A5049648719- Cancer Genomics and Diagnostics
- Prenatal Screening and Diagnostics
- Prostate Cancer Treatment and Research
- Epigenetics and DNA Methylation
- Estrogen and related hormone effects
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- Renal and related cancers
- Single-cell and spatial transcriptomics
- COVID-19 Clinical Research Studies
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Diet and metabolism studies
- Asthma and respiratory diseases
- Pregnancy and preeclampsia studies
- Gut microbiota and health
- Assisted Reproductive Technology and Twin Pregnancy
- MicroRNA in disease regulation
- NF-κB Signaling Pathways
- Tryptophan and brain disorders
- SARS-CoV-2 and COVID-19 Research
- Vascular Malformations and Hemangiomas
- Intracranial Aneurysms: Treatment and Complications
- Cancer Mechanisms and Therapy
- Ectopic Pregnancy Diagnosis and Management
Chinese University of Hong Kong
2017-2025
Nanchang University
2025
First Hospital of Jilin University
2009-2025
Jilin University
2012-2025
Prince of Wales Hospital
2018-2025
Nanjing Maternity and Child Health Care Hospital
2024
Nanjing Medical University
2024
Nanjing University
2024
China Medical University
2024
China Academy of Art
2024
Significance The human placenta is a dynamic and cellular heterogeneous organ, which critical in fetomaternal homeostasis the development of preeclampsia. Previous work has shown that placenta-derived cell-free RNA increases during pregnancy. We applied large-scale microfluidic single-cell transcriptomic technology to comprehensively characterize heterogeneity placentas identified multiple placental cell-type–specific gene signatures. Analysis signature expression maternal plasma enabled...
ABSTRACT The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, creates an urgent need for identifying molecular mechanisms that mediate viral entry, propagation, and tissue pathology. Cell membrane bound angiotensin-converting enzyme 2 (ACE2) associated proteases, transmembrane protease serine (TMPRSS2) Cathepsin L (CTSL), were previously identified as mediators of SARS-CoV2 cellular entry. Here, we assess cell type-specific RNA expression ACE2 , TMPRSS2 CTSL through integrated...
We explored the presence of extrachromosomal circular DNA (eccDNA) in plasma pregnant women. Through sequencing following either restriction enzyme or Tn5 transposase treatment, we identified eccDNA molecules These showed bimodal size distributions peaking at ∼202 and ∼338 bp with distinct 10-bp periodicity observed throughout ranges within both peaks, suggestive their nucleosomal origin. Also, predominance 338-bp peak indicated that had a larger distribution than linear human plasma....
Cell-free DNA (cfDNA) fragmentation patterns contain important molecular information linked to tissues of origin. We explored the possibility using predict cytosine-phosphate-guanine (CpG) methylation cfDNA, obviating use bisulfite treatment and associated risks degradation. This study investigated cfDNA cleavage profile surrounding a CpG (i.e., within an 11-nucleotide [nt] window) analyze methylation. The proportion across positions window appeared nonrandom exhibited correlation with...
Androgen receptor (AR) splice variants (ARVs) are implicated in development of castration-resistant prostate cancer (CRPC). Upregulation ARVs often correlates with persistent AR activity after androgen deprivation therapy (ADT). However, the genomic and epigenomic characteristics ARV-dependent cistrome disease relevance ARV-mediated transcriptome remain elusive. Through integrated chromatin immunoprecipitation coupled sequencing (ChIP-seq) RNA (RNA-seq) analysis, we identified...
Cell-free DNA (cfDNA) fragmentation is nonrandom, at least partially mediated by various nucleases, forming characteristic cfDNA end motifs. However, there a paucity of tools for deciphering the relative contributions cleavage patterns related to underlying factors. In this study, through non-negative matrix factorization algorithm, we used 256 5′ 4-mer motifs identify distinct types patterns, referred as “founder” end-motif profiles (F-profiles). F-profiles were associated with different...
The relationship between placental pathology and the maternal syndrome of preeclampsia is incompletely characterized. Mismatch nutrient supply fetal demands induces stress in syncytiotrophoblast, layer placenta direct contact with blood. Such alters content increases release syncytiotrophoblast extracellular vesicles (STB-EVs) into circulation. We have previously shown 5'-tRNA fragments (5'-tRFs) constitute majority small RNA STB-EVs healthy pregnancy. 5'-tRFs are produced response to...
Measurement of DNA derived from different tissues in the circulating pool can provide important information regarding presence many pathological conditions. However, existing methods involving genome-wide bisulfite sequencing are relatively expensive and may present challenges for large-scale analysis.Through identifying differentially methylated regions liver colon compared with other tissues, we identified 2 markers developed corresponding droplet digital PCR assays. Plasma concentrations...
