A5101514514- Congenital heart defects research
- Advanced Clustering Algorithms Research
- Memory and Neural Mechanisms
- Aortic Disease and Treatment Approaches
- Aortic aneurysm repair treatments
- Congenital Heart Disease Studies
- Complex Systems and Decision Making
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Synthetic Organic Chemistry Methods
- RNA Research and Splicing
- Pulmonary Hypertension Research and Treatments
- Genomic variations and chromosomal abnormalities
- Complex Network Analysis Techniques
- Cancer-related molecular mechanisms research
- Air Quality and Health Impacts
- Cardiomyopathy and Myosin Studies
- Cell Image Analysis Techniques
- Genomics and Rare Diseases
- Cancer Treatment and Pharmacology
- Digital Imaging for Blood Diseases
- Cancer Mechanisms and Therapy
- Chemical Synthesis and Analysis
- Plant Molecular Biology Research
- Inflammation biomarkers and pathways
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2025
Hebei University of Engineering
2025
Affiliated Hospital of Hebei University
2025
Xi'an Jiaotong University
2024
Hangzhou Normal University
2022-2024
Harbin Medical University
2024
Second Affiliated Hospital of Harbin Medical University
2024
Zhejiang University
2024
Fu Wai Hospital
2019-2024
Sichuan University
2024
It is difficult to predict acute thrombotic cardiovascular events in the clinic. Few studies have reported presence of plasma exosomes containing microRNAs (miRNAs) events. Therefore, we aimed investigate levels miR-223, miR-339 and miR-21 before thrombosis mouse models carotid tandem stenosis, as well mechanisms underlying origin function these exosomal miRNAs.Plasma samples were collected from stenosis sham control groups our successfully developed atherothrombosis thrombosis. Platelets...
Cardiac outflow tract (OFT) is a major hotspot for congenital heart diseases. A thorough understanding of the cellular diversity, transitions, and regulatory networks normal OFT development essential to decipher etiology malformations. We performed single-cell transcriptomic sequencing 55,611 mouse cells from three developmental stages that generally correspond early, middle, late remodeling septation. Known such as endothelial-to-mesenchymal transition, have been recapitulated. In...
Abstract Thoracic aortic aneurysm and dissection (TAAD) is a life-threatening condition characterized by medial layer degeneration of the thoracic aorta. A thorough understanding regulator changes during pathogenesis essential for medical therapy development. To delineate cellular molecular development TAAD, we performed single-cell RNA sequencing cells from β-aminopropionitrile-induced TAAD mouse models at three time points that spanned early to advanced stages disease. Comparative analyses...
Hypertrophic cardiomyopathy (HCM) is the most common cardiac genetic disorder characterized by cardiomyocyte hypertrophy and fibrosis. Pathological remodeling in myocardium of HCM patients may progress to heart failure. An in-depth elucidation lineage-specific changes pathological pivotal for development therapies mitigate progression. Here, we performed single-nucleus RNA-seq tissues from or healthy donors conducted spatial transcriptomic assays on tissue sections patients. Unbiased...
Higher plants survive terrestrial water deficiency and fluctuation by arresting cellular activities (dehydration) resuscitating processes (rehydration). However, how monitor availability during rehydration is unknown. Although increases in hypo-osmolarity-induced cytosolic Ca
Viral vectors enable foreign proteins to be expressed in brains of non-genetic species, including non-human primates. However, viruses targeting specific neuron classes have proved elusive. Here we describe viral promoters and strategies for accessing GABAergic interneurons their molecularly defined subsets the rodent primate. Using a set intersection approach, which relies on two co-active promoters, can restrict heterologous protein expression cortical hippocampal somatostatin-positive...
Epidemiological studies have corroborated that respiratory diseases, including lung cancer, are related to fine particulate matter (<2.5 μm) (PM2.5) exposure. The toxic responses of PM2.5 greatly influenced by the source PM2.5. However, effects from Beijing on bronchial genotoxicity scarce. In present study, was sampled and applied in vitro investigate its mechanisms behind it. Human epithelial cells 16HBE were used as a model for Low (67.5 μg/mL), medium (116.9 high (202.5 μg/mL) doses...
Hypertrophic cardiomyopathy (HCM) represents one of the most common heritable heart diseases. However, signalling pathways and regulatory networks underlying pathogenesis HCM remain largely unknown. Here, we present a strand-specific RNA-seq dataset for both coding lncRNA profiling in myocardial tissues from 28 patients 9 healthy donors. This constitutes valuable resource community to examine dysregulated genes versus normal conditions.
