A5034570457- Reproductive Biology and Fertility
- Platelet Disorders and Treatments
- Pregnancy and preeclampsia studies
- Assisted Reproductive Technology and Twin Pregnancy
- Sperm and Testicular Function
- Maternal and Perinatal Health Interventions
- Ovarian function and disorders
- Prenatal Screening and Diagnostics
- Gastric Cancer Management and Outcomes
- Maternal and fetal healthcare
- Renal and related cancers
- Blood Coagulation and Thrombosis Mechanisms
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Total Knee Arthroplasty Outcomes
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pregnancy-related medical research
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Pluripotent Stem Cells Research
- HVDC Systems and Fault Protection
- Epigenetics and DNA Methylation
- Neonatal Respiratory Health Research
- Lower Extremity Biomechanics and Pathologies
- Optical Network Technologies
Reproductive & Genetic Hospital CITIC-Xiangya
2017-2025
Chinese Academy of Agricultural Sciences
2023-2025
Soochow University
1992-2025
Oil Crops Research Institute
2023-2025
Chongqing University
2025
Zhongnan Hospital of Wuhan University
2014-2024
Wuhan University
2014-2024
Ruijin Hospital
2013-2024
Tianjin Medical University
2023-2024
Central South University
2017-2024
Our laboratory has reported that two major noncollagenous dentin proteins, sialoprotein and phosphoprotein, are specific cleavage products of a larger precursor protein termed sialophosphoprotein (MacDougall, M., Simmons, D., Luan, X., Nydegger, J., Feng, J. Q., Gu, T. (1997) <i>J. Biol. Chem.</i> 272:835–842). To confirm our single gene hypothesis initiate <i>in vitro</i>promoter studies, we have characterized the structural organization mouse gene. This transcription unit ∼9.4 kilobase...
Abstract Because of the absence methods for tracking RNA G‐quadruplex dynamics, especially folding and unfolding this attractive structure in live cells, understanding biological roles G‐quadruplexes is so far limited. Herein, we report a new red‐emitting fluorescent probe, QUMA‐1 , selective, continuous, real‐time visualization cells. The applications several previously intractable applications, including live‐cell imaging dynamic folding, unfolding, movement unwinding by helicase have been...
The arginine/glycine-rich region termed the RGG domain is usually found in G-quadruplex (G4)-binding proteins and important G4–protein interactions. Studies on binding mechanism of domains that small segments (RGG motif) inside contribute greatly to G4 affinity. However, unlike entire have been broadly explored, role motif remains obscure, with very limited study. Herein, clarify interactions, we systematically investigated affinity mode between RGG-motif peptides G4s. internal arrangement...
The mitochondrial DNA G-quadruplex (mtDNA G4) is a potential regulatory element for the regulation of functions; however, its relevance and specific roles in diseases remain largely unknown. Here, we engineered set chemical probes, including MitoISCH, an mtDNA G4-specific fluorescent probe, together with MitoPDS, mitochondria-targeted G4-stabilizing agent, to thoroughly investigate G4s. Using MitoISCH monitor previously intractable dynamics G4s, surprisingly found that their formation was...
PROteolysis TArgeting Chimeras (PROTACs) induce targeted protein degradation by hijacking the intracellular ubiquitin proteasome system, thus emerging as a new strategy for drug development. However, most PROTACs generated lack cell-type selectivity and are poorly soluble in water. To address this drawback, we developed novel PROTAC ZL216 using aptamer AS1411 targeting ligand of nucleolin to conjugate with small molecule E3 ligase VHL, which shows high aqueous solubility serum stability....
Homocysteine (Hcy) is an independent risk factor for cardiovascular disease. Monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) are major chemokines leukocyte trafficking have been identified in atheromatous plaques. MCP-1 IL-8 found to express mainly by macrophages human lesion. We undertook this study determine whether Hcy could induce the secretion of from monocytes and, if so, explore mediating mechanism. that clinically relevant levels (10 1000 micromol/L) increased...
Does single cleavage-stage (Day 3) embryo transfer using a time-lapse (TL) hierarchical classification model achieve comparable ongoing pregnancy rates (OPR) to blastocyst 5) by conventional morphological (CM) selection? Day 3 (SET) with had significantly lower OPR compared 5 SET CM selection. Cleavage-stage is an alternative SET. Time-lapse imaging assists better selection, based on studies of outcomes when adding selection at the cleavage or stage. This single-centre, randomized,...
Total fertilization failure (TFF), which is the of in all oocytes, occurs 1%-3% intracytoplasmic sperm injection (ICSI) cycles. However, sperm-related factors that cause humans are still largely unknown. Here, we identified three novel homozygous variations PLCZ1 gene a recessive inheritance pattern consanguineous families, located key catalytic domain, and predicted to modify its secondary structure thus impair hydrolytic activity. Moreover, immunofluorescent staining revealed PLCζ mutant...
The presence of antiphospholipid antibodies was shown to be associated with thrombosis in coronavirus disease 2019 (COVID-19) patients. Recently, according reports from several studies, the vaccine-induced immune thrombotic thrombocytopenia is mediated by anti-platelet factor 4 (PF4)-polyanion complex adenovirus-vectored COVID-19 vaccine recipients. It impendent explore whether inactivated widely used China influences prothrombotic autoantibody production and induces thrombosis. In this...
In the context of continuous technological progress, application information technology in field education has gradually opened door to innovation. The integration and curriculum is use present a new way teaching process. Therefore, compared with traditional mode, more flexible can show boring classroom vividly graphically front students, thus motivating students learn actively improving efficiency. recent years, development computer technology, rise such as data analysis Internet Things...
Maternal messenger ribonucleic acids (mRNAs) are driven by a highly orchestrated scheme of recruitment to polysomes and translational activation. However, selecting regulating individual mRNAs for the translation from competitive pool little-known processes. This research shows that maternal eukaryotic initiation factor 4e1b (Eif4e1b) expresses during oocyte-to-embryo transition (OET), deletion Eif4e1b leads multiple defects concerning oogenesis embryonic developmental competence OET. The...