outcomes with hepatocellular carcinoma (HCC) compared two-dimensional (2D) imaging.
Epithelial ovarian cancer (EOC) is a deadly disease with limited diagnostic biomarkers and therapeutic targets. Here we conduct comprehensive proteomic profiling of tissue plasma samples from 813 patients different histotypes regimens, covering the expression 10,715 proteins. We identify eight proteins associated tumor malignancy in specimens, which are further validated as potential circulating plasma. Targeted proteomics assays developed for 12 7 blood proteins, machine learning models...
To address the increasing need for detecting and validating protein biomarkers in clinical specimens, mass spectrometry (MS)-based targeted proteomic techniques, including selected reaction monitoring (SRM), parallel (PRM), massively data-independent acquisition (DIA), have been developed. For optimal performance, they require fragment ion spectra of peptides as prior knowledge. In this report, we describe a MS pipeline spectral resource to support proteomics studies human tissue samples....
Metabolic syndrome (MetS) is a complex metabolic disorder with global prevalence of 20%–25%. Early identification and intervention would help minimize the burden on healthcare systems. Here, we measured over 400 proteins from ∼20,000 proteomes using data-independent acquisition mass spectrometry for 7,890 serum samples longitudinal cohort 3,840 participants two follow-up time points 10 years. We then built machine-learning model predicting risk developing MetS within Our model, composed 11...
Circular RNA (circRNA), a type of non‑coding RNA, plays regulatory role in biological processes. The special loop structure circRNA makes it highly stable and specific diseased tissues cells, especially tumors. Competing endogenous RNAs (ceRNAs) compete for the binding microRNA (miRNA) at sites thus regulate gene expression. ceRNAs play an important various diseases are currently recognized as most prominent mechanism action circRNAs. circRNAs can modulate proliferation, migration, invasion...
SUMMARY Severe COVID-19 patients account for most of the mortality this disease. Early detection and effective treatment severe remain major challenges. Here, we performed proteomic metabolomic profiling sera from 46 53 control individuals. We then trained a machine learning model using measurements training cohort 18 non-severe 13 patients. The correctly classified with an accuracy 93.5%, was further validated ten independent patients, seven which were classified. identified molecular...
In this study, a composite coating for corrosion protection was successfully prepared on Q235 steel under neutral conditions by means of the DC electrodeposition technique. According to results scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) and electrochemical tests, effects different additions yttrium oxide nanoparticles in electrolyte surface morphology, phase structure resistance nickel-molybdenum alloys were analyzed, deposition...
Efficient peptide and protein identifications from data-independent acquisition mass spectrometric (DIA-MS) data typically rely on a project-specific spectral library with suitable size. Here, we describe subLib, computational strategy for optimizing the specific DIA set based comprehensive library, requiring preliminary analysis of set. Compared pan-human strategy, subLib achieved 41.2% increase in precursor 35.6% group test six colorectal tumor samples. We also applied this to 389...
RT-PCR is the primary method to diagnose COVID-19 and also used monitor disease course. This approach, however, suffers from false negatives due RNA instability poses a high risk medical practitioners. Here, we investigated potential of using serum proteomics predict viral nucleic acid positivity during COVID-19. We analyzed proteome 275 inactivated samples 54 out 144 patients shortlisted 42 regulated proteins in severe group 12 non-severe group. Using these several key clinical indexes,...
This study compared the effects of hot air drying (HAD) and vacuum (VD) on physicochemical qualities lily (Lilium lancifolium Thunb.) bulbs. The results showed that VD treatment effectively reduced browning scales with a total color difference (ΔE) 42–73% lower than those HAD group. Two treatments enhanced phenolic content (TPC) bulbs effectively. TPC increased rising temperatures, under isothermal conditions, group was 1.1–1.6-fold higher Five typical compounds were identified in As...
A comprehensive pan-human spectral library is critical for biomarker discovery using mass spectrometry (MS)-based proteomics. DPHL v.1, a previous built from 1,096 data-dependent acquisition (DDA) MS data of 16 human tissue types, allows quantifying 10,943 proteins. Here, we generated v.2 1,608 DDA-MS data. The included 586 acquired 18 while 1,022 files were derived v.1. thus comprises 24 sample including several cancer types (lung, breast, kidney, and prostate cancer, among others). We four...
B cells are recognized as key participants in various autoimmune diseases, including systemic lupus erythematosus (SLE). Although sets of transcription factors and cytokines known to regulate cell differentiation, the roles microRNAs poorly understood. Our previous study proved that microRNA-326 (miR-326) was markedly upregulated SLE patients; however, biological function miR-326 during pathogenesis remained unknown. In this study, we found overexpression MRL/lpr mice led hyperactivity...
This study aims to investigate the response profiles of vitamin E and carotenoids on transcription metabolic levels sweet corn seedlings under temperature stress. The treated temperatures were set as 10 °C (low temperature, LT), 25 (control, CK), 40 (high HT) for seedlings. gene expression biosynthesis pathways analyzed by real time quantitative polymerase chain reaction (RT-qPCR), composition high performance liquid chromatography (HPLC). Results showed that gradually accumulated in LT...
Primary Sjögren's syndrome (pSS) is a complex autoimmune disease characterized by lymphocytic infiltration and exocrine dysfunction, particularly affecting the salivary gland (SG). We employed single-cell RNA sequencing to investigate cellular heterogeneity in 11 patients with pSS 5 non-SS controls. Notably, exhibited downregulated SOX9 myoepithelial cells, potentially associated impaired epithelial regeneration. An expanded ACKR1+ endothelial subpopulation suggested role facilitating...