A5082492592- Cervical Cancer and HPV Research
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Genital Health and Disease
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Reproductive tract infections research
- Urinary and Genital Oncology Studies
- Cancer Genomics and Diagnostics
- Endometrial and Cervical Cancer Treatments
- Kruppel-like factors research
- Bladder and Urothelial Cancer Treatments
- RNA modifications and cancer
- Colorectal and Anal Carcinomas
- Viral-associated cancers and disorders
- Cancer-related Molecular Pathways
- Hepatitis B Virus Studies
- Global Cancer Incidence and Screening
- Urological Disorders and Treatments
- Hepatitis C virus research
- Genetic factors in colorectal cancer
- Head and Neck Cancer Studies
- Telomeres, Telomerase, and Senescence
- Genomic variations and chromosomal abnormalities
Cancer Center Amsterdam
2018-2025
Amsterdam University Medical Centers
2018-2025
Dutch Cancer Society
2022-2025
Vrije Universiteit Amsterdam
2008-2024
Amsterdam University of Applied Sciences
2021-2023
Norwegian Womens Public Health Association
2023
Amsterdam UMC Location Vrije Universiteit Amsterdam
2010-2022
University of Alberta
2002-2020
Amsterdam Public Health
2016
University Hospital and Clinics
2013-2015
Objective Using highly sensitive polymerase chain reaction ( PCR ) techniques, we reanalysed all cervical carcinomas CC s) found to be human papillomavirus HPV )‐negative by Hybrid Capture 2 HC 2) determine the prevalence of true ‐negativity. We also evaluated characteristics patients with tumours confirmed Design Observational study. Setting Barcelona, Spain. Population A cohort 136 women (32 adenocarcinomas, 104 squamous cell carcinomas) who had pre‐treatment testing. Methods All negative...
16S rRNA genes from a range of aerobic chemoorganotrophic, alkaliphilic soda lake Bacteria and Archaea have been sequenced subjected to phylogenetic analysis. Gram-negative alkaliphiles were found be confined the γ3 subdivision Proteobacteria, with many isolates related Halomonas/Deleya group. Gram-positive in both high % G + C low divisions lineage, being Bacillus group, others Arthrobacter spp. Alkaliphilic relatively closely members genera Natronococcus Natronobacterium. An anaerobic,...
Most of the phosphatidylethanolamine (PE) in mammalian cells is synthesized by two pathways, CDP-ethanolamine pathway and phosphatidylserine (PS) decarboxylation pathway, final steps which operate at spatially distinct sites, endoplasmic reticulum mitochondria, respectively. We investigated importance mitochondrial for PE synthesis mice generating lacking PS decarboxylase activity. Disruption Pisd resulted lethality between days 8 10 embryonic development. Electron microscopy Pisd-/- embryos...
Abstract Background A substantial number of microRNAs (miRNAs) is subject to epigenetic silencing in cancer. Although tumour suppressor genes an important feature cervical cancer, little known about miRNAs. Since DNA methylation-based hsa-miR-124 occurs various human cancers, we studied the frequency and functional effects methylation carcinogenesis. Results Quantitative MSP analysis all 3 loci encoding mature (hsa-miR-124-1/-2/-3) showed cancer cell lines SiHa, CaSki HeLa as well late...
The natural history of cancers associated with virus exposure is intriguing, since only a minority human tissues infected these viruses inevitably progress to cancer. However, the molecular reasons why infection controlled or instead progresses subsequent stages tumorigenesis are largely unknown. In this article, we provide first complete DNA methylomes double-stranded cancer that might important clues help us understand described process. Using bisulfite genomic sequencing multiple clones,...
Background: Cervical carcinogenesis is initiated by infection with high-risk (i.e., carcinogenic) human papillomavirus (HPV) types. The subsequent progression from premalignant cervical intraepithelial neoplasia (CIN) to invasive cancer driven both genetic and epigenetic processes. We assessed the role of gene encoding adhesion molecule tumor suppressor in lung 1 (TSLC1) this progression. Methods: analyzed TSLC1 expression real-time quantitative reverse transcription–polymerase chain...
