Seasonal variations in gut microbiota of small mammals and how they are influenced by environmental variables relatively poorly understood. We sampled 162 wild plateau pikas (Ochotona curzoniae) 4 seasons over 2 a half years recorded the air temperature, precipitation, nutrient content edible vegetation at sampling site. After conducting 16S rRNA shotgun metagenomic sequencing, we found that highest alpha diversity, relative abundance Firmicutes, simplest co-occurrence network occurred...

BackgroundWhile the adult mammalian heart undergoes only modest renewal through cardiomyocyte proliferation, boosting this process is considered a promising therapeutic strategy to repair cardiac injury. This study explored role and mechanism of dual-specificity tyrosine regulated kinase 1A (DYRK1A) in regulating cell cycle activation after myocardial infarction (MI).MethodsDYRK1A-knockout mice DYRK1A inhibitors were used investigate following MI. Additionally, we underlying mechanisms by...

Coronary heart disease (CHD) is closely related to gut microbiota, which may be significantly affected by ethnicity and the environment. Knowledge regarding microbiome of Tibetan CHD patients living in Qinghai-Tibet Plateau very limited. In this study, we characterised physiological parameters microbiota from 23 healthy Tibetans (HT), 18 patients, 12 with non-stenosis coronary (NCHD). We analysed alterations investigated relationship between these pathological indicators. found no changes...

Summary The previous studies have reported that the mammalian gut microbiota is a physiological consequence; nonetheless, factors influencing its composition and function remain unclear. In this study, to evaluate contributions of host environment microbiota, we conducted sequencing analysis 16S rDNA shotgun metagenomic DNA from plateau pikas yaks, two sympatric herbivorous mammals, further compared sequences in summer winter. results revealed both yaks harboured considerably more distinct...

Abstract Aims Exposure to maternal diabetes is associated with increased prevalence of hypertension in the offspring. The mechanisms underlying prenatal programming remain unclear. Because endoplasmic reticulum (ER) stress plays a key role vascular endothelial dysfunction hypertension, we investigated whether aberrant ER causes and high blood pressure offspring dams diabetes. Methods results Pregnant Sprague-Dawley rats were intraperitoneally injected streptozotocin (35 mg/kg) or citrate...

Gastrointestinal tract and dietary fiber (DF) are known to influence gut microbiome composition. However, the combined effect of segment long-term intake a high diet on pig microbiota metabolite profiles is unclear. Here, we applied 16S rRNA gene sequencing untargeted metabolomics investigate broad bean silage composition metabolites cecal jejunal in Durco × Bamei crossbred pigs. Twenty-four pigs were allotted four graded levels DF chow, content jejunum cecum collected. Our results...

Background: In lung adenocarcinoma (LUAD), the metabolism of amino acids (AAs) plays a crucial role in growth, infiltration, and metastasis tumor cells. Nevertheless, potential AA metabolism-associated genes (AAMRGs) to serve as prognostic indicators LUAD remains ambiguous. Thus, this study sought evaluate value AAMRGs patients. Methods: Herein, we extracted transcriptomic information from two key repositories, namely The Cancer Genome Atlas Program (TCGA) Gene Expression Omnibus....

Ovarian cancer is the leading cause of mortality due to gynecological malignancy, and vasculogenic mimicry (VM) formation correlated with poor prognosis. In a previous study, present authors observed that human chorionic gonadotropin (HCG) could promote VM in three-dimensional OVCAR-3 cell cultures. order investigate whether HCG ovarian vivo, role cells overexpressing or depleted gonadotropin, beta polypeptide 5 (CGB5, which fifth subunit β-HCG was identified as key part HCG) were injected...

Background: The crush and the culotte stenting were both reported to be effective for complex bifurcation lesion treatment. However, their comparative performance remains elusive. Methods: A total of 300 patients with coronary lesions randomly assigned (n = 150) primary endpoint was occurrence major adverse cardiac events (MACEs) at 12 months including death, myocardial infarction, stent thrombosis, target vessel revascularization. Index restenosis a secondary endpoint. surface integrals...

Interactions between species provide the basis for understanding coexisting mechanisms. The plateau pika (Ochotona curzoniae) and yak (Bos grunniens) are considered competitors because they have shared habitats consumed similar food on Qinghai–Tibetan Plateau more than 1 million years. Interestingly, population density of pikas increases with expansion subsequent overgrazing. To reveal underlying mechanism, we sequenced fecal microbial 16S rDNA from both sympatric allopatric yaks. Our...

The mechanisms responsible for the postnatal loss of mammalian cardiac regenerative capacity are not fully elucidated. aim present study is to investigate role progesterone in regeneration and explore underlying mechanism.Effect on cardiomyocyte proliferation was analysed by immunofluorescent staining. RNA sequencing performed screen key target genes progesterone, yes-associated protein (YAP) knocked down demonstrate its pro-proliferative effect progesterone. Effect activity YAP promoter...

