A5086086716- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- Circular RNAs in diseases
- SARS-CoV-2 and COVID-19 Research
- Chromosomal and Genetic Variations
- RNA regulation and disease
- Diabetes and associated disorders
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Amyotrophic Lateral Sclerosis Research
- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- Mitochondrial Function and Pathology
- Retinal Development and Disorders
- Cancer-related gene regulation
- Neuroscience and Neural Engineering
- Genetics, Aging, and Longevity in Model Organisms
University of Chinese Academy of Sciences
2018-2025
Institute of Biophysics
2016-2025
Chinese Academy of Sciences
2016-2025
Czech Academy of Sciences, Institute of Biophysics
2021-2023
Affiliated Eye Hospital of Wenzhou Medical College
2022-2023
Wenzhou Medical University
2022-2023
United Lincolnshire Hospitals NHS Trust
2022
University of California, San Diego
2013-2020
Henan Normal University
2020
Nottingham University Hospitals NHS Trust
2020
Spermatogenesis in mammals is characterized by two waves of piRNA expression: one corresponds to classic piRNAs responsible for silencing retrotransponsons and the second wave predominantly derived from nontransposon intergenic regions pachytene spermatocytes, but function these largely unknown. Here, we report involvement instructing massive mRNA elimination mouse elongating spermatids (ES). We demonstrate that a piRNA-induced complex (pi-RISC) containing murine PIWI (MIWI) deadenylase CAF1...
The piRNA machinery is known for its role in mediating epigenetic silencing of transposons. Recent studies suggest that this function also involves piRNA-guided cleavage transposon-derived transcripts. As many piRNAs appear to have the capacity target diverse mRNAs, raises intriguing possibility may act extensively as siRNAs degrade specific mRNAs. To directly test hypothesis, we compared mouse PIWI (MIWI)-associated with experimentally identified cleaved mRNA fragments from testes, and...
Neurodegenerative diseases can occur so early as to affect neurodevelopment. From a cohort of more than 2,000 consanguineous families with childhood neurological disease, we identified founder mutation in four independent pedigrees cleavage and polyadenylation factor I subunit 1 (CLP1). CLP1 is multifunctional kinase implicated tRNA, mRNA, siRNA maturation. Kinase activity the mutant protein was defective, tRNA endonuclease complex (TSEN) destabilized, resulting impaired pre-tRNA cleavage....
Abstract SARS-CoV-2 carries the largest single-stranded RNA genome and is causal pathogen of ongoing COVID-19 pandemic. How folded in virion remains unknown. To fill knowledge gap facilitate structure-based drug development, we develop a situ conformation sequencing technology, named vRIC-seq, for probing viral structure unbiasedly. Using vRIC-seq data, reconstruct tertiary reveal surprisingly “unentangled globule” conformation. We uncover many long-range duplexes higher-order junctions,...
Skeletal muscle stem cells (also known as satellite [SCs]) are essential for regeneration and the regenerative activities of SCs intrinsically governed by gene regulatory mechanisms, but post-transcriptional regulation in remains largely unknown. N(6)-methyladenosine (m6A) modification RNAs is most pervasive highly conserved RNA eukaryotic cells; it exerts powerful impact on almost all aspects mRNA processing that mainly endowed its binding with m6A reader proteins. In this study, we...
Abstract Skeletal muscle has a remarkable ability to regenerate owing its resident stem cells (also called satellite cells, SCs). SCs are normally quiescent; when stimulated by damage, they activate and expand form new fibers. The mechanisms underlying SC proliferative progression remain poorly understood. Here we show that DHX36, helicase unwinds RNA G-quadruplex (rG4) structures, is essential for regeneration regulating expansion. DHX36 (initially named RHAU) barely expressed at quiescence...
Abstract Chemotherapeutics remain the first choice for advanced gastric cancers (GCs). However, drug resistance and unavoidable severe toxicity lead to chemotherapy failure poor prognosis. Long noncoding RNAs (lncRNAs) play critical roles in tumor progression many cancers, including GC. Here, through RNA screening, an apoptotic protease‐activating factor 1 (APAF1)‐binding lncRNA (ABL) that is significantly elevated cancerous GC tissues independent prognostic patients identified. Moreover,...
Heart failure is characterized by the transition from an initial compensatory response to decompensation, which can be partially mimicked transverse aortic constriction (TAC) in rodent models. Numerous signaling molecules have been shown part of program, but relatively little known about decompensation that leads heart failure. Here, we show TAC potently decreases RBFox2 protein mouse heart, and cardiac ablation this critical splicing regulator generates many phenotypes resembling those...