A5075088009- Hormonal Regulation and Hypertension
- Ion Transport and Channel Regulation
- Receptor Mechanisms and Signaling
- Renin-Angiotensin System Studies
- Nitric Oxide and Endothelin Effects
- Electrolyte and hormonal disorders
- Birth, Development, and Health
- Sodium Intake and Health
- Renal function and acid-base balance
- Blood Pressure and Hypertension Studies
- Eicosanoids and Hypertension Pharmacology
- Diet and metabolism studies
- Renal and related cancers
- Pregnancy and preeclampsia studies
- Pancreatic function and diabetes
- Neuropeptides and Animal Physiology
- Neuroendocrine regulation and behavior
- Adipose Tissue and Metabolism
- Ion channel regulation and function
- Amino Acid Enzymes and Metabolism
- Caveolin-1 and cellular processes
- Diabetes Treatment and Management
- Adenosine and Purinergic Signaling
- Acute Kidney Injury Research
- MicroRNA in disease regulation
George Washington University
2016-2025
University of Maryland, Baltimore
2013-2025
Children's National
2009-2022
Kerala Forest Research Institute
2022
Kerala State Council for Science, Technology and Environment
2022
GW Medical Faculty Associates
2016-2022
Georgetown University Medical Center
2004-2021
Georgetown University
2007-2021
University of Virginia
2004-2021
University of Chinese Academy of Sciences
2021
We have utilized the polymerase chain reaction technique to selectively amplify a G protein-coupled receptor cDNA from rat kidney proximal convoluted tubule mRNA, which exhibits high homology with previously cloned serotonin receptors. Sequencing of full-length clone isolated hippocampal library revealed an open reading frame 1,212 base pairs encoding 404-residue protein seven hydrophobic regions predicted represent transmembrane-spanning domains. Within transmembrane regions, this was found...
Extracellular vesicles (EVs) carry signals within or at their limiting membranes, providing a mechanism by which cells can exchange more complex information than what was previously thought. In addition to mRNAs and microRNAs, there are DNA fragments in EVs. Solexa sequencing indicated the presence of least 16434 genomic (gDNA) EVs from human plasma. Immunofluorescence study showed direct evidence that acridine orange-stained EV DNAs could be transferred into localize inside nuclear...
Abstract Background Diabetic cardiomyopathy (DCM) is a serious complication in patients with type 1 diabetes mellitus (T1DM), which still lacks adequate therapy. Irisin, cleavage peptide off fibronectin III domain-containing 5, has been shown to preserve cardiac function ischemia–reperfusion injury. Whether or not irisin plays cardioprotective role DCM known. Methods and results T1DM was induced by multiple low-dose intraperitoneal injections of streptozotocin (STZ). Our current study showed...
Doxorubicin is a powerful chemotherapeutic agent for cancer, whose use limited due to its potential cardiotoxicity. Semaglutide (SEMA), novel analog of glucagon-like peptide-1 (GLP-1), has received widespread attention the treatment diabetes. However, increasing evidence highlighted therapeutic benefits on cardiac function. Therefore, objective this study was examine efficacy semaglutide in ameliorating doxorubicin-induced
Essential hypertension has a heritability as high 30–50%, but its genetic cause(s) not been determined despite intensive investigation. The renal dopaminergic system exerts pivotal role in maintaining fluid and electrolyte balance participates the pathogenesis of hypertension. In hypertension, ability dopamine D 1 -like agonists to increase urinary sodium excretion is impaired. A defective coupling between receptor G protein/effector enzyme complex proximal tubule kidney cause impaired...
To determine a renal tubular mechanism for the natriuretic effect of dopamine (DA) and DA-1 agonists, we measured Na(+)-H+ exchange activity (amiloride sensitive) in rat cortical brush-border membrane vesicles (BBMV). Renal tissues were preincubated with ligands before BBMV preparation to study absence added that may compete ion binding sites exchanger. DA agonist-inhibited was concentration time dependent. The inhibitory not due increased permeability, collapse proton gradient, or change...
Since dopamine produced by the kidney is an intrarenal regulator of sodium transport, abnormality dopaminergic system may be important in pathogenesis hypertension. In spontaneously hypertensive rat (SHR), spite normal renal production and receptor density, there defective transduction D1 signal proximal tubules, resulting decreased inhibition transport (Na+/H+ exchanger [NHE] Na+/K+ATPase activity) dopamine. To determine if impaired regulation NHE tubules related to hypertension, studies...
