A5038500890- Lipid Membrane Structure and Behavior
- PI3K/AKT/mTOR signaling in cancer
- Cellular transport and secretion
- Spectroscopy and Quantum Chemical Studies
- Protein Kinase Regulation and GTPase Signaling
- Erythrocyte Function and Pathophysiology
- Protein Structure and Dynamics
- Sphingolipid Metabolism and Signaling
- Surfactants and Colloidal Systems
- Analytical Chemistry and Chromatography
- Electrochemical Analysis and Applications
- Microtubule and mitosis dynamics
- Lipid metabolism and biosynthesis
- Cancer, Lipids, and Metabolism
- RNA and protein synthesis mechanisms
- Bone and Dental Protein Studies
- Advanced Chemical Sensor Technologies
- Mast cells and histamine
- Mass Spectrometry Techniques and Applications
- Spectroscopy and Chemometric Analyses
- Cellular Mechanics and Interactions
- Advanced biosensing and bioanalysis techniques
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Spectroscopy Techniques in Biomedical and Chemical Research
- RNA Interference and Gene Delivery
Worcester Polytechnic Institute
2014-2024
Kent State University
2004-2022
Texas A&M University System
2010
Hospital for Special Surgery
2010
University of Massachusetts Chan Medical School
2008
Rutgers, The State University of New Jersey
1995-2003
University of Alberta
2003
Universität Hamburg
1992-2002
Martin Luther University Halle-Wittenberg
1989-1999
The theory and practice of external infrared reflection absorption spectrometry (IRRAS) as applied to monomolecular films at the air-water interface are reviewed. observed IR frequencies for amphiphilic species provide information about conformational states hydrocarbon chains hydrogen bonding ionization polar head groups, under conditions controlled surface pressure. Determination molecular orientation is also feasible requires detailed consideration reflection-absorption properties three...
Monolayers of behenic acid methyl ester at the air/H2O and air/D2O interfaces provide a convenient test for quantitative analysis infrared reflection−absorption spectroscopy (IRRAS) intensities. Spectra were acquired both s- p-polarized radiation angles incidence 35°, 40°, 45°, 50°. The observed ∼10 cm-1 splitting (at surface pressure 14 mN/m) methylene scissoring rocking modes provides direct evidence occurrence perpendicular orthorhombic subcell structure existence all-trans acyl chains....
Phosphatidylinositol polyphosphate lipids (phosphoinositides) form only a minor pool of membrane phospholipids but are involved in many intracellular signaling processes, including trafficking, cytoskeletal remodeling, and receptor signal transduction. Phosphoinositide properties largely determined by the characteristics their headgroup, which at physiological pH is highly charged also capable forming hydrogen bonds. Many proteins have developed special binding domains that facilitate...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIn situ investigation of saturated long-chain fatty acids at the air/water interface by external infrared reflection-absorption spectrometryArne Gericke and Heinrich HuehnerfussCite this: J. Phys. Chem. 1993, 97, 49, 12899–12908Publication Date (Print):December 1, 1993Publication History Published online1 May 2002Published inissue 1 December 1993https://pubs.acs.org/doi/10.1021/j100151a044https://doi.org/10.1021/j100151a044research-articleACS...
Infrared reflection−absorption spectroscopic (IRRAS) measurements of octadecanoic acid monolayers on aqueous subphases containing 1 mM BaCl2, CuCl2, NiCl2, and ZnCl2, respectively, supplied insight into the molecular order coordination complexes system alkanoic acid/bivalent cation. The formation highly ordered crystalline domains already at large areas per molecule is reflected by antisymmetric methylene stretching vibrations which are independent compression status, however,...
Dentin matrix protein-1 (DMP1) is a major synthetic product of hypertrophic chondrocytes and osteocytes. Previous in vitro studies showed full-length DMP1 inhibits hydroxyapatite (HA) formation growth, while its N-terminal fragment (37K) promotes HA formation. Since there are 3 fragments within the mineralized tissues [N-terminal, C-terminal (57K), chondroitin-sulfate-linked (DMP1-PG)], we predicted that each would have distinct effect on mineralization related to interaction with HA. In...
All-atom molecular dynamics simulations combined with graph-theoretic analysis reveal that clustering of monomethyl phosphate dianion (MMP2-) is strongly influenced by the types and combinations cations in aqueous solution. Although Ca2+ promotes formation stable large MMP2- clusters, K+ alone does not. Nonetheless, clusters are larger their link lifetimes longer mixtures Ca2+. This "synergistic" effect depends sensitively on Lennard-Jones interaction parameters between phosphorus oxygen...
PTEN (phosphatase and tensin homologue deleted on chromosome 10) is a tumor suppressor that mutated or in variety of human tumors, even loss only one gene profoundly affects carcinogenesis. encodes phosphatidylinositol phosphate phosphatase specific for the 3-position inositol ring. Despite its importance, we are just beginning to understand regulatory circuits maintain correct levels activity. Several independent studies reported PI(4,5)P2 enhances activity, but reasons this enhancement...
The structure and function of the PTEN phosphatase is investigated by studying its membrane affinity localization on in-plane fluid, thermally disordered synthetic models. association protein depends strongly composition, where phosphatidylserine (PS) phosphatidylinositol diphosphate (PI(4,5)P2) act pronouncedly synergistic in pulling enzyme to surface. equilibrium dissociation constants for binding wild type (wt) PS PI(4,5)P2 were determined be Kd∼12 µM 0.4 µM, respectively, Kd∼50 nM if...
With the discovery of an isoform based on alternative translation start site, PTEN nomenclature needs update.