A5102875694- interferon and immune responses
- Influenza Virus Research Studies
- Streptococcal Infections and Treatments
- Immune Response and Inflammation
- Microbial infections and disease research
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Neonatal and Maternal Infections
- COVID-19 Clinical Research Studies
- Viral Infections and Immunology Research
- Animal Disease Management and Epidemiology
- RNA modifications and cancer
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Respiratory viral infections research
- Long-Term Effects of COVID-19
- Viral Infectious Diseases and Gene Expression in Insects
- Cytokine Signaling Pathways and Interactions
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Vector-Borne Animal Diseases
- RNA and protein synthesis mechanisms
- Probiotics and Fermented Foods
- Gut microbiota and health
- Autophagy in Disease and Therapy
Huazhong Agricultural University
2016-2025
Center of Hubei Cooperative Innovation for Emissions Trading System
2016-2024
Yanbian University Hospital
2023
State Key Laboratory of Agricultural Microbiology
2022
Shenzhen Institutes of Advanced Technology
2021
Chinese Academy of Sciences
2021
Ministry of Agriculture and Rural Affairs
2019
Hunan University
2018
Changsha University
2018
Chinese University of Hong Kong
2004
COVID-19 is a new respiratory illness caused by SARS-CoV-2, and has constituted global public health emergency. Cat susceptible to SARS-CoV-2. However, the prevalence of SARS-CoV-2 in cats remains largely unknown. Here, we investigated infection during outbreak Wuhan serological detection methods. A cohort serum samples were collected from Wuhan, including 102 sampled after outbreak, 39 prior outbreak. Fifteen sera positive for receptor binding domain (RBD) indirect enzyme linked...
Summary Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in Wuhan, China, and rapidly spread worldwide. Previous studies suggested cat could be a potential susceptible animal of SARS-CoV-2. Here, we investigated the infection SARS-CoV-2 cats detecting specific serum antibodies. A cohort samples were collected from including 102 sampled after COVID-19 outbreak, 39 prior to outbreak. 15 (14.7%) sera outbreak positive...
Influenza A virus (IAV) infection induces mitophagy, which is essential for the clearance of damaged mitochondria. Dysfunctional mitochondria can be selectively targeted by PINK1, recruits PRKN/PARK2 and leads to subsequent mitochondrial sequestration within autophagosomes. The IAV PB1-F2 protein translocates mitochondria, accelerates fragmentation impairs innate immunity. However, whether mediates IAV–induced mitophagy relation between PB1-F2-attenuated immunity remain obscure. Here, we...
BackgroundAn ideal animal model to study SARS-coronavirus 2 (SARS-CoV-2) pathogenesis and evaluate therapies vaccines should reproduce SARS-CoV-2 infection recapitulate lung disease like those seen in humans. The angiotensin-converting enzyme (ACE2) is a functional receptor for SARS-CoV-2, but mice are resistant the because their ACE2 incompatible with receptor-binding domain (RBD) of spike protein .MethodsSARS-CoV-2 was passaged BALB/c obtain mouse-adapted virus strain. Complete genome deep...
The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and high mortality rate in patients. However, there very limited information on effect infection integrity blood-brain barrier (BBB).RNA-sequencing profiling was performed to analyze transcriptomic changes human brain microvascular endothelial cells (hBMECs) after infection. Bioinformatic tools were used for differential...
Abstract Background Influenza is a severe respiratory illness that continually threatens global health. It has been widely known gut microbiota modulates the host response to protect against influenza infection, but mechanistic details remain largely unknown. Here, we took advantage of phenomenon lethal dose 50 (LD ) and metagenomic sequencing analysis identify specific anti-influenza microbes analyze underlying mechanism. Results Transferring fecal from mice survive virulent H7N9 infection...
Infection with the Streptococcus suis (S. suis) epidemic strain can cause Streptococcal toxic shock-like syndrome (STSLS), which is characterized by a cytokine storm, dysfunction of multiple organs and high incidence mortality despite adequate treatment. Despite some progress concerning contribution inflammatory response to STSLS, precise mechanism underlying STSLS development remains elusive. Here, we use murine model demonstrate that caspase-1 activity critical for development....
Influenza A virus can evade host innate immune response that is involved in several viral proteins with complicated mechanisms. To date, how influenza M2 protein modulates the immunity remains unclear. Herein, we showed colocalized and interacted MAVS (mitochondrial antiviral signaling protein) on mitochondria, positively regulated MAVS-mediated immunity. Further studies revealed induced reactive oxygen species (ROS) production was required for activation of macroautophagy/autophagy...
Highly pathogenic H5N1 infections are often accompanied by excessive pro-inflammatory response, high viral titer, and apoptosis; as such, the efficient control of these poses a great challenge. The pathogenesis influenza virus infection is also related to oxidative stress. However, role endogenic genes with antioxidant effect in viruses, especially should be further investigated. In this study, lung epithelial cells decreased Cu/Zn superoxide dismutase (SOD1) expression at mRNA protein...