An efficient synthesis of quinoxaline-fused aza-bicyclo[2.1.1]hexanes bearing multiple quaternary carbon centers via the intermolecular [2π+2σ] cycloaddition bicyclo[1.1.0]butanes and quinoxalin-2(1H)-ones, facilitated by Lewis acid catalysis, is presented. This reaction carried out under mild conditions exhibits a broad substrate scope excellent functional group tolerance.
Metal-free, photoredox-catalyzed aromatization-driven deconstructive functionalization of spiro-dihydroquinazolinones with α-CF 3 alkenes is achieved high chemoselectivity. Mechanistic studies suggest a radical pathway for this cascade reaction.
Exposure to ambient fine particulate matter (<2.5 μm; PM2.5 ) increases the risk of physiopathology vascular diseases. However, underlying mechanism, particularly mitochondrial damage -induced dysfunction is still unclear. In this study, we examined alterations morphology, and further demonstrated adverse effects on dynamics function in endothelial cells. Consequently, cultured EA.hy926 cells were subjected collected from Beijing. A Cell Counting Assay Kit-8 that exposure decreased...
Triggering receptor expressed on myeloid cells-1 (TREM-1) is a potent amplifier of proinflammatory innate immune reactions, and it an essential mediator death in sepsis. However, the ligand for TREM-1 has not been fully identified. Previous research identified natural distributed platelets that contributed to development exact signal recognization remains be Here, we actin as TREM-1-interacting protein found recombinant could interact with extracellular domain directly. Furthermore,...
Rationale: Transposition of the great arteries (TGA) is one most severe types congenital heart diseases. Understanding clinical characteristics and pathogenesis TGA is, therefore, urgently needed for patient management this disease. However, genetic cause underlying remain largely unexplored. Objective: We sought to systematically examine isolated nonsyndromic TGA. Methods Results: recruited 249 patients with (66 family trios) performed whole-exome sequencing. The incidence patent ductus...
Abstract Aortic root aneurysm is a potentially life‐threatening condition that may lead to aortic rupture and often associated with genetic syndromes, such as Marfan syndrome (MFS). Although studies MFS animal models have provided valuable insights into the pathogenesis of aneurysms, this understanding transcriptomic epigenomic landscape in human tissue remains incomplete. This knowledge gap has impeded development effective targeted therapies. Here, study performs first integrative analysis...
Microcolin B, a potent new immunosuppressant isolated from blue-green alga Lyngbya majuscula off the Venezuelan coast, has been made using methyl-directed asymmetric hydrogenation reaction with rhodium on alumina catalyst lactone 4 for synthesis of key (R,R)-2,4-dimethyloctanoic acid fragment 1. A new, direct mixed imide formation was also developed production unusual prolylpyrrolen-2-one 2 portion microcolin. The pentafluorophenyl ester CBZ-proline 5 reacted lithium imidate lactam 6,...
Resource27 September 2021Open Access Transparent process Single-cell transcriptomic landscape of cardiac neural crest cell derivatives during development Wen Chen orcid.org/0000-0001-6658-1926 State Key Laboratory Cardiovascular Disease, Beijing for Molecular Diagnostics Diseases, Center Medicine, Fuwai Hospital, National Chinese Academy Medical Sciences and Peking Union College, Beijing, China Search more papers by this author Xuanyu Liu Corresponding Author [email protected]...
Genetic mutants defective in stimulus-induced Ca2+ increases have been gradually isolated, allowing the identification of cell-surface sensors/receptors, such as osmosensor OSCA1. However, determining -signaling specificity to various stimuli these remains a challenge. For instance, less is known about exact selectivity between osmotic and ionic stresses osca1 mutant. Here, we developed method distinguish effects by analyzing increases, demonstrated that impaired primarily induced but not...
Investigators conducting behavioral experiments often need precise control over the timing of delivery stimuli to subjects and collect times subsequent responses. Furthermore, investigators want fine-tuned how various multi-modal cues are presented. behaviorMate takes an “Intranet Things” approach, using a networked system hardware software components for achieving these goals. The outputs file with integrated timestamp-event pairs that can then format process their own analysis pipelines....
Investigators conducting behavioral experiments often need precise control over the timing of delivery stimuli to subjects and collect times subsequent responses. Furthermore, investigators want fine-tuned how various multi-modal cues are presented. behaviorMate takes an ‘Intranet Things’ approach, using a networked system hardware software components for achieving these goals. The outputs file with integrated timestamp–event pairs that can then format process their own analysis pipelines....