A5021220046- dental development and anomalies
- Bone and Dental Protein Studies
- Oral and Maxillofacial Pathology
- Cleft Lip and Palate Research
- Oral microbiology and periodontitis research
- Cancer-related gene regulation
- Health and Medical Research Impacts
- Endodontics and Root Canal Treatments
- Bone Metabolism and Diseases
- Dental Education, Practice, Research
- Craniofacial Disorders and Treatments
- Dental Health and Care Utilization
- Supramolecular Self-Assembly in Materials
- Fibroblast Growth Factor Research
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Dental Research and COVID-19
- Hedgehog Signaling Pathway Studies
- Proteoglycans and glycosaminoglycans research
- Bone Tissue Engineering Materials
- Diversity and Career in Medicine
- Dental materials and restorations
- Connective tissue disorders research
- RNA Interference and Gene Delivery
- Innovations in Medical Education
National Institutes of Health
2021-2025
Kyoto University
2025
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2023-2024
National Institute of Dental and Craniofacial Research
2021-2024
United States Department of Health and Human Services
2023
Office of the Director
2021
University of Utah
2013-2020
International Association for Dental Research
2013-2019
Texas A&M University
2007-2017
Baylor University
2006-2017
Dentin sialophosphoprotein (Dspp) is mainly expressed in teeth by the odontoblasts and preameloblasts. The Dspp mRNA translated into a single protein, Dspp, cleaved two peptides, dentin sialoprotein phosphoprotein, that are localized within matrix. Recently, mutations this gene were identified human dentinogenesis imperfecta II (Online Mendelian Inheritance Man (OMIM) accession number 125490) dysplasia (OMIM 125420) syndromes. Herein, we report generation of Dspp-null mice develop tooth...
Multidomain peptides are a class of amphiphilic self-assembling with modular ABA block motif in which the B drives self-assembly while flanking A blocks, electrostatically charged, control conditions under assembly takes place. Previously we have shown that careful selection amino acids and blocks allow one to self-assembled fiber length viscoelastic properties formed hydrogels. Here demonstrate how nature this peptide assembler can be designed for biological applications. With over...
Root canal therapy is common practice in dentistry. During this procedure, the inflamed or necrotic dental pulp removed and replaced with a synthetic material. However, recent research provides evidence that engineering of dentin possible by using biologically driven approaches. As tissue strategies hold promise to soon supersede conventional root treatment, there need for customized scaffolds stem cell delivery recruitment. We hypothesize incorporation pulp-derived cells bioactive factors...
Abstract Although the precise mechanisms of conversion predentin to dentin are not well understood, several lines evidence implicate noncollagenous proteins (NCPs) as important regulators biomineralization. Here we compared in vivo temporospatial expression patterns two NCP genes, matrix protein 1 (Dmp1), and sialophosphoprotein (DSPP) developing molars. Reverse transcription-polymerase chain reaction was performed on embryonic day 13 1-day-old first molars using Dmp1- DSPP-specific primer...
ABSTRACT Osteoblasts and odontoblasts, cells that are responsible for the formation of bone dentin matrices respectively, share several molecular characteristics. Recently, Cbfa1 was shown to be a critical transcriptional regulator osteoblast differentiation. Mutations in this gene cause cleidocranial dysplasia (CCD), an autosomal dominant disorder humans mice characterized by defective formation. CCD also results dental defects include supernumerary teeth delayed eruption permanent...
Our laboratory has reported that two major noncollagenous dentin proteins, sialoprotein and phosphoprotein, are specific cleavage products of a larger precursor protein termed sialophosphoprotein (MacDougall, M., Simmons, D., Luan, X., Nydegger, J., Feng, J. Q., Gu, T. (1997) <i>J. Biol. Chem.</i> 272:835–842). To confirm our single gene hypothesis initiate <i>in vitro</i>promoter studies, we have characterized the structural organization mouse gene. This transcription unit ∼9.4 kilobase...
Dental caries remains one of the most prevalent infectious diseases in world. So far, available treatment methods rely on replacement decayed soft and mineralized tissue with inert biomaterials alone. As an approach to develop novel regenerative strategies engineer dental tissues, two stem cell lines were combined peptide-amphiphile (PA) hydrogel scaffolds. PAs self-assemble into three-dimensional networks nanofibers, living cells can be encapsulated. Cell-matrix interactions tailored by...
Postnatal stem cells can generate tooth-specific structures after transplantation in vivo, which makes them a valuable tool for dental tissue engineering. Scaffold materials that are compatible with cells, injectable and tunable targeted regeneration needed. A candidate material is fibrin, biopolymer critical to hemostasis wound healing. Rapid degradation of fibrin be decelerated by modification polyethylene glycol (PEG), thus creating hybrid cell delivery. The aim this study was evaluate...
Abstract To study the fate of bone cells, we used transferase-mediated, biotin-dUTP nick end-labeling (TUNEL) assay to detect DNA fragmentation during formation intramembranous and endochondral in newly born hamsters, mice, rats. In alveolar forming around developing tooth crowns, was found three cell types: TRAP-negative mononuclear cells at surface, osteocytes, some but not all nuclei TRAP-positive osteoclasts. Osteoblasts did undergo fragmentation. A strong positive correlation between...
Matrix Gla protein (MGP) is, along with osteocalcin, a skeletal member of the family extracellular mineral-binding proteins. Although precise function these proteins remains obscure, circumstantial evidence suggests that they play role in endochondral ossification. As first step toward understanding MGP we have performed preliminary characterization its promoter element and studied developmental pattern expression this gene. DNA transfection experiments indicate mouse functions better cells...
Genetic and molecular studies in humans mice indicate that Runx2 (Cbfa1) is a critical transcriptional regulator of bone tooth formation. Heterozygous mutations cause cleidocranial dysplasia (CCD), an inherited disorder characterized by skeletal defects, supernumerary teeth, delayed eruption. Mice lacking the gene die at birth lack development. Our extended phenotypic mutants showed developing teeth fail to advance beyond bud stage mandibular molar organs were more severely affected than...
Pax9 and Msx1 encode transcription factors that are known to be essential for the switch in odontogenic potential from epithelium mesenchyme. Multiple lines of evidence suggest these molecules play an important role maintenance mesenchymal Bmp4 expression, which ultimately drives morphogenesis dental organ. Here we demonstrate is able directly regulate expression interact with at protein level enhance its ability transactivate during tooth development. In addition, tested how a missense...