A5001836351- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Muscle Physiology and Disorders
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Prenatal Screening and Diagnostics
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Ruminant Nutrition and Digestive Physiology
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Fetal and Pediatric Neurological Disorders
- Geochemistry and Elemental Analysis
- Cancer-related gene regulation
- Adipose Tissue and Metabolism
- Telomeres, Telomerase, and Senescence
- Reinforcement Learning in Robotics
- Ubiquitin and proteasome pathways
- Pluripotent Stem Cells Research
- Genomic variations and chromosomal abnormalities
- Gene expression and cancer classification
- Mesenchymal stem cell research
Chinese University of Hong Kong, Shenzhen
2014-2025
Peking University
2025
Universidad Autónoma de Madrid
2025
Consejo Superior de Investigaciones Científicas
2025
Chinese University of Hong Kong
2015-2024
Nankai University
2023-2024
Huanggang Normal University
2024
China Rural Technology Development Center
2024
Nanjing Medical University
2022-2024
Hong Kong Science and Technology Parks Corporation
2024
Sequencing plasma DNA from a pregnant woman permits genome-wide scanning for the mutational status of fetus prenatally and noninvasively.
To validate the clinical efficacy and practical feasibility of massively parallel maternal plasma DNA sequencing to screen for fetal trisomy 21 among high risk pregnancies clinically indicated amniocentesis or chorionic villus sampling.Diagnostic accuracy validated against full karyotyping, using prospectively collected archived samples.Prenatal diagnostic units in Hong Kong, United Kingdom, Netherlands.753 pregnant women at who underwent definitive diagnosis by whom 86 had a fetus with 21....
BACKGROUND Tumor-derived DNA can be found in the plasma of cancer patients. In this study, we explored use shotgun massively parallel sequencing (MPS) from patients to scan a genome noninvasively. METHODS Four hepatocellular carcinoma and patient with synchronous breast ovarian cancers were recruited. was extracted tumor tissues, preoperative postoperative samples these analyzed MPS. RESULTS We achieved genomewide profiling copy number aberrations point mutations By detecting quantifying...
Significance Genome-wide hypomethylation is frequently observed in cancers. In this study, we showed that genome-wide analysis plasma using shotgun massively parallel bisulfite sequencing a powerful general approach for the detection of multiple types This particularly attractive because high sensitivity and specificity can be achieved low sequence depth, which practical diagnostically. also used monitoring patients following treatment. The same data further detecting cancer-associated copy...
The gene regulatory information is hardwired in the promoter regions formed by cis-regulatory elements that bind specific transcription factors (TFs). Hence, establishing architecture of plant promoters fundamental to understanding expression. determination circuits controlled each TF and identification sequences for all genes have been identified as two goals Multinational Coordinated Arabidopsis thaliana Functional Genomics Project Steering Committee (June 2002).AGRIS an resource...
Single-nucleotide polymorphisms (SNP) associated with polygenetic disorders, such as breast cancer (BC), can create, destroy, or modify microRNA (miRNA) binding sites; however, the extent to which SNPs interfere miRNA gene regulation and affect susceptibility remains largely unknown. We hypothesize that disruption of target by is a widespread mechanism relevant susceptibility. To test this, we analyzed known be BC risk, in silico vitro, for their ability sites referred these SNPs. identified...
Massively parallel sequencing of DNA molecules in the plasma pregnant women has been shown to allow accurate and noninvasive prenatal detection fetal trisomy 21. However, whether approach is as for diagnosis 13 18 unclear due lack data from a large sample set. We studied 392 pregnancies, among which 25 involved fetus 37 fetus, by massively sequencing. By using our previously reported standard z-score approach, we demonstrated that this could identify 36.0% 73.0% at specificities 92.4% 97.2%,...
Noninvasive prenatal testing using fetal DNA in maternal plasma is an actively researched area. The current generation of tests massively parallel sequencing based on counting sequences originating from different genomic regions. In this study, we explored a approach that the use fragment size as diagnostic parameter. This dependent fact circulating molecules are generally shorter than corresponding molecules. First, performed analysis paired-end and microchip-based capillary...
Cell-free DNA (cfDNA) in human plasma is a class of biomarkers with many current and potential future diagnostic applications. Recent studies have shown that cfDNA molecules are not randomly fragmented possess information related to their tissues origin. Pathologies causing death cells from particular result perturbations the relative distribution affected tissues. Such tissue-of-origin analysis particularly useful development liquid biopsies for cancer. It therefore value accurately...