Several studies have shown that dietary fiber can significantly alter the composition and structure of gut bacterial community in humans mammals. However, few researches been conducted on dynamics assembly across different graded levels regions. To address this, 24 Durco × Bamei crossbred pigs were randomly assigned to four experimental chows comprising fiber. Results showed α-and β-diversity was between cecum jejunum. Adding chow increased α-diversity jejunum cecum, while decreased. The...

Animal gut microbiomes can be clustered into “enterotypes” characterized by an abundance of signature genera. The characteristic determinants, stability, and resilience these community clusters remain poorly understood. We used plateau pika (Ochotona curzoniae) as a model identified three enterotypes 16S rDNA sequencing. Among the top 15 genera, 13 showed significantly different levels between combined with microbial functions distinct fecal short-chain fatty acids. monitored changes in...

Domestication is a key factor of genetic variation; however, the mechanism by which domestication alters gut microbiota poorly understood. Here, to explore variation in structure, function, rapidly evolved genes (REGs), and enzyme profiles cellulase hemicellulose fecal microbiota, we studied wild, half-blood, domestic yaks based on 16S rDNA sequencing, shotgun-metagenomic measurement short-chain-fatty-acids (SCFAs) concentration. Results indicated that wild half-blood harbored an increased...

Assisting in the identification of ergonomic risks for workers: a large vision-language model approach Chao Fan, Qipei Mei, Xinming Li Pages 1010-1017 (2024 Proceedings 41st ISARC, Lille, France, ISBN 978-0-6458322-1-1, ISSN 2413-5844) Abstract: In construction industry, due to workers frequently engaging highly physically demanding tasks and using various tools, are often exposed safety hazards. Various observation-based traditional or computer vision-based artificial intelligence methods...

Plants produce various plant secondary compounds (PSCs) to deter the foraging of herbivorous mammals. However, little is known about whether PSCs can reshape gut microbiota and promote homeostasis hosts. Using 16S rDNA sequencing investigate effects on small mammals, we studied plateau pikas (Ochotona curzoniae) fed diets containing swainsonine (SW) extracted from Oxytropis ochrocephala. Our results showed that both long- short-term treatment a single artificial diet in laboratory...

The browning of white adipose tissue (WAT) is a promising area research for treating metabolic disorders and obesity in the future. However, studies on plant secondary compounds promoting WAT are limited. Herein, we explored effects swainsonine (SW) gut microbiota captive pikas. SW inhibited body mass gain, increased brown (BAT) mass, induced 16S rDNA sequencing revealed significant reduction alpha diversity altered community structure addition to diet significantly relative abundance...

Background: Genetic variants of coding genes related to blood pressure regulation participate in the pathogenesis hypertension and determines response specific antihypertensive drugs. G protein-coupled receptor kinase 4 (GRK4) its are great importance hypertension. However, little is known about role GRK4 determine circadian rhythm candesartan The aim this study was analyze correlation pressure, explore their effect on efficiency candestartan.Methods: In study, a total 1239 cases were...

The gut microbiota plays a crucial role in coronary heart disease (CHD). However, only few studies focusing on the relationship between and CHD ethnic populations are available. Here, we employed shotgun sequencing of metagenome to analyze taxonomic composition functional annotation 14 patients, 13 patients with non-stenosis (NCHD), 18 healthy controls (HT) Tibetan subjects. We found that α-diversity was not significantly different among three groups., whereas β-diversity altered group...

Captivity is an important and efficient technique for rescuing endangered species. However, it induces infertility, the underlying mechanism remains obscure. This study used plateau pika (Ochotona curzoniae) as a model to integrate physiological, metagenomic, metabolomic, transcriptome analyses explore whether dysbiosis of gut microbiota induced by artificial food exacerbates infertility in captive wild animals. Results revealed that captivity significantly decreased testosterone levels...

Abstract The role of miRNAs in the regulation seasonal reproduction rodents, particularly relation to photoperiod changes, is still poorly understood. Previous studies on miRNA transcriptomes striped hamster ( Cricetulus barabensis ) testes have indicated that photoperiodism testes, especially apoptosis, may be influenced by miRNAs. As a functional miRNA, cba‐miR‐222‐3p exhibits suppression under short photoperiod. To elucidate potential testicular hamsters, we exposed male hamsters...

When treating perimembranous ventricular septal defect, median sternotomy leads to unsightly scar, right subaxillary thoracotomy requires cardiopulmonary bypass, and transcatheter device closure constrained by vascular condition. Using small left intercostal incision, we employed transthoracic get around these issues. During this study, was used for surgical repair in 117 patients (Surgical group), whereas incision 131 (Device group). Retrospective data collection analysis were conducted....