Screening women for high-grade cervical intraepithelial neoplasia or cancer (CIN3(+)) by high-risk human papillomavirus (hrHPV) testing has as side-effect the detection of hrHPV-positive without clinically relevant lesions. Here, we developed an objective assay assessing methylation status promoter regions CADM1 and MAL to triage CIN3(+).In a training set (51 with CIN3(+) 224 CIN2(+)), panels consisting one four quantitative methylation-specific PCR (qMSP) assays...
Abstract Primary testing for human papillomavirus (HPV) in cervical screening requires triage to differentiate women with transient infection from those persistent who require more intensive management given their risk (pre)cancer. In this study, the clinical performance of a novel methylation marker FAM19A4 high-risk (hr)HPV-positive was evaluated. Using training-validation set approach, we analyzed quantitative methylation-specific PCR (qMSP). The training comprised hrHPV-positive scrapes...
Combined detection of cell adhesion molecule 1 (CADM1) and T-lymphocyte maturation-associated protein (MAL) promoter methylation in cervical scrapes is a promising triage strategy for high-risk human papillomavirus (hrHPV)-positive women. Here, CADM1 MAL DNA levels were analysed hrHPV-positive women with no underlying high-grade disease, intraepithelial neoplasia (CIN) cancer. first related to CIN-grade the corresponding biopsy second stratified by presence 'normal' or 'abnormal' cytology as...
DNA methylation analysis of cancer-related genes is a promising tool for HPV-positive women to identify those with cervical (pre)cancer (CIN3+) in need treatment. However, clinical performance markers can be influenced by the sample type utilized. We describe multiplex quantitative methylation-specific PCR that targets FAM19A4 and mir124-2 loci, detect CIN3+ using both lavage- brush self-samples.We determined thresholds classification training sets comprising lavage self-samples 182...
Abstract The risk of vulvar squamous cell carcinoma (VSCC) in patients with high‐grade intraepithelial neoplasia (VIN) is considered lower lesion (HSIL) compared to differentiated VIN (dVIN), but studies are limited. Our study investigated both the incidence and cumulative VSCC HSIL dVIN separately. A database women diagnosed between 1991 2011 was constructed data from Dutch Pathology Registry (PALGA). European standardized rate (ESR) were calculated, stratified for dVIN. effects type (HSIL...
Cervical screening can prevent cancer by detection and treatment of cervical intraepithelial neoplasia grade 2 or 3 (CIN2/3). Screening also results in considerable overtreatment because many CIN2/3 lesions show spontaneous regression when left untreated. In this multicenter longitudinal cohort study women with untreated CIN2/3, the prognostic value FAM19A4/miR124-2 methylation was evaluated for clinical regression.Women were prospectively followed 24 months. Surgical excision replaced a...
Abstract Purpose: Biomarker detection in urine offers a potential solution to increase effectiveness of cervical cancer screening programs by attracting nonresponders. In this prospective study, the presence high-risk human papillomavirus (hrHPV) DNA and performance methylation analysis was determined for high-grade intraepithelial neoplasia (CIN2/3) urine, compared with paired cervicovaginal self-samples clinician-taken scrapes. Experimental Design: A total 587 samples were included from...
Cell-free DNA (cfDNA) can be isolated and sequenced from blood and/or urine of cancer patients. Conventional short-read sequencing lacks deployability speed biased for short cfDNA fragments. Here, we demonstrate that with Oxford Nanopore Technologies (ONT) achieve delivery genomic fragmentomic data liquid biopsies. Copy number aberrations fragmentation patterns determined in less than 24 h sample collection. The tumor-derived fraction calculated plasma lung patients bladder was highly...
DNA methylation is proposed as a novel biomarker able to monitor molecular events in human papillomavirus (HPV) infection pathophysiology, enabling the distinction between HPV-induced lesions with regression potential from those that may progress HPV-related cancer.
Hemidesmosomes (HDs) are stable anchoring structures that mediate the link between intermediate filament cytoskeleton and cell substratum. We investigated contribution of various segments β4 integrin cytoplasmic domain in formation HDs transient transfection studies using immortalized keratinocytes derived from an epidermolysis bullosa patient deficient expression. found expression wild-type restored ability β4-deficient cells to form distinct domains NH2- COOH-terminal regions required for...