Dentin sialophosphoprotein (Dspp) is mainly expressed in teeth by the odontoblasts and preameloblasts. The Dspp mRNA translated into a single protein, Dspp, cleaved two peptides, dentin sialoprotein phosphoprotein, that are localized within matrix. Recently, mutations this gene were identified human dentinogenesis imperfecta II (Online Mendelian Inheritance Man (OMIM) accession number 125490) dysplasia (OMIM 125420) syndromes. Herein, we report generation of Dspp-null mice develop tooth...

Abstract Background HMGA2 expression has been shown to be associated with enhanced selective chemosensitivity towards the topoisomerase (topo) II inhibitor, doxorubicin, in cancer cells. Although roles of signaling cascades and proteins as regulatory factors development, neoplasia adaptation environment are becoming well established, evidence for involvement small RNA molecules, such microRNAs (miRNAs) important regulators both transcriptional posttranscriptional gene silencing is presently...

The GTPase-activating protein (GAP) stimulates the GTPase reaction of p21 by 5 orders magnitude such that kcat is increased to 19 s-1. Mutations residues in loop L1 (Gly-12 and Gly-13), L2 (Thr-35 Asp-38), L4 (Gln-61 Glu-63) influence different ways, but all these mutant proteins still form complexes with GAP. C-terminal domain human GAP gene product, GAP334, which comprises 714 1047, 20 times less active than full-length on a molar basis has fourfold lower affinity. This finding indicates N...

Abstract Emerging knowledge on how the dysregulated function of signaling networks contributes to malignant growth squamous cell carcinoma head and neck (HNSCC) can now be exploited identify novel mechanism-based anticancer treatments. In this regard, we have observed that persistent activation serine/threonine kinase Akt is a frequent event in HNSCC, blockade its upstream kinase, 3′-phosphoinositide-dependent 1, potently inhibits tumor growth. promotes proliferation by ability coordinate...

A primary objective in the treatment of periodontal diseases is regeneration mineralized and soft connective tissue components attachment apparatus. Current theories suggest cells periodontium have capacity, when appropriately triggered, to actively participate restoring tissues, including tissues. To evaluate further role such homeostasis, ligament (PDL) gingival fibroblasts (GF) were cultured examined for alkaline phosphatase levels ability produce nodules culture. These are two...

Squamous carcinomas of the head and neck (HNSCC) represent sixth most common cancer among men worldwide a major cause morbidity mortality due to its relatively poor prognosis. As part ongoing studies addressing molecular events underlying tumor progression in HNSCC, we have explored nature proliferative pathways which dysregulation may promote aberrant cell growth this type. The serine/threonine protein kinase Akt is downstream target phosphatidylinositol 3-kinase key regulator normal...

Dental enamel is a product of ameloblast cells, which secrete mineralizing organic matrix, composed primarily amelogenin proteins. The amelogenins are thought to be crucial for development normal, highly mineralized enamel. X-chromosomal gene candidate those cases amelogenesis imperfecta, resulting in defective enamel, inheritance X-linked. In this report, kindred described that has C A mutation pro thr change exon 6 the three affected individuals, not found unaffected members kindred....

Hsp47, an endoplasmic reticulum resident protein, has gelatin-binding and procollagen-binding properties been hypothesized to function as a molecular chaperone in regulating procollagen folding and/or assembly. In this report, we further investigate the interaction of Hsp47 with polysome-associated alpha 1(I) chains following antisense treatment 3T6 cells. For these studies, employed phosphorothioate oligodeoxynucleotides directed first five codons that straddle predicted translation...

While the exact mechanisms regulating bone homeostasis are unknown, it is generally accepted that factors with capacity to regulate cell attachment and spreading play a role in osteogenesis. A 44 kDa phosphoprotein (44K BPP), isolated from rat synthesized by osteoblasts, was evaluated for its of fibroblasts. In uncoated plates, enhanced were observed when fibroblasts exposed 44K BPP. The properties different those fibronectin, BPP did not promote type I collagen wells, as seen fibronectin....

The human c-Ha-ras protooncogene product p21C was truncated at the C terminus by 23 amino acids. resulting G-binding domain, p21 (1-166) = p21C', can be crystallized as a complex with slowly hydrolyzing GTP analogues guanosin-5'-[beta,gamma-imido]triphosphate, guanosin-5'-[beta,gamma-methylene]triphosphate, and guanosin-5'-O-(3-thiotriphosphate). We show here that this protein has biochemical properties very similar to those of intact protein. Activating mutations in position 12...

Hsp47 and cyclophilin B (CyPB) are residents of the endoplasmic reticulum (ER). Both these proteins closely associated with polysome-associated α1(I) procollagen chains. possesses chaperone properties early during translation while cis/trans-isomerase CyPB facilitate folding. In this report, we further investigate interaction I export from ER. To inhibit vesicular budding retain within ER, cells were treated heterotrimeric G protein inhibitor mastoparan or calphostin C, a specific...

O ne of the initial events required for regeneration periodontal tissues lost due to disease is establishment connective tissue attachment root surfaces. Thus, considerable research efforts have focused on developing reliable procedures gain new attachment. Our studies focus evaluating agents their ability promote cell and spreading using an in vitro assay. For these human gingival fibroblasts (GF) ligament (PDL) cells, after exposure fibronectin; 44 kilodalton bone phosphoprotein (44K...

Background: Increased collagen and extracellular matrix deposition within the gingiva is main characteristic feature of hereditary gingival fibromatosis (HGF). To date, it not well established if these events are a consequence alterations in other molecules synthesis or disturbances homeostatic equilibrium between degradation molecules. Cytokines important regulators expression profibrogenic genes, including type I its molecular chaperone heat shock protein (Hsp)47 proteolytic enzymes...

Background: Cyclosporin A (CsA) is a widely used immunosuppressant that causes significant side effects including gingival overgrowth. The pathogenesis of this condition not fully understood; however, recent studies show CsA regulates the transcription several cytokines transforming growth factor‐beta 1 (TGF‐β1). In study, we evaluated and TGF‐β1 on human normal (NG) fibroblast proliferation, explored possible autocrine stimulation as cellular regulator proliferation induced by in NG...