We have investigated the mechanism by which osteoclasts adhere to and resorb bone.We show that these cells express B1 B3 integrins are involved in attachment purified bone matrix proteins.Binding osteopontin sialoprotein is mediated a,.&, while a integrin responsible for fibronectin.Both rapid intact particles their subsequent resorption blocked monoclonal antibody directed a& complex but not an against 81 integrins.Attachment of also inhibited with soluble osteopontin,...

A phosphorylated glycoprotein was purified from the mixture of proteins extracted by demineralization rat bone with 0.5 M EDTA in 4 guanidinium chloride. high level purity for preparation indicated a single band on sodium dodecyl sulfate (SDS)-gradient gel electrophoresis, sedimentation equilibrium ultracentrifugal data, and automated Edman degradation results. The molecular weight phosphoprotein shown to be about 44,000 analyses chloride, even though an Mr 75,000 obtained 5-15%...

Article1 August 1964Nephrotoxicity of Amphotericin BEarly and Late Effects in 81 PatientsWILLIAM T. BUTLER, M.D., JOHN E. BENNETT, DAVID W. ALLING, PH.D., PAUL WERTLAKE, P. UTZ, GEORGE J. HILL II, M.D.WILLIAM M.D.Author, Article, Disclosure Informationhttps://doi.org/10.7326/0003-4819-61-2-175 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptAmphotericin B is the only available drug effective preventing death...

To study the effects of methylprednisolone on immune mechanisms in absence other immunosuppressive agents or immunologically mediated diseases, we gave 17 normal adult male volunteers 96 mg daily for 3-5 days and compared results with 12 untreated controls who were studied simultaneously, 86% treated had significant decreases concentrations serum IgG. 2-4 wk after methylprednisolone, a mean decrease IgG 22% only 1% controls. Likewise, IgA concentration occurred 43% volunteers, whereas IgM...

Abstract Soluble collagen from guinea pig skin has been subjected to sequential digestion with purified collagenase and trypsin. The small quantity of hexose present in the can be detected digest largely as relatively basic glycopeptides. One these glycopeptides obtained pure form accounts for at least 30% original collagen. This glycopeptide shown consist a disaccharide glucose galactose linked O-glycosidically hydroxyl group hydroxylysine residue special peptide region molecule. amino acid...

Dentin sialophosphoprotein [designated DSPP and cleaved into dentin sialoprotein (DSP) phosphoprotein (DPP)], enamelysin ameloblastin are each expressed in unique fashions during tooth development. It is possible that these components participate cell differentiation the conversion of unmineralized matrix mineralized structures. In order to delineate timing positioning three molecules a physiological context, we compared their expression profiles by performing situ hybridization experiments...

Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP) are expressed as a single mRNA transcript coding for large precursor protein termed sialophosphoprotein (DSPP). DSP, DPP, DSPP have been considered to be tooth-specific. To test the expression of dspp gene in bone, we performed Western immunoblots reverse-transcription polymerase chain-reaction (RT-PCR). With immunoblots, detected DSP Gdm/EDTA extracts rat long at level about 1/400 that dentin. Using RT-PCR, mouse calvaria. Similar...

Unique polypeptide chains have been isolated from S-sulfonated light-chain fractions of human serum immunoglobulin M and colostral A. Their electrophoretic mobilities, molecular weights, peptide maps, amino acid compositions, antigenic determinants are very similar or perhaps identical but differ those light secretory piece.

Abstract The nature and tissue distribution of non‐collagenous bone proteins synthesized by adult rat marrow cells, induced to differentiate in the presence dexamethasone (DEX) β‐glycerophosphate (β‐GP), was studied vitro determine potential role these formation. Northern hybridization analysis revealed a strong induction sialoprotein (BSP) osteocalcin DEX‐treated cultures, whereas constitutive expression secreted phosphoprotein I (SPP‐1), type collagen, SPARC, alkaline phos‐phatase...

Type beta transforming growth factor (TGF beta) was shown to regulate the production of several extracellular matrix proteins. Osteopontin (OP) is a recently discovered bone protein which promote attachment osteoblastic rat osteosarcoma ROS 17/2.8 cells their substrate. We examined effects TGF on OP and mRNA in cells. Four-day treatment with 4 ng/ml 1 increased substantially level osteopontin cell culture media, as estimated by immunoblotting. Metabolic labeling showed that this effect...

Noncollagenous proteins (NCPs) were obtained from rat dentin using several precautionary measures to prevent artifactual degradation and losses of the proteins.Prior demineralization, incisor was extracted with 4 M guanidine hydrochloride (GdmCl) containing enzyme inhibitors.The only major component GdmCl a proteoglycan fraction.Most NCPs when incisors decalcified an EDTA solution protease extract contained four types macromolecules: acidic glycoproteins, y-carboxyglutamic acid...

BEFORE the introduction of amphotericin B in 1956 cryptococcal meningitis was almost always fatal, three quarters patients dying during first year illness.1 2 3 4 5 6 7 8 9 10 11 Occasionally, progression disease interrupted by temporary remissions12; nevertheless, survival for longer than years unusual. Since has come into use, however, improvement and recovery have been described a number cases.13 14 15 16 17 18 19 20 21 22 In present report 40 cases seen at National Institutes Health will...

The high-resolution, postembedding protein A-gold immunocytochemical technique was used to visualize the distribution of two noncollagenous bone proteins, osteopontin (OPN) and osteocalcin (OC), plasma alpha 2HS-glycoprotein (alpha 2HS-GP) albumin (ALB), in sections Lowicryl K4M-embedded rat tibial alveolar bone. In primary spongiosa metaphysis, a seam organic material (lamina limitans) that labeled intensely with OPN OC antibodies observed at bone/calcified cartilage interface just below...

Polyclonal antibodies against a 44-KD phosphoprotein (44K BPP) from rat bone were raised in rabbits, affinity-purified, and used as probes to study the protein's distribution various types of developing bones newborn rats. Three immunostaining procedures applied utilizing indirect immunofluorescence, avidin-biotin-peroxidase complex, avidin-gold complex with silver enhancement. All methods gave essentially identical and/or complementary results. Antigenicity for anti-44K BPP was detected...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIsolation and Characterization of the Cyanogen Bromide Peptides from α1 Chain Rat Skin Collagen*William T. Butler, Karl A. Piez, Paul BornsteinCite this: Biochemistry 1967, 6, 12, 3771–3780Publication Date (Print):December 1, 1967Publication History Published online1 May 2002Published inissue 1 December 1967https://pubs.acs.org/doi/10.1021/bi00864a022https://doi.org/10.1021/bi00864a022research-articleACS PublicationsRequest reuse permissionsArticle...

Dentin matrix protein-1 (DMP1) is a mineralized tissue protein synthesized by osteoblasts, hypertrophic chondrocytes, and ameloblasts as well odontoblasts. DMP1 believed to have multiple in vivo functions, acting both signaling molecule regulator of biomineralization. Using cell-free system vitro, we evaluated the action regulation hydroxylapatite (HA) formation crystal growth. The non-phosphorylated recombinant acted an HA nucleator, increasing amount mineral formed gelatin gel growth...