Dopamine-1 (DA-1) receptors have been found in renal tubular membranes which stimulate both adenylate cyclase and phospholipase-C activity. In cortical plasma membrane preparations the DA-1 agonist SKF 82526, forskolin NaF stimulated 2',5'-dideoxyadenosine inhibited basal Forskolin, NaF, dibutyryl-cyclic AMP had no effect on or activity these membranes. These studies indicate that stimulates activities independently. Phospholipase-C was also increased by nonhydrolyzable GTP analog,...
Long non-coding RNAs (lncRNAs) have been reported to be involved in the pathogenesis of cardiovascular disease (CVD), but whether circulating lncRNAs can serve as a coronary artery (CAD), biomarker is not known. The present study screened by microarray analysis plasma from CAD patients and control individuals found that 265 were differentially expressed. To find specific possible candidates, we used following criteria for 174 up-regulated lncRNAs: signal intensity ≥8, fold change >2.5...
Aging is a major risk factor for hypertension and cardiovascular disease. Accumulating evidence suggests that telomere length marker biological aging of the system. Telomere determined by genetic environmental factors. Studies in different racial populations are required to determine prognostic value diseases. The main objective this study was investigate association between leukocyte prognosis Chinese population. relative leukocytes quantitative PCR-based method 767 subjects: 379 healthy...
Background Exercise is a major nonpharmacological treatment for hypertension, but its underlying mechanisms are still not completely elucidated. Irisin, polypeptide containing 112 amino acids, which secreted mainly by skeletal muscle cells during exercise, exerts protective role in metabolic diseases, such as diabetes mellitus and obesity. Because of the close relationship between irisin we hypothesized that may play regulation blood pressure. Methods Results Blood pressures male...
Long non-coding RNAs (lncRNAs) have been found to be involved in the pathogenesis of coronary artery disease (CAD). However, it remains established whether or not circulating lncRNAs can serve as biomarkers CAD. Using a microarray-based lncRNA expression profiling, we 86 that were differentially expressed peripheral blood monocytes and plasma from 15 CAD patients control subjects. After choosing consistent criterion (average normalized intensity ≥7 with significance <0.005) confirmed by...
Both oxidative stress and inflammation are involved in the pathogenesis of contrast-induced nephropathy (CIN). Epigallocatechin-3-gallate (EGCG), a purified catechin from green tea, has antioxidant anti-inflammatory effects. However, it is unknown whether or not EGCG effective treating CIN. Our present study found that intravenous administration EGCG, either before just after establishment CIN, had protective effect, determined by normalization serum creatinine blood urea nitrogen levels,...
Circular RNAs (circRNAs) have been recently recognized as playing a role in the pathogenesis of vascular remodeling–related diseases by modulating functions miRNAs. However, interplay between circRNAs and proteins during remodeling remains poorly understood. Here, we investigated previously identified circRNA, circEsyt2, whose expression is known to be upregulated remodeling. Loss- gain-of‑function mutation analyses smooth muscle cells (VSMCs) revealed that circEsyt2 enhanced cell...
BackgroundWhile the adult mammalian heart undergoes only modest renewal through cardiomyocyte proliferation, boosting this process is considered a promising therapeutic strategy to repair cardiac injury. This study explored role and mechanism of dual-specificity tyrosine regulated kinase 1A (DYRK1A) in regulating cell cycle activation after myocardial infarction (MI).MethodsDYRK1A-knockout mice DYRK1A inhibitors were used investigate following MI. Additionally, we underlying mechanisms by...
Ischemic heart disease (IHD) is the leading cause of death among noncommunicable diseases worldwide, but data on current epidemiological patterns and associated risk factors are lacking.
Since dopamine receptors are important in the regulation of renal and cardiovascular function, we studied consequences disruption D3 receptor, a member family D2-like receptors, expressed proximal tubules juxtaglomerular cells. Systolic diastolic blood pressures were higher (approximately 20 mmHg) heterozygous homozygous than wild-type mice. An acute saline load increased urine flow rate sodium excretion to similar extent mice but increase was attenuated Renal renin activity much greater...
Dopamine is an important transmitter in the CNS and PNS, critically regulating numerous neuropsychiatric physiological functions. These actions of dopamine are mediated by five distinct receptor subtypes. Of these receptors, probably least understood terms functions D<sub>5</sub> subtype. To better understand role (DAR) normal physiology behavior, we have now used gene-targeting technology to create mice that lack this We find receptor-deficient viable fertile appear develop normally. No...