No AccessJournal of UrologyInvestigative Urology1 Feb 2015The Cistrome and Gene Signature Androgen Receptor Splice Variants in Castration Resistant Prostate Cancer Cells Ji Lu, Peter E. Lonergan, Lucas P. Nacusi, Liguo Wang, Lucy J. Schmidt, Zhifu Sun, Travis Van der Steen, Stephen A. Boorjian, Farhad Kosari, George Vasmatzis, G. Klee, Steven Balk, Haojie Huang, Chunxi Donald Tindall LuJi Lu Department Urology, First Hospital Jilin University, Changchun, People's Republic China Mayo Clinic,...
The role of CD4+ T cells in the ischemic tissues T2D patients remains unclear. Here, we report that patients' vascular density was negatively correlated with number infiltrating after injury. Th1 predominant subset, and Th1-derived IFN-γ TNF-α directly impaired human angiogenesis. We then blocked cell infiltration into both Leprdb/db diet-induced obese mice. Genome-wide RNA sequencing shows an increased proliferative angiogenic capability diabetic ECs tissues. Moreover, wire myography...
Hippo-YAP signaling pathway functions in early lineage differentiation of pluripotent stem cells, but the detailed mechanisms remain elusive. We found that knockout (KO) Mst1 and Mst2, two key components Hippo mouse embryonic cells (ESCs), resulted a disruption into mesendoderm lineage. To further uncover underlying regulatory mechanisms, we performed series ChIP-seq experiments with antibodies against YAP, ESC master transcription factors some characterized histone modification markers as...
Abstract Background Although the characterization of cell-free extrachromosomal circular DNA (eccDNA) has gained much research interest, methylation status these molecules is yet to be elucidated. We set out compare densities plasma eccDNA maternal and fetal origins, between small large molecules. The clearance from circulation was also investigated. Methods developed a sequencing protocol for analysis using tagmentation enzymatic conversion approaches. A restriction enzyme-based approach...
Cell-free extrachromosomal circular DNA (eccDNA) as a distinct topological form from linear has recently gained increasing research interest, with possible clinical applications class of biomarkers. In this study, we aimed to explore the relationship between nucleases and eccDNA characteristics in plasma. By using knockout mouse models deficiencies deoxyribonuclease 1 (DNASE1) or like 3 (DNASE1L3), found that cell-free Dnase1l3-/- mice exhibited larger size distributions than wild-type mice....
Background/Objectives: Diabetes mellitus is often accompanied by mental health complications, including anxiety, depression, and cognitive decline. Recent research suggested that capsaicin, the active component of chili peppers, may influence health. This study aimed to determine effect dietary capsaicin on disorders in a type 1 diabetes (T1D) mouse model, while also exploring potential involvement microbiota-gut-brain axis. Methods: We induced T1D mice using streptozotocin (STZ)...
The methoxycarbonylation of aniline (AN) with dimethyl carbonate (DMC) to produce methyl N-phenylcarbamate (MPC), a key intermediate in non-phosgene diphenylmethane diisocyanate (MDI) synthesis, is facilitated using newly developed CuO-CeO 2 ...
Introduction The aim of the study was to explore potential factors affecting emergence sleep disorders in patients with renal failure. Material and methods A cross-sectional approach employed order evaluate relationship between failure disorders, validate findings through Mendelian randomization (MR) analysis. Furthermore, we utilized a two-stage MR methodology quantify specific contribution creatinine, mediated by apnea syndrome, development Results In multivariate adjusted logistic...
Urinary cell-free DNA (ucfDNA) is a valuable resource for truly non-invasive liquid biopsy. UcfDNA comprises transrenal ucfDNA passing from the bloodstream through glomeruli and locally shed urinary-tract ucfDNA. Understanding their differences in characteristics may enable new diagnostic applications. We analyzed 136 samples healthy controls, pregnant women, patients with chronic kidney diseases (CKDs), bladder cancer using massively parallel sequencing. Fragmentomic including fragment...
IL-1 family regulatory cytokine IL-37b can suppress innate immunity and inflammatory activity in diseases. In this study, showed remarkable vitro suppression of tumor necrosis factor-α, IL-1β, IL-6, CCL2, CXCL8 production the coculture human primary eosinophils bronchial epithelial BEAS-2B cells with stimulation bacterial toll-like receptor-2 ligand peptidoglycan, while antagonizing activation intracellular nuclear factor-κB, PI3K-Akt, extracellular signal-regulated kinase 1/2, suppressing...
COVID-19 first broke out in China and spread rapidly over the world.
To elucidate the effects of age on expression levels receptor activator nuclear factor-κB ligand (RANKL) and osteoclasts in periodontal ligament during orthodontic mechanical loading post-orthodontic retention.The study included 20 male Sprague-Dawley rats, ten young group (aged four to five weeks) adult 18 weeks). In each rat, upper-left first molar was subjected a seven-day force followed by retention period. The upper-right served as control. amount tooth movement measured after...
Prostate carcinoma is a global health problem and estimated to be diagnosed in 1.1 million men/year, making this malignancy the second most frequently cancer males worldwide. micro RNAs (miRNAs) are small non-coding that negatively regulate gene expression at post-transcriptional level. miRNAs contribute development progression, expressed differently normal tissues cancers. In present study, biological function of miR-106a human prostate associated regulatory mechanisms were investigated....