H5N1 influenza A virus (IAV) causes severe respiratory diseases and high mortality rates in animals humans. MicroRNAs are being increasingly studied to evaluate their potential as therapeutic entities combat viral infection. However, mechanistic studies delineating the roles of microRNAs regulating host-H5N1 interactions remain scarce. Here, we performed microRNA microarray analysis using A549 human lung epithelial cells infected with a highly pathogenic avian virus. The expression profile...
Argonaute proteins are present and conserved in all domains of life. Recently characterized prokaryotic (pAgos) participates host defense by DNA interference. Here, we report that the Natronobacterium gregoryi (NgAgo) enhances gene insertions or deletions Pasteurella multocida Escherichia coli at efficiencies 80–100%. Additionally, effects a homologous arms-dependent but guide DNA- potential enzyme activity-independent manner. Interestingly, such were also observed other pAgos fragments...
Age is a risk factor for coronavirus disease 2019 (COVID-19) associated morbidity and mortality in humans; hence, this study, we compared the course of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) infection young aged BALB/c mice. We found that SARS-CoV-2 isolates replicated tracts 12-month-old (aged) mice caused pathological features pneumonia upon intranasal infection. In contrast, rapid viral clearance was observed 5 days following 2-month-old (young) with no evidence...
Influenza virus infection can alter the composition of gut microbiota, while its pathogenicity can, in turn, be highly influenced by microbiota. However, details underlying these associations remain to determined. The H7N9 influenza is an emerging zoonotic pathogen which has caused death 616 humans and incurred huge losses poultry industry. Here, we investigated effects with pathogenic on microbiota determined potential anti-influenza microbes. 16S rRNA sequencing results show that alters...
The continuous spread of highly pathogenic avian influenza virus (AIV) subtype H5N1 is threatening the poultry industry and human health worldwide. Rapid sensitive diagnostic methods are required for surveillance. In this study, fluorescent (FL) probe CdTe quantum dots (QDs) was designed using covalently linked rabbit anti-AIV antibody. Based on these QD-antibody conjugates, a novel sandwich FL-linked immunosorbent assay (sFLISA) developed viral antigen detection. sFLISA allowed...
Western and indigenous Chinese pig breeds show obvious differences in muscle growth meat quality; however, the underlying molecular mechanism remains unclear. In this study, proteome analysis of LM between purebred Meishan Large White pigs was performed by 2-dimensional gel electrophoresis mass spectrometry. A total 25 protein spots were differentially expressed 2 breeds. The 14 identified proteins could be divided into 4 groups: energy metabolism, defense stress, myofibrillar filaments,...
Duck enteritis virus (DEV), duck tembusu (DTMUV), and highly pathogenic avian influenza (HPAIV) H5N1 are the most important viral pathogens in ducks, as they cause significant economic losses industry. Development of a novel vaccine simultaneously effective against these three viruses is economical method for reducing losses. In present study, by utilizing clustered regularly interspaced short palindromic repeats (CRISPR)/associated 9 (Cas9)-mediated gene editing strategy, we efficiently...
The novel coronavirus disease (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has resulted in an unprecedented public health crisis and economic losses. Although several cases of cats dogs infected with SARS-CoV-2 have been reported during this outbreak, the prevalence dog its transmission among other companion animals are still unknown. Here, we report extensive serological study infection Wuhan analyse rates at different stages pandemic outbreak. A total 946 serum...
N6-methyladenosine (m6A) modification on viral RNAs has a profound impact infectivity. m6A is also highly pervasive for influenza RNAs. However, its role in virus mRNA splicing largely unknown. Here, we identify the reader protein YTHDC1 as host factor that associates with A NS1 and modulates splicing. levels are enhanced by IAV infection. We demonstrate inhibits NS binding to an 3' site promotes replication pathogenicity vitro vivo. Our results provide mechanistic understanding of IAV-host...
Triggering receptor expressed on myeloid cells-1 (TREM-1) is a potent amplifier of proinflammatory innate immune reactions, and it an essential mediator death in sepsis. However, the ligand for TREM-1 has not been fully identified. Previous research identified natural distributed platelets that contributed to development exact signal recognization remains be Here, we actin as TREM-1-interacting protein found recombinant could interact with extracellular domain directly. Furthermore,...
The influenza A virus poses serious public health challenges worldwide. Strikingly, small noncoding microRNAs (miRNAs) that modulate gene expression are closely involved in antiviral responses, although the underlying mechanisms essentially unknown. We now report microRNA-340 (miR340) is downregulated following and other RNA infections, implying host cells deplete miR340 as an defense mechanism. Accordingly, inhibition or knockdown of endogenous clearly prevents infection cultured cells,...