Predicting the price movement of finance securities like stocks is an important but challenging task, due to uncertainty financial markets. In this paper, we propose a novel approach based on Transformer tackle stock prediction task. Furthermore, present several enhancements for proposed basic Transformer. Firstly, Multi-Scale Gaussian Prior enhance locality Secondly, develop Orthogonal Regularization avoid learning redundant heads in multi-head self-attention mechanism. Thirdly, design...
Skeletal muscle stem cells (also known as satellite [SCs]) are essential for regeneration and the regenerative activities of SCs intrinsically governed by gene regulatory mechanisms, but post-transcriptional regulation in remains largely unknown. N(6)-methyladenosine (m6A) modification RNAs is most pervasive highly conserved RNA eukaryotic cells; it exerts powerful impact on almost all aspects mRNA processing that mainly endowed its binding with m6A reader proteins. In this study, we...
Abstract Gene regulatory pathways converge at the level of transcription, where interactions among genes and between regulators target result in establishment spatiotemporal patterns gene expression. The growing identification direct for key transcription factors (TFs) through traditional high-throughput experimental approaches has facilitated elucidation networks genome level. To integrate this information into a Web-based knowledgebase, we have developed Arabidopsis Regulatory Information...
NF-B signaling is implicated as an important regulator of skeletal muscle homeostasis, but the mechanisms by which this transcription factor contributes to maturation and turnover remain unclear.To gain insight into these mechanisms, gene expression profiling was examined in C2C12 myoblasts devoid activity.Interestingly, even proliferating myoblasts, absence caused pronounced induction several myofibrillar genes, suggesting that functions a negative late-stage differentiation.Although...
Muscle stem cells (MuSCs, satellite cells) are the major contributor to muscle regeneration. Like most adult cells, long-term expansion of MuSCs in vitro is difficult. The vivo regeneration abilities quickly lost after culturing vitro, which prevents potential applications cell-based therapies. Here, we establish a system serially expand for over 20 passages by mimicking endogenous microenvironment. We identified that combination four pro-inflammatory cytokines, IL-1α, IL-13, TNF-α, and...
Long intergenic non-coding RNAs (lincRNAs) are emerging as a novel class of and potent gene regulators. High-throughput RNA-sequencing combined with de novo assembly promises quantity discovery transcripts. However, the identification lincRNAs from thousands assembled transcripts is still challenging due to difficulties separating them protein coding (PCTs).We have implemented iSeeRNA, support vector machine (SVM)-based classifier for lincRNAs. iSeeRNA shows better performance compared other...
Little is known how lincRNAs are involved in skeletal myogenesis. Here we describe the discovery of Linc-YY1 from promoter transcription factor (TF) Yin Yang 1 (YY1) gene. We demonstrate that dynamically regulated during myogenesis vitro and vivo. Gain or loss function C2C12 myoblasts muscle satellite cells alters myogenic differentiation injured muscles has an impact on course regeneration. interacts with YY1 through its middle domain, to evict YY1/Polycomb repressive complex (PRC2) target...
Epigenetic mechanisms play an important role in prenatal development, but fetal tissues are not readily accessible. Fetal DNA molecules present maternal plasma and can be analyzed noninvasively.We applied genomewide bisulfite sequencing via 2 approaches to analyze the methylation profile of at single-nucleotide resolution. The first approach used blood samples polymorphic differences between mother fetus methylome across genome. second cells fractional concentration deduce placental...
The newly developed transcription activator-like effector protein (TALE) and clustered regularly interspaced short palindromic repeats/Cas9 factors (TF) offered a powerful precise approach for modulating gene expression. In this article, we systematically investigated the potential of these new tools in activating stringently silenced pluripotency Oct4 (Pou5f1) mouse human somatic cells. First, with number TALEs sgRNAs targeting various regions promoters, found that most efficient TALE-VP64s...
MicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression in post-transcriptional fashion, and emerging studies support their importance regulating many biological processes, including myogenic differentiation muscle development. miR-29 is a promoting factor during myogenesis but its full spectrum of impact on cells has yet to be explored. Here we describe an analysis affected transcriptome C2C12 using high throughput RNA-sequencing platform. The results reveal